The Hepatoprotective Effects of Solanum alatum Moench. on Acetaminophen-induced Hepatotoxicity in Mice

Solanum alatum Moench. has been shown to have a protective effect against carbon tetrachloride (CCI4)-induced liver injury. Solanum alatum treatment (100 mg/kg, p.o) decreased the elevation of serum alanine aminotransferase (ALT; GPT) and aspartate aminotransferase (AST; GOT) induced by acetaminophen (paracetamol) (600 mg/kg, i.p.) administration. It also decreased the extent of visible necrosis in liver tissue. In addition, Solanum alatum treatment restored hepatic glutathione (GSH) depletion induced by acetaminophen (600 mg/kg, i.p.) administration. Microsomal enzyme levels such as P-450, reductas and aniline hydroxylation enzyme were also restored to normal levels after Solanum alatum administration. The hepatoprotective mechanism may function through direct binding with acetaminophen toxic metabolites, decreasing the attraction of acetaminophen metabolites for other cellular GSH or thiol protein. Additionally, Solanum alatum treatment increased the concentration of hepatic GSH and maintained a high level activity of GSTase, which led to acceleration of the excretion of toxic acetaminophen metabolites.

Download Full-text

Explore the mechanism of Swertia mussotii Franch. for hepatoprotective effects with iTRAQ-LC-MS/MS

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207323666201020111301 ◽

2020 ◽

Vol 23 ◽

Author(s):

Haixia Yun ◽

Xinyu Wu ◽

Yiwei Ding ◽

Wendou Xiong ◽

Xianglan Duan ◽

...

Keyword(s):

Lipid Metabolism ◽

Carbon Tetrachloride ◽

Liver Injury ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Total Bilirubin ◽

Acute Liver Injury ◽

Proteome Analysis ◽

Ppi Networks ◽

Hepatoprotective Effects

Background and Objective : A Tibetan traditional herb named Swertia mussotii Franch., also called “Zangyinchen” by the local people of Qinghai-Tibet area, has been used to protect the liver from injury for many years. However, the curative effect and molecular mechanism of the herb have not been demonstrated clearly. Materials and Methods: In our study, serum alanine aminotransferase, aspartate aminotransferase, total bilirubin levels were examined after S. mussotii Franch. treatment in the acute liver injury of the carbon tetrachloride-induced rat model. Then, Proteome Analysis was applied to explore the potential mechanism of SMT for hepatoprotective effects after iTRAQLC-MS/MS analysis (isobaric tag for relative and absolute quantification-liquid chromatograph-mass spectrometer with tandem mass spectrometry). Results: Serum results showed, alanine aminotransferase, aspartate aminotransferase, total bilirubin levels of rats with acute liver injury were all improved with SMT treatment. Moreover, Proteome Analysis suggested that, with S. Mussotii Franch. treatment, the levels of lipid catabolic process and lipid homeostasis were all enhanced. And the results of protein-protein interaction (PPI) analysis illustrated that these proteins assembled in PPI networks were found almost significantly enriched in response to lipid, negative regulation of lipase activity, response to lipopolysaccharide etc. Furthermore, the downregulated MRP14 and MRP8 proteins were found involved in the lipid metabolism, which may indicate the mechanism of SMT protection liver from ALI induced by carbon tetrachloride. Conclusion: SMT herb could play a role in hepatoprotection and alleviate the effect of acute liver injury by impacting the lipid metabolism associated biological process.

