A taxonomic reevaluation of North American Daphnia (Crustacea: Cladocera). III. The D. catawba complex
Despite the importance of Daphnia in freshwater zooplankton assemblages, species boundaries in the genus are unclear. This study verifies the taxonomic validity of D. catawba by establishing its genetic divergence from other species of Daphnia that occur in eastern North America. In addition, it reveals the presence of a second, closely allied species, D. minnehaha, which had previously been placed in synonomy with D. pulex. Daphnia catawba and D. minnehaha share a preference for acidic habitats and are restricted to the deciduous and boreal forest regions of the eastern portion of the continent, where D. catawba is restricted to lakes, while D. minnehaha occurs in ponds. Both species reproduce by cyclic parthenogenesis and, based on the extent of their allozyme differentiation, last had a common ancestor more than 7 million years ago. Populations of D. minnehaha fall into two genetic clades; those from the Great Lakes watershed are morphologically divergent and have much lower levels of genotypic diversity than those from eastern Canada and the New England states.