Visualizing horn evolution by morphing high-resolution X-ray CT images

Author(s):  
Wendy L. Hodges ◽  
Theodore Garland ◽  
Reuben Reyes ◽  
Timothy Rowe
Keyword(s):  
2013 ◽  
Vol 16 (04) ◽  
pp. 353-368 ◽  
Author(s):  
A.. Dehghan Khalili ◽  
J.-Y.. -Y. Arns ◽  
F.. Hussain ◽  
Y.. Cinar ◽  
W.V.. V. Pinczewski ◽  
...  

Summary High-resolution X-ray-computed-tomography (CT) images are increasingly used to numerically derive petrophysical properties of interest at the pore scale—in particular, effective permeability. Current micro-X-ray-CT facilities typically offer a resolution of a few microns per voxel, resulting in a field of view of approximately 5 mm3 for a 2,0482 charge-coupled device. At this scale, the resolution is normally sufficient to resolve pore-space connectivity and calculate transport properties directly. For samples exhibiting heterogeneity above the field of view of such a single high-resolution tomogram with resolved pore space, a second low-resolution tomogram can provide a larger-scale porosity map. This low-resolution X-ray-CT image provides the correlation structure of porosity at an intermediate scale, for which high-resolution permeability calculations can be carried out, forming the basis for upscaling methods dealing with correlated heterogeneity. In this study, we characterize spatial heterogeneity by use of overlapping registered X-ray-CT images derived at different resolutions spanning orders of magnitude in length scales. A 38-mm-diameter carbonate core is studied in detail and imaged at low resolution—and at high resolution by taking four 5-mm-diameter subsets, one of which is imaged by use of full-length helical scanning. Fine-scale permeability transforms are derived by use of direct porosity/permeability relationships, random sampling of the porosity/permeability scatter plot as a function of porosity, and structural correlations combined with stochastic simulation. A range of these methods is applied at the coarse scale. We compare various upscaling methods, including renormalization theory, with direct solutions by use of a Laplace solver and report error bounds. Finally, we compare with experimental measurements of permeability at both the small-plug and the full-plug scale. We find that both numerically and experimentally for the carbonate sample considered, which displays nonconnecting vugs and intrafossil pores, permeability increases with scale. Although numerical and experimental results agree at the larger scale, the digital core-analysis results underestimate experimentally measured permeability at the smaller scale. Upscaling techniques that use basic averaging techniques fail to provide truthful vertical permeability at the fine scale because of large permeability contrasts. At this scale, the most accurate upscaling technique uses Darcy's law. At the coarse scale, an accurate permeability estimate with error bounds is feasible if spatial correlations are considered. All upscaling techniques work satisfactorily at this scale. A key part of the study is the establishment of porosity transforms between high-resolution and low-resolution images to arrive at a calibrated porosity map to constrain permeability estimates for the whole core.


Author(s):  
Robert A. Grant ◽  
Laura L. Degn ◽  
Wah Chiu ◽  
John Robinson

Proteolytic digestion of the immunoglobulin IgG with papain cleaves the molecule into an antigen binding fragment, Fab, and a compliment binding fragment, Fc. Structures of intact immunoglobulin, Fab and Fc from various sources have been solved by X-ray crystallography. Rabbit Fc can be crystallized as thin platelets suitable for high resolution electron microscopy. The structure of rabbit Fc can be expected to be similar to the known structure of human Fc, making it an ideal specimen for comparing the X-ray and electron crystallographic techniques and for the application of the molecular replacement technique to electron crystallography. Thin protein crystals embedded in ice diffract to high resolution. A low resolution image of a frozen, hydrated crystal can be expected to have a better contrast than a glucose embedded crystal due to the larger density difference between protein and ice compared to protein and glucose. For these reasons we are using an ice embedding technique to prepare the rabbit Fc crystals for molecular structure analysis by electron microscopy.


Author(s):  
G. Van Tendeloo ◽  
J. Van Landuyt ◽  
S. Amelinckx

Polytypism has been studied for a number of years and a wide variety of stacking sequences has been detected and analysed. SiC is the prototype material in this respect; see e.g. Electron microscopy under high resolution conditions when combined with x-ray measurements is a very powerful technique to elucidate the correct stacking sequence or to study polytype transformations and deviations from the ideal stacking sequence.


Author(s):  
R. Gronsky

The phenomenon of clustering in Al-Ag alloys has been extensively studied since the early work of Guinierl, wherein the pre-precipitation state was characterized as an assembly of spherical, ordered, silver-rich G.P. zones. Subsequent x-ray and TEM investigations yielded results in general agreement with this model. However, serious discrepancies were later revealed by the detailed x-ray diffraction - based computer simulations of Gragg and Cohen, i.e., the silver-rich clusters were instead octahedral in shape and fully disordered, atleast below 170°C. The object of the present investigation is to examine directly the structural characteristics of G.P. zones in Al-Ag by high resolution transmission electron microscopy.


