THE INTERACTION BETWEEN ELECTRORHOLOGICAL PARTICLES IN AC ELECTRIC FIELD STUDIED USING OPTICAL TWEEZERS

2002 ◽  
Author(s):  
J. XU ◽  
X. DONG ◽  
Z.Y. CHEN ◽  
Y.G. JIANG ◽  
L.W. ZHOU
Keyword(s):  
Electric Field ◽  
Optical Tweezers ◽  
Ac Electric Field

Combination of Au Dielectrophoresis Chip and Optical Tweezers for Cell Culture

2015 ◽  
Vol 656-657 ◽  
pp. 549-553
Author(s):  
Kyohei Nishimoto ◽  
Kozo Taguchi
Keyword(s):  
Electric Field ◽  
Optical Tweezers ◽  
Cell Separation ◽  
Low Cost ◽  
Three Dimensional ◽  
Uniform Electric Field ◽  
Objective Lens ◽  
Separation System ◽  
Ac Electric Field ◽  
Viable Cells

Dielectrophoresis (DEP) force will arise when an inhomogeneous AC electric field with sinusoidal wave is applied to microelectrodes. By using DEP, we could distinguish between viable and non-viable cells by their movement through a non-uniform electric field. In this paper, we propose a yeast cell separation system, which utilizes an Au DEP chip and an optical tweezers. The Au DEP chip is planar quadrupole microelectrodes, which were fabricated by Au thin-film and a box cutter. This fabrication method is low cost and simpler than previous existing methods. The tip of the optical tweezers was fabricated by dynamic chemical etching in a mixture of hydrogen fluoride and toluene. The optical tweezers has the feature of high manipulation performance. That does not require objective lens for focusing light because the tip of optical tweezers has conical shape. By using both the Au DEP chip and optical tweezers, we could obtain three-dimensional manipulation of specific cells after viability separation.

THE INTERACTION BETWEEN ELECTRORHEOLOGICAL PARTICLES IN AC ELECTRIC FIELD STUDIED USING OPTICAL TWEEZERS

2002 ◽  
Vol 16 (17n18) ◽  
pp. 2300-2306
Author(s):  
J. XU ◽  
X. DONG ◽  
Z. Y. CHEN ◽  
Y. G. JIANG ◽  
L. W. ZHOU
Keyword(s):  
Image Processing ◽  
Electric Field ◽  
Optical Tweezers ◽  
High Speed ◽  
Sampling Rate ◽  
Interaction Strength ◽  
Processing System ◽  
Dipole Interaction ◽  
High Speed Imaging ◽  
Ac Electric Field

The dipole interaction between a pair of electrorheological (ER) particles under an external AC electric field was confirmed in a dynamic case using optical tweezers positioning and a system of high speed imaging and image processing. By measuring the interactions between two micron-sized ER particles with both central distance and structure forming time, the structure response time and the interaction strength were obtained for the particles under an AC electric field. The spacial resolution of the optical tweezers-high speed imaging-image processing system is 0.26μm. The sampling rate of the high-speed video recorder is up to 8000 frame/s with the corresponding time resolution being 0.125 ms.

Simulation of TiO 2 particle trajectory in AC electric field

2015 ◽  
Vol 108 ◽  
pp. 183-191 ◽  
Author(s):  
Reza Riahifar ◽  
Babak Raissi ◽  
Cyrus Zamani ◽  
Ehsan Marzbanrad
Keyword(s):  
Electric Field ◽  
Particle Trajectory ◽  
Ac Electric Field

Electrically induced reversible viscosity change in hectorite aqueous dispersion under an AC electric field

2014 ◽  
Vol 99 ◽  
pp. 160-163 ◽  
Author(s):  
Hiroshi Kimura ◽  
Mao Ueno ◽  
Shinya Takahashi ◽  
Akira Tsuchida ◽  
Keiichi Kurosaka
Keyword(s):  
Electric Field ◽  
Aqueous Dispersion ◽  
Viscosity Change ◽  
Ac Electric Field

Mechanism of Cell Lysis in Microfluidic Channel With Integrated Nanocomposite Electrodes

2013 ◽  
Author(s):  
Madhusmita Mishra ◽  
Anil Krishna Koduri ◽  
Aman Chandra ◽  
D. Roy Mahapatra ◽  
G. M. Hegde
Keyword(s):  
Electric Field ◽  
Microfluidic Channel ◽  
Cell Lysis ◽  
Bacterial Cells ◽  
Nano Composite ◽  
Time Resolved ◽  
Ac Electric Field ◽  
Cell Dispersion ◽  
Electrical Lysis ◽  
Nanocomposite Electrodes

This paper reports on the characterization of an integrated micro-fluidic platform for controlled electrical lysis of biological cells and subsequent extraction of intracellular biomolecules. The proposed methodology is capable of high throughput electrical cell lysis facilitated by nano-composite coated electrodes. The nano-composites are synthesized using Carbon Nanotube and ZnO nanorod dispersion in polymer. Bacterial cells are used to demonstrate the lysis performance of these nanocomposite electrodes. Investigation of electrical lysis in the microchannel is carried out under different parameters, one with continuous DC application and the other under DC biased AC electric field. Lysis in DC field is dependent on optimal field strength and governed by the cell type. By introducing the AC electrical field, the electrokinetics is controlled to prevent cell clogging in the micro-channel and ensure uniform cell dispersion and lysis. Lysis mechanism is analyzed with time-resolved fluorescence imaging which reveal the time scale of electrical lysis and explain the dynamic behavior of GFP-expressing E. coli cells under the electric field induced by nanocomposite electrodes. The DNA and protein samples extracted after lysis are compared with those obtained from a conventional chemical lysis method by using a UV–Visible spectroscopy and fluorimetry. The paper also focuses on the mechanistic understanding of the nano-composite coating material and the film thickness on the leakage charge densities which lead to differential lysis efficiency.

Deformation of liquid crystal droplets under the action of an external ac electric field

2001 ◽  
Vol 64 (2) ◽  
Author(s):  
B. I. Lev ◽  
V. G. Nazarenko ◽  
A. B. Nych ◽  
D. Schur ◽  
P. M. Tomchuk ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Electric Field ◽  
Ac Electric Field
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