Long Term Safety and Immunological Effects of a Nanobiotherapeutic, Bovine Poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], After Four Weekly 5% Blood Volume Toploading Followed by a Challenge of 30% Exchange Transfusion

Since the healing capacity of articular cartilage is limited, it is important to develop cell-based therapies for the repair of cartilage. Although synthetic or animal-derived scaffolds are frequently used for effective cell delivery long-term safety and efficiency of such scaffolds still remain unclear. We have been studying on a scaffold-free tissue engineered construct (TEC) bio-synthesized from synovium-derived mesenchymal stem cells (MSCs) [1]. As the TEC specimen is composed of cells with their native extracellular matrix, we believe that it is free from concern regarding long term immunological effects. our previous studies indicated that a porcine partial thickness chondral defect was successfully repaired with TEC but that the compressive property of the TEC-treated cartilage-like repaired tissue was different from normal cartilage in both immature and mature animals. Imura et al. found that the permeability of the immature porcine cartilage-like tissues repaired with TEC recovered to normal level for 6 months except the superficial layer [2]. Therefore, the present study was performed to determine the depth-dependent permeability of mature porcine cartilage-like tissue repaired with TEC. Moreover, we investigated the effect of difference of permeability on the compressive property of articular cartilage using a finite element analysis (FEM).

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Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress

Zygote ◽

10.1017/s0967199419000467 ◽

2019 ◽

Vol 27 (6) ◽

pp. 432-435

Author(s):

Thais Rose dos Santos Hamilton ◽

Gabriela Esteves Duarte ◽

José Antonio Visintin ◽

Mayra Elena Ortiz D’Ávila Assumpção

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Antioxidant Enzymes ◽

Heat Stress ◽

Glutathione Peroxidase ◽

Cell Types ◽

Treated Group ◽

Oxidative Balance ◽

Testicular Cells

SummaryLong-term heat stress (HS) induced by testicular insulation generates oxidative stress (OS) on the testicular environment; consequently activating antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The aim of this work was to immunolocalize antioxidant enzymes present in different cells within the seminiferous tubule when rams were submitted to HS. Rams were divided into control (n = 6) and treated group (n = 6), comprising rams subjected to testicular insulation for 240 h. After the testicular insulation period, rams were subjected to orchiectomy. Testicular fragments were submitted to immunohistochemistry for staining against SOD, GR and GPx enzymes. We observed immunolocalization of GPx in more cell types of the testis after HS and when compared with other enzymes. In conclusion, GPx is the main antioxidant enzyme identified in testicular cells in an attempt to maintain oxidative balance when HS occurs.

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Effects of long-term blockade of angiotensin converting enzyme with captopril (SQ14,225) on hemodynamics and circulating blood volume in SHR.

Hypertension ◽

10.1161/01.hyp.2.3.299 ◽

1980 ◽

Vol 2 (3) ◽

pp. 299-303 ◽

Cited By ~ 70

Author(s):

H Koike ◽

K Ito ◽

M Miyamoto ◽

H Nishino

Keyword(s):

Angiotensin Converting Enzyme ◽

Blood Volume ◽

Converting Enzyme ◽

Circulating Blood Volume

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Long-term hemodynamic actions of atrial natriuretic factor (99-126) in conscious sheep

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1988.254.4.h811 ◽

1988 ◽

Vol 254 (4) ◽

pp. H811-H815 ◽

Cited By ~ 4

Author(s):

D. G. Parkes ◽

J. P. Coghlan ◽

J. G. McDougall ◽

B. A. Scoggins

Keyword(s):

Blood Pressure ◽

Cardiac Output ◽

Stroke Volume ◽

Blood Volume ◽

Total Peripheral Resistance ◽

Atrial Natriuretic Factor ◽

Peripheral Resistance ◽

Natriuretic Factor ◽

Atrial Natriuretic

The hemodynamic and metabolic effects of long-term (5 day) infusion of human atrial natriuretic factor (ANF) were examined in conscious chronically instrumented sheep. Infusion of ANF at 20 micrograms/h, a rate below the threshold for an acute natriuretic effect, decreased blood pressure by 9 +/- 1 mmHg on day 5, associated with a fall in calculated total peripheral resistance. On day 1, ANF reduced cardiac output, stroke volume, and blood volume, effects that were associated with an increase in heart rate and calculated total peripheral resistance and a small decrease in blood pressure. On days 4 and 5 there was a small increase in urine volume and sodium excretion. On day 5 an increase in water intake and body weight was observed. No change was seen in plasma concentrations of renin, arginine vasopressin, glucose, adrenocorticotropic hormone, or protein. This study suggests that the short-term hypotensive effect of ANF results from a reduction in cardiac output associated with a fall in both stroke volume and effective blood volume. However, after 5 days of infusion, ANF lowers blood pressure via a reduction in total peripheral resistance.

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