scholarly journals Stain-less staining for computed histopathology

TECHNOLOGY ◽  
2015 ◽  
Vol 03 (01) ◽  
pp. 27-31 ◽  
Author(s):  
David Mayerich ◽  
Michael J. Walsh ◽  
Andre Kadjacsy-Balla ◽  
Partha S. Ray ◽  
Stephen M. Hewitt ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Clinical Practice ◽  
Clinical Pathology ◽  
Spectroscopic Imaging ◽  
Chemical Imaging ◽  
Tissue Composition ◽  
Alternative Approach ◽  
Ft Ir ◽  
Hematoxylin And Eosin ◽  
Ir Spectroscopic

Dyes such as hematoxylin and eosin (H&E) and immunohistochemical stains have been increasingly used to visualize tissue composition in research and clinical practice. We present an alternative approach to obtain the same information using stain-free chemical imaging. Relying on Fourier transform infrared (FT-IR) spectroscopic imaging and computation, stainless computed histopathology can enable a rapid, digital, quantitative and non-perturbing visualization of morphology and multiple molecular epitopes simultaneously in a variety of research and clinical pathology applications.

Protein hydration in living cells probed by Fourier transform infrared (FT-IR) spectroscopic imaging

The Analyst ◽  
2017 ◽  
Vol 142 (13) ◽  
pp. 2475-2483 ◽  
Author(s):  
H. Shinzawa ◽  
B. Turner ◽  
J. Mizukado ◽  
S. G. Kazarian
Keyword(s):  
Fourier Transform ◽  
Ir Spectra ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Protein Hydration ◽  
Ft Ir ◽  
Ir Spectroscopic

FT-IR spectra of a HEK cell were analyzed with 2D disrelation mapping to reveal molecular states of water and protein hydration.

Label-Free Diagnosis of Pulmonary Fat Embolism Using Fourier Transform Infrared (FT-IR) Spectroscopic Imaging

2022 ◽  
pp. 000370282110614
Author(s):  
Qi Cheng ◽  
Yongzheng Zhu ◽  
Kaifei Deng ◽  
Zhiqiang Qin ◽  
Jianhua Zhang ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Abdominal Wall ◽  
Lung Tissue ◽  
Lipid Droplets ◽  
Spectral Imaging ◽  
Spectroscopic Imaging ◽  
Fat Embolism ◽  
Infrared Spectral ◽  
Ft Ir ◽  
Ir Spectroscopic

The diagnosis of pulmonary fat embolism (PFE) is of great significance in the field of forensic medicine because it can be considered a major cause of death or a vital reaction. Conventional histological analysis of lung tissue specimens is a widely used method for PFE diagnosis. However, variable and labor-intensive tissue staining procedures impede the validity and informativeness of histological image analysis. To obtain complete information from tissues, a method based on infrared imaging of unlabeled tissue sections was developed to identify pulmonary fat emboli in the present study. We selected 15 PFE-positive lung samples and 15 PFE-negative samples from real cases. Oil red O (ORO) staining and infrared spectral imaging collection were both performed on all lung tissue samples. And the fatty tissue of the abdominal wall and the embolized lipid droplets in the lungs were taken for comparison. The results of the blind, evaluation by pathologists, showed good agreement between the infrared spectral imaging of the lung tissue and the standard histological stained images. Fourier transform infrared (FT-IR) spectroscopic imaging significantly simplifies the typical painstakingly laborious histological staining procedure. And we found a difference between lipid droplets embolized in abdominal wall fat and lung tissue.

Generalized Implementation of Rapid-Scan Fourier Transform Infrared Spectroscopic Imaging

2002 ◽  
Vol 56 (8) ◽  
pp. 965-969 ◽  
Author(s):  
Scott W. Huffman ◽  
Rohit Bhargava ◽  
Ira W. Levin
Keyword(s):  
Fourier Transform ◽  
Data Acquisition ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Imaging Data ◽  
Rapid Scan ◽  
Continuous Scanning ◽  
Ft Ir ◽  
Ir Instrumentation ◽  
Ir Spectroscopic

We describe a novel, generalized data acquisition sequence to allow rapid-scan Fourier transform infrared (FT-IR) spectroscopic imaging using focal plane array (FPA) detectors. This technique derives its applicability from the reproducible performance of modern FT-IR instrumentation and the availability of FPAs with simultaneous, full array acquisition, or snapshot electronics. Instead of sampling the entire interferogram in one mirror sweep over a predetermined retardation, as in traditional continuous-scanning techniques, the modulated light from the interferometer is recorded over several mirror sweeps. The FPA detector is synchronized for data acquisition after a specified delay with respect to the initiation of the mirror motion to provide a highly under-sampled interferogram. By incorporating appropriate delays in subsequent interferometer mirror scans, the entire interferogram is sampled and reconstructed. The signal-to-noise ratios (SNR) of the resulting interferograms are analyzed and are compared with step-scan spectroscopic imaging data.

scholarly journals Attenuated total reflection-FT-IR spectroscopic imaging of protein crystallization

2010 ◽  
Vol 66 (a1) ◽  
pp. s294-s295
Author(s):  
Naomi E. Chayen ◽  
Lata Govada ◽  
K. L. Andrew Chan ◽  
Roslyn M. Bill ◽  
Sergei G. Kazarian
Keyword(s):  
Protein Crystallization ◽  
Spectroscopic Imaging ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Ft Ir ◽  
Ir Spectroscopic
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