Acidic Aqueous Extraction of Hypothalamic Luteinizing Hormone Releasing Hormone to Study Biological Effects
1974 ◽
Vol 52
(3)
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pp. 754-758
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Keyword(s):
Several aqueous solvent systems were tested for their efficiency in extracting luteinizing hormone releasing hormone (LH-RH) from rat hypothalamus. Although LH-RH is a water-soluble decapeptide, neutral distilled water extracted only 10% of the LH-RH obtained using acid extraction methods. The efficiency of the acid extraction procedure suggests that in the hypothalamus the releasing hormone is bound to a relatively large molecular weight compound. Using the acidic extraction procedure, we found that hypothalamic LH-RH content is significantly lower in the castrated animal than in the normal rat.
2001 ◽
Vol 98
(4)
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pp. 1829-1834
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1972 ◽
Vol 49
(6)
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pp. 1467-1473
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1971 ◽
Vol 32
(1)
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pp. 123-126
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Keyword(s):
1978 ◽
Vol 26
(7)
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pp. 542-544
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