Effects of salmon gonadotrophin (SG-G100) on plasma lipids in the goldfish, Carassius auratus

Female goldfish were held under conditions of 12 °C and a 12 h light: 12 h dark photoperiod during three phases of the sexual cycle. A pretreatment blood sample was taken after 2 weeks of acclimation. Comparison of pretreatment levels of plasma lipids from the three experiments revealed that the concentration of triglycerides (TG) increased and total cholesterol (TC) decreased with increasing ovarian size. Plasma lipid phosphorus (LP) levels were slightly higher in sexually mature fish than in sexually regressed fish. Two weeks after the pretreatment blood sample, the fish were injected intraperitoneally with salmon gonadotrophin (SG-G100) or control solution for 3 days, after which a posttreatment blood sample was taken. In sexually maturing fish injection of SG-G100 caused increased plasma TG levels (compared with pretreatment) in fish with small ovaries, changing to decreased levels in fish with larger ovaries. A similar effect was also seen in maturing fish with plasma TC; these effects were abolished by castration or by keeping fish at 21 °C. SG-G100 had little effect on plasma LP. The results suggest that gonadotrophin causes a net mobilization of lipid in fish with small ovaries (presumably via sex steroids) and accelerated ovarian uptake of lipid in fish with larger ovaries.

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Effects of sex steroids on plasma lipids in the goldfish, Carassius auratus

Canadian Journal of Zoology ◽

10.1139/z80-135 ◽

1980 ◽

Vol 58 (6) ◽

pp. 967-972 ◽

Cited By ~ 32

Author(s):

Murray D. Wiegand ◽

R. E. Peter

Keyword(s):

Sex Steroids ◽

Carassius Auratus ◽

Plasma Lipids ◽

Plasma Lipid ◽

Plasma Triglyceride ◽

Control Fish ◽

Warm Temperature ◽

Plasma Total Cholesterol ◽

Goldfish Carassius Auratus ◽

Phosphorus Levels

The effects of sex steroids on plasma lipids in female goldfish were studied in sexually regressed and maturing fish at 12 °C, and in recent postovulatory-regressed fish at 21 °C. Intraperitoneal injection of oestrone (E1), but not oestradiol (E2), raised plasma triglyceride (TG) concentrations in regressed fish, but oestrogen had no effect on plasma TG in either maturing fish at 12 °C or in recent postovulatory–regressed fish at 21 °C. Progesterone injection caused high levels of plasma TG in maturing fish at 12 °C. Fish injected with E1or E2 had higher plasma total cholesterol (TC) levels posttreatment compared with control fish in both experiments at 12 °C, but E1 and E2 were without effect on plasma TC at 21 °C. Both E1 and E2 raised plasma lipid phosphorus levels in all three experiments. Testosterone generally had little effect on plasma lipids. These results support the hypothesis that oestrogen is involved in lipid mobilization in teleosts, and it appears that this effect is sensitive to warm temperature. There was no support for a mammalian-like, progesterone-stimulated system for clearance of plasma TG in the female goldfish.

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Waterborne fluoxetine disrupts the reproductive axis in sexually mature male goldfish, Carassius auratus

Aquatic Toxicology ◽

10.1016/j.aquatox.2010.08.016 ◽

2010 ◽

Vol 100 (4) ◽

pp. 354-364 ◽

Cited By ~ 83

Author(s):

Jan A. Mennigen ◽

Wudu E. Lado ◽

Jake M. Zamora ◽

Paula Duarte-Guterman ◽

Valérie S. Langlois ◽

...

Keyword(s):

Carassius Auratus ◽

Mature Male ◽

Goldfish Carassius Auratus ◽

Reproductive Axis ◽

Sexually Mature

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Diel cyclic hypoxia alters plasma lipid dynamics and impairs reproduction in goldfish (Carassius auratus)

Fish Physiology and Biochemistry ◽

10.1007/s10695-017-0401-0 ◽

2017 ◽

Vol 43 (6) ◽

pp. 1677-1688 ◽

Cited By ~ 6

Author(s):

Aritra Bera ◽

Paramita Banerjee Sawant ◽

Subrata Dasgupta ◽

N. K. Chadha ◽

Bhawesh T. Sawant ◽

...