Download Full-text

Hepatoprotective Effects of Polysaccharides Isolated from Phellinus gilvus Against Carbon Tetrachloride-induced Liver Injury in Rats

Toxicological Research ◽

10.5487/tr.2009.25.1.029 ◽

2009 ◽

Vol 25 (1) ◽

pp. 29-33 ◽

Cited By ~ 2

Author(s):

Seung-Chun Park ◽

Yong-Pil Cheon ◽

Wha-Young Son ◽

Man-Hee Rhee ◽

Tae-Wan Kim ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Hepatoprotective Effects ◽

Phellinus Gilvus

Download Full-text

Hepatoprotective effects of hydroethanolic extracts of Crocus sativus tepals, stigmas and leaves on carbon tetrachloride induced acute liver injury in rats

Physiology and Pharmacology ◽

10.32598/ppj.25.2.30 ◽

2021 ◽

Vol 25 (2) ◽

pp. 178-188

Author(s):

Sabir Ouahhoud ◽

◽

Ilham Touiss ◽

Amine Khoulati ◽

Iliass Lahmass ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Total Bilirubin ◽

Liver Weight ◽

Crocus Sativus ◽

Male Rats ◽

Control Group ◽

Distilled Water ◽

Relative Liver Weight ◽

Hepatoprotective Effects

Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and leaves of Crocus sativus on carbon tetrachloride (CCL4) induced liver injury in rats. Methods: Hydroethanolic extracts of Crocus sativus (stigmas, tepals and leaves) were administrated daily for 14 days by oral gavage. In the present study, 30 male rats divided into five groups were treated as 1: normal rats gavaged with distilled water; 2: intoxicated rats gavaged with distilled water and injected with CCL4; 3: rats treated with stigmas extract and injected with CCL4; 4: rats treated with tepal extract and injected with CCL4; 5: rats treated with leaf extract and injected with CCL4. Bodyweight and the relative liver weight were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol, triglycerides, bilirubin direct and total, total protein, albumin, urea and creatinine measured in plasma. Malondialdehyde (MDA) was quantified in liver homogenate. Results: The experimental data showed that the stigmas and tepals extracts significantly prevented weight body loss and improved the relative liver weight. They significantly protected against elevation of ALT, AST, direct bilirubin, total bilirubin, LDH, ALP, creatinine and MDA. Also, they enhanced significantly total proteins and albumin compared to the CCL4 control group. Moreover, leaves reduced ALT, AST, total bilirubin, LDH and MDA significantly. Conclusion: In conclusion, these results suggest that tepals, stigmas, and leaves extracts of Crocus sativus have hepatoprotective effects on CCL4 induced liver injury in rats.

Download Full-text

Hepatoprotective effects of propolis extract on carbon tetrachloride-induced liver injury in rats

Phytotherapy Research ◽

10.1002/ptr.2650090207 ◽

1995 ◽

Vol 9 (2) ◽

pp. 114-117 ◽

Cited By ~ 34

Author(s):

R. González ◽

I. Corcho ◽

D. Remirez ◽

S. Rodriguez ◽

O. Ancheta ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Propolis Extract ◽

Hepatoprotective Effects

Download Full-text

Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats

Food and Chemical Toxicology ◽

10.1016/j.fct.2012.10.034 ◽

2013 ◽

Vol 52 ◽

pp. 83-90 ◽

Cited By ~ 42

Author(s):

Vladimir Mihailović ◽

Mirjana Mihailović ◽

Aleksandra Uskoković ◽

Jelena Arambašić ◽

Danijela Mišić ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Hepatoprotective Effects

Download Full-text

Hepatoprotective effects of total saponins isolated from Taraphochlamys affinis against carbon tetrachloride induced liver injury in rats

Food and Chemical Toxicology ◽

10.1016/j.fct.2011.12.009 ◽

2012 ◽

Vol 50 (3-4) ◽

pp. 713-718 ◽

Cited By ~ 37

Author(s):

QuanFang Huang ◽

ShiJun Zhang ◽

Li Zheng ◽

Min He ◽

RenBin Huang ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Hepatoprotective Effects

Download Full-text

Hepatoprotective effects of almond oil against carbon tetrachloride induced liver injury in rats

Food Chemistry ◽

10.1016/j.foodchem.2010.09.062 ◽

2011 ◽

Vol 125 (2) ◽

pp. 673-678 ◽

Cited By ~ 43

Author(s):

