Generalized Implementation of Rapid-Scan Fourier Transform Infrared Spectroscopic Imaging

2002 ◽  
Vol 56 (8) ◽  
pp. 965-969 ◽  
Author(s):  
Scott W. Huffman ◽  
Rohit Bhargava ◽  
Ira W. Levin
Keyword(s):  
Fourier Transform ◽  
Data Acquisition ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Imaging Data ◽  
Rapid Scan ◽  
Continuous Scanning ◽  
Ft Ir ◽  
Ir Instrumentation ◽  
Ir Spectroscopic

We describe a novel, generalized data acquisition sequence to allow rapid-scan Fourier transform infrared (FT-IR) spectroscopic imaging using focal plane array (FPA) detectors. This technique derives its applicability from the reproducible performance of modern FT-IR instrumentation and the availability of FPAs with simultaneous, full array acquisition, or snapshot electronics. Instead of sampling the entire interferogram in one mirror sweep over a predetermined retardation, as in traditional continuous-scanning techniques, the modulated light from the interferometer is recorded over several mirror sweeps. The FPA detector is synchronized for data acquisition after a specified delay with respect to the initiation of the mirror motion to provide a highly under-sampled interferogram. By incorporating appropriate delays in subsequent interferometer mirror scans, the entire interferogram is sampled and reconstructed. The signal-to-noise ratios (SNR) of the resulting interferograms are analyzed and are compared with step-scan spectroscopic imaging data.

Protein hydration in living cells probed by Fourier transform infrared (FT-IR) spectroscopic imaging

The Analyst ◽  
2017 ◽  
Vol 142 (13) ◽  
pp. 2475-2483 ◽  
Author(s):  
H. Shinzawa ◽  
B. Turner ◽  
J. Mizukado ◽  
S. G. Kazarian
Keyword(s):  
Fourier Transform ◽  
Ir Spectra ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Protein Hydration ◽  
Ft Ir ◽  
Ir Spectroscopic

FT-IR spectra of a HEK cell were analyzed with 2D disrelation mapping to reveal molecular states of water and protein hydration.

Time-Resolved Fourier Transform Infrared Spectroscopic Imaging

2003 ◽  
Vol 57 (4) ◽  
pp. 357-366 ◽  
Author(s):  
Rohit Bhargava ◽  
Ira W. Levin
Keyword(s):  
Fourier Transform ◽  
Imaging Modality ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Dynamic Processes ◽  
Time Resolved ◽  
Ir Imaging ◽  
Infrared Spectroscopic ◽  
Infrared Spectroscopic Imaging ◽  
Ft Ir

Fourier transform infrared (FT-IR) imaging allows simultaneous spectral characterization of large spatial areas due to its multichannel detection advantage. The acquisition of large amounts of data in the multichannel configuration results, however, in a poor temporal resolution of sequentially acquired data sets, which limits the examination of dynamic processes to processes that have characteristic time scales of the order of minutes. Here, we introduce the concept and instrumental details of a time-resolved infrared spectroscopic imaging modality that permits the examination of repetitive dynamic processes whose half-lives are of the order of milliseconds. As an illustration of this implementation of step-scan FT-IR imaging, we examine the molecular responses to external electric-field perturbations of a microscopically heterogeneous polymer–liquid crystal composite. Analysis of the spectroscopic data using conventional univariate and generalized two-dimensional (2D) correlation methods emphasizes an additional capability for accessing of simultaneous spatial and temporal chemical measurements of molecular dynamic processes.

Identification of Large Isotope Anomalies in Quartz by Infrared Spectroscopy

2019 ◽  
Vol 73 (7) ◽  
pp. 767-773
Author(s):  
Ryan C. Ogliore ◽  
Cosette Dwyer ◽  
Michael J. Krawczynski ◽  
Hélène Couvy ◽  
Max Eisele ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Isotope Composition ◽  
Fourier Transform Infrared ◽  
Spectroscopic Technique ◽  
Far Field ◽  
Ft Ir ◽  
Ir Spectroscopic ◽  
Quartz Grains

We report an infrared (IR) spectroscopic technique to detect quartz grains with large isotope anomalies. We synthesized isotopically doped quartz and used Fourier transform infrared spectroscopy (FT-IR) in two different instruments: a traditional far-field instrument and a neaSpec nanoFT-IR, to quantify the shift in the peak of the Si–O stretch near 780 cm−1 as a function of isotope composition, and the uncertainty in this shift. From these measurements, we estimated the minimum detectable isotope anomaly using FT-IR. The described technique can be used to nondestructively detect very small (30 nm) presolar grains. In particular, supernova grains, which can have very large isotope anomalies, are detectable by this method.

scholarly journals Stain-less staining for computed histopathology

TECHNOLOGY ◽  
2015 ◽  
Vol 03 (01) ◽  
pp. 27-31 ◽  
Author(s):  
David Mayerich ◽  
Michael J. Walsh ◽  
Andre Kadjacsy-Balla ◽  
Partha S. Ray ◽  
Stephen M. Hewitt ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Clinical Practice ◽  
Clinical Pathology ◽  
Spectroscopic Imaging ◽  
Chemical Imaging ◽  
Tissue Composition ◽  
Alternative Approach ◽  
Ft Ir ◽  
Hematoxylin And Eosin ◽  
Ir Spectroscopic

Dyes such as hematoxylin and eosin (H&E) and immunohistochemical stains have been increasingly used to visualize tissue composition in research and clinical practice. We present an alternative approach to obtain the same information using stain-free chemical imaging. Relying on Fourier transform infrared (FT-IR) spectroscopic imaging and computation, stainless computed histopathology can enable a rapid, digital, quantitative and non-perturbing visualization of morphology and multiple molecular epitopes simultaneously in a variety of research and clinical pathology applications.

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