In situ molecular weight determination of rat brain [3H]phenytoin binding sites

1985 ◽  
Vol 63 (5) ◽  
pp. 515-516 ◽  
Author(s):  
Lawrence Spero

Using irradiation inactivation analysis of specific [3H]phenytoin binding to rat brain we have demonstrated that there are two different binding sites involved, with molecular weights of 73 238 ± 1535 (higher affinity site) and 108 121 ± 6935 (lower affinity site).




Author(s):  
Yan-Yan Tu ◽  
Xue-Ting Wan ◽  
Jie Huan ◽  
Xiang Zhu ◽  
Xiao-Hong Li ◽  
...  


1983 ◽  
Vol 38 (9-10) ◽  
pp. 887-890 ◽  
Author(s):  
M. J. Carter ◽  
K. Baczko

A recent examination of measles virus mRNA molecules has shown that the nucleocapsid and haemagglutin messengers are of the size expected from a consideration of their protein products. How ever, the mRNA for membrane protein is approximately 50% larger than the size required. The molecular weight of matrix protein has been determined by SDS-polyacrylamide gel electrophoresis, and this procedure can lead to an underestimation of the true size of hydrophobic molecules which show increased SDS binding. It is therefore appropriate to examine the molecular weight determination of this protein to exclude such an artefactual discrepancy in mRNA and protein sizes. We report here that measles virus membrane protein does not shown such anomalous behaviour and confirm that the size discrepancy is a true phenomenon.





RSC Advances ◽  
2016 ◽  
Vol 6 (110) ◽  
pp. 108635-108644 ◽  
Author(s):  
Xian-En Zhao ◽  
Yongrui He ◽  
Ping Yan ◽  
Na Wei ◽  
Renjun Wang ◽  
...  

In situ UA-DDLLME coupled with UHPLC-MS/MS has been developed for simultaneous determination of neurotransmitters and baicalein from Parkinson's disease rats.



1975 ◽  
Vol 39 (8) ◽  
pp. 1527-1531
Author(s):  
Zen-ichiro Hamauzu ◽  
Yukio Kamazuka ◽  
Hirokazu Kanazawa ◽  
Daizo Yonezawa




Sign in / Sign up

Export Citation Format

Share Document