Keyword(s):

Carassius Auratus ◽

Plasma Lipid ◽

Goldfish Carassius Auratus ◽

Lipid Dynamics

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The effect of 17β-estradiol on spontaneous ovulation in the goldfish, Carassius auratus

Canadian Journal of Zoology ◽

10.1139/z85-446 ◽

1985 ◽

Vol 63 (12) ◽

pp. 2979-2981 ◽

Cited By ~ 8

Author(s):

N. W. Pankhurst ◽

N. E. Stacey

Keyword(s):

Body Weight ◽

Carassius Auratus ◽

Mature Female ◽

Goldfish Carassius Auratus ◽

17Β Estradiol ◽

Gonadotropin Surge ◽

Sexually Mature

Sexually mature female goldfish were treated daily with 17β-estradiol in oil (2 μg∙g body weight−1) or given a single silastic pellet containing 17β-estradiol (50 μg∙g−1) and held under conditions which promote spontaneous ovulation. Levels of 17β-estradiol were found to be significantly elevated by the treatments during the period prior to ovulation. However, neither of the 17β-estradiol treatments had any effect on gonadotropin levels or the number of fish ovulating. A decline in the level of serum 17β-estradiol does not appear to be a critical requisite for the occurrence of the preovulatory gonadotropin surge in goldfish.

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SEM of Hepatocytes and Biliary Passages in Goldfish Liver

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080213 ◽

1977 ◽

Vol 35 ◽

pp. 556-557

Author(s):

Waykin Nopanitaya ◽

Joe W. Grisham ◽

Johnny L. Carson

Keyword(s):

Carassius Auratus ◽

Cell Layer ◽

Three Dimensional ◽

Cell Types ◽

Biliary System ◽

Fish Food ◽

Commercial Fish ◽

Goldfish Carassius Auratus ◽

Commercial Fish Food

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.

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Induction of Gynogenesis and Androgenesis in Goldfish Carassius auratus (var. oranda)

Reproduction in Domestic Animals ◽

10.1046/j.1439-0531.2001.d01-36.x ◽

2001 ◽

Vol 36 (3-4) ◽

pp. 195-198 ◽

Cited By ~ 14

Author(s):

I Paschos ◽

L Natsis ◽

C Nathanailides ◽

I Kagalou ◽

E Kolettas

Keyword(s):

Carassius Auratus ◽

Goldfish Carassius Auratus

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Cardioprotective Effects of Diet with Different Grains on Lipid Profiles and Antioxidative System in Obesity-Induced Rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000097 ◽

2012 ◽

Vol 82 (2) ◽

pp. 85-93 ◽

Cited By ~ 9

Author(s):

Y. Kim ◽

H. Shin ◽

S. Lee

Keyword(s):

Aortic Wall ◽

Plasma Lipids ◽

Antioxidant Activities ◽

Plasma Lipid ◽

Density Lipoprotein ◽

Thiobarbituric Acid ◽

Lipid Profiles ◽

Dietary Lipids ◽

Male Rats

In the present study, the nutritional quality of four grains including adlay (AD), buckwheat (BW), glutinous barley (GB), and white rice (WR) were evaluated in terms of plasma lipid parameters, gut transit time, and thickness of the aortic wall in rats. The rats were then raised for 4 weeks on the high-fat diet based on the American Institute of Nutrition-93 (AIN-93 G) diets containing 1 % cholesterol and 20 % dietary lipids. Forty male rats were divided into 4 groups and raised for 4 weeks with a diet containing one of the following grains: WR, AD, BW, or WB. The level of thiobarbituric acid-reactive substances (TBARS) in liver was shown to be higher in rats by the order of those fed WR, AD, GB, and BW. This indicates that other grains decreased oxidative stress in vivo more than WR. The superoxide dismutase, glutathione, glutathione peroxidase, and glutathione reductase levels in the AD, BW, and GB groups were significantly higher than those in the WR group (p < 0.05). Plasma lipid profiles differed significantly according to grain combination, and decreased aortic wall thickness was consistent with the finding of decreased plasma low-density lipoprotein cholesterol (LDL-C) (p < 0.05) and increased high-density lipoprotein (HDL-C) in rats fed AD, BW, and GB (p < 0.001). The antioxidant and hypolipidemic capacities of grains are quite high, especially those of adlay, buckwheat, and glutinous barley. In conclusion, this study has demonstrated that the whole grains had a cardioprotective effect. This effect was related to several mechanisms that corresponded to lowering plasma lipids, decreasing TBARS, and increasing antioxidant activities.

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