Xiao-Yan Jia ◽

Qing-An Zhang ◽

Zhi-Qi Zhang ◽

Yan Wang ◽

Jiang-Feng Yuan ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Almond Oil ◽

Hepatoprotective Effects

Download Full-text

Effect of Pretreatment of Rats with an Urinary Preparation on Liver Injuries Induced by Carbon Tetrachloride and α-Naphthylisothiocyanate

The American Journal of Chinese Medicine ◽

10.1142/s0192415x98000385 ◽

1998 ◽

Vol 26 (03n04) ◽

pp. 343-351 ◽

Cited By ~ 7

Author(s):

Tung-Yuan Lai ◽

Yueh-Wern Wu ◽

Jaung-Geng Lin ◽

Wen-Chuan Lin

Keyword(s):

Lipid Peroxidation ◽

Carbon Tetrachloride ◽

Liver Injury ◽

Oral Administration ◽

Human Urine ◽

Acute Liver Injury ◽

Indirect Pathway ◽

Liver Injuries ◽

Hepatic Glutathione ◽

Biochemical Observation

The effect of oral administration of a preparation of human urine (PHU) on the progression of acute liver injury was examined in rats intoxicated with carbon tetrachloride (CCl4) and α-naphthylisothiocyanate (ANIT). PHU protected the liver from CCl4-induced injury as judged by morphological and biochemical observations. In contrast, PHU aggravated ANIT-induced injury as judged also by morphological and biochemical observation. PHU prevented the increase in hepatic glutathione (GSH) and lipid peroxidation induced by CCl4. But PHU enhanced the increase in hepatic GSH caused by ANIT. These results indicate that the effect of PHU on hepatic GSH concentrations is through an indirect pathway. Clinical application of PHU on hepatitis should be explored further.

Download Full-text

Anti-Inflammatory and Hepatoprotective Effects of Ganoderma lucidum Polysaccharides against Carbon Tetrachloride-Induced Liver Injury in Kunming Mice

Pharmacology ◽

10.1159/000493896 ◽

2019 ◽

Vol 103 (3-4) ◽

pp. 143-150 ◽

Cited By ~ 8

Author(s):

Yu-Sheng Chen ◽

Quan-Zhan Chen ◽

Zhen-Jiong Wang ◽

Chun Hua

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Liver Tissue ◽

Ganoderma Lucidum ◽

Acute Liver Injury ◽

Liver Weight ◽

Inflammatory Factors ◽

Ganoderma Lucidum Polysaccharides ◽

Hepatoprotective Effects ◽

Anti Inflammatory

Background: Ganoderma lucidum Polysaccharides (GLPS) were found to possess various pharmacological properties including anti-inflammatory and hepatoprotective activities. However, the effect and possible mechanism of GLPS treatment on liver injury have not yet been reported. Therefore, this study aimed to explore the potential anti-inflammatory and hepatoprotective effects and possible mechanism of GLPS in carbon tetrachloride (CCl4)-induced acute liver injury mice. Summary: GLPS significantly reduced the activation of NLRP3 inflammasome and improved liver function in liver injury mice. It significantly inhibited CCl4-induced changes of alanine aminotransferase and aspartate aminotransferase activities in serum, as well as nitric oxide synthase (NOS) and cytochrome P450 2E1 (CYP2E1) activities in liver tissue; it also remarkably decreased levels of liver weight and index, total bilirubin, interleukin (IL)-1β, IL-18, IL-6 and tumor necrosis factor-α in serum, as well as malondialdehyde and IL-1β in liver tissue. Protein expression levels of liver NLRP3, ASC, and Caspase-1 were also downregulated, while the glutathione level in liver tissue was remarkably enhanced in GLPS groups compared to that of the model group. Key Message: These results suggested that GLPS may be a potential for the prevention and treatment of acute liver injury with liver inflammation. The possible mechanism may be related to the inhibition of free radical lipid peroxidation, NOS, and CYP2E1 activities and activation of liver inflammatory factors.

Download Full-text