Author(s):  
Yun Lu ◽  
David C. Joy

High resolution scanning electron microscopy (SEM) and energy dispersive x-ray analysis (EDXA) were performed to investigate microparticles in blended cements and their hydration products containing sodium-rich chemical wastes. The physical appearance of powder particles and the morphological development at different hydration stages were characterized by using high resolution SEM Hitachi S-900 and by SEM S-800 with a EDX spectrometer. Microparticles were dispersed on the sample holder and glued by 1% palomino solution. Hydrated bulk samples were dehydrated by acetone and mounted on the holder by silver paste. Both fracture surfaces and flat cutting sections of hydrating samples were prepared and examined. Some specimens were coated with an 3 nm thick Au-Pd or Cr layer to provide good conducting surfaces. For high resolution SEM S-900 observations the accelerating voltage of electrons was 1-2 KeV to protect the electron charging. Microchemical analyses were carried out by S800/EDS equipped with a LINK detector of take-off angle =40°.


Author(s):  
K. H. Downing ◽  
S. G. Wolf ◽  
E. Nogales

Microtubules are involved in a host of critical cell activities, many of which involve transport of organelles through the cell. Different sets of microtubules appear to form during the cell cycle for different functions. Knowledge of the structure of tubulin will be necessary in order to understand the various functional mechanisms of microtubule assemble, disassembly, and interaction with other molecules, but tubulin has so far resisted crystallization for x-ray diffraction studies. Fortuitously, in the presence of zinc ions, tubulin also forms two-dimensional, crystalline sheets that are ideally suited for study by electron microscopy. We have refined procedures for forming the sheets and preparing them for EM, and have been able to obtain high-resolution structural data that sheds light on the formation and stabilization of microtubules, and even the interaction with a therapeutic drug.Tubulin sheets had been extensively studied in negative stain, demonstrating that the same protofilament structure was formed in the sheets and microtubules. For high resolution studies, we have found that the sheets embedded in either glucose or tannin diffract to around 3 Å.


Author(s):  
Y. Y. Wang ◽  
H. Zhang ◽  
V. P. Dravid ◽  
H. Zhang ◽  
L. D. Marks ◽  
...  

Azuma et al. observed planar defects in a high pressure synthesized infinitelayer compound (i.e. ACuO2 (A=cation)), which exhibits superconductivity at ~110 K. It was proposed that the defects are cation deficient and that the superconductivity in this material is related to the planar defects. In this report, we present quantitative analysis of the planar defects utilizing nanometer probe xray microanalysis, high resolution electron microscopy, and image simulation to determine the chemical composition and atomic structure of the planar defects. We propose an atomic structure model for the planar defects.Infinite-layer samples with the nominal chemical formula, (Sr1-xCax)yCuO2 (x=0.3; y=0.9,1.0,1.1), were prepared using solid state synthesized low pressure forms of (Sr1-xCax)CuO2 with additions of CuO or (Sr1-xCax)2CuO3, followed by a high pressure treatment.Quantitative x-ray microanalysis, with a 1 nm probe, was performed using a cold field emission gun TEM (Hitachi HF-2000) equipped with an Oxford Pentafet thin-window x-ray detector. The probe was positioned on the planar defects, which has a 0.74 nm width, and x-ray emission spectra from the defects were compared with those obtained from vicinity regions.


Author(s):  
W. Coene ◽  
F. Hakkens ◽  
T.H. Jacobs ◽  
K.H.J. Buschow

Intermetallic compounds of the type RE2Fe17Cx (RE= rare earth element) are promising candidates for permanent magnets. In case of Y2Fe17Cx, the Curie temperature increases from 325 K for x =0 to 550 K for x = 1.6 . X ray and electron diffraction reveal a carbon - induced structural transformation in Y2Fe17Cx from the hexagonal Th2Ni17 - type (x < 0.6 ) to the rhombohedral Th2Zn17 - type ( x ≥ 0.6). Planar crystal defects introduce local sheets of different magnetic anisotropy as compared with the ordered structure, and therefore may have an important impact on the coercivivity mechanism .High resolution electron microscopy ( HREM ) on a Philips CM30 / Super Twin has been used to characterize planar crystal defects in rhombohedral Y2Fe17Cx ( x ≥ 0.6 ). The basal plane stacking sequences are imaged in the [100] - orientation, showing an ABC or ACB sequence of Y - atoms and Fe2 - dumbbells, for both coaxial twin variants, respectively . Compounds resulting from a 3 - week annealing treatment at high temperature ( Ta = 1000 - 1100°C ) contain a high density of planar defects.


1978 ◽  
Vol 3 ◽  
pp. 357-357
Author(s):  
J. Als-Nielsen ◽  
R. J. Birgeneau ◽  
M. Kaplan ◽  
J. D. Litster ◽  
C. R. Safinya
Keyword(s):  
X Ray ◽  

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