Fabrication of Wound Dressing Cotton Nano-Composite Coated with Tragacanth/Polyvinyl Alcohol: Characterization and In Vitro Studies

Nano silver/polyvinyl alcohol hydrogel nanocomposites have been prepared using gamma irradiation technique. Gamma irradiation serves as a crosslinking agent for the polyvinyl alcohol hydrogels and also acts as a reducing agent for reduction of Ag+ ions to zero valent Ag0 within the polyvinyl alcohol crosslinked network. The microstructural characteristics of the prepared composites were studied using powder X-ray diffraction, ultraviolet–visible spectroscopy, Fourier-transform infrared spectroscopy and field emission scanning electron microscopy analysis. The data obtained by these characterizations indicate the homogeneous distribution of silver nanoparticles on the polyvinyl alcohol network. The swelling properties and mechanical parameters of the silver/polyvinyl alcohol hydrogel nanocomposites tend to show improvements, making them a better material for wound care applications. The silver/polyvinyl alcohol hydrogel nanocomposites have shown good antibacterial potential against both Gram-positive and Gram-negative bacteria and shown nil or minimal cytotoxic effect on human melanoma (SK-MEL-2) and mouse melanoma (B16-F1) cell lines. Overall, it was concluded that under optimized condition, silver/polyvinyl alcohol hydrogel nanocomposites synthesized using gamma irradiation technique are excellent candidates for wound dressing application.

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Erythropoietin/aloe vera-releasing wet-electrospun polyvinyl alcohol/chitosan sponge-like wound dressing: In vitro and in vivo studies

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911517731793 ◽

2017 ◽

Vol 33 (3) ◽

pp. 269-281 ◽

Cited By ~ 10

Author(s):

Mahdi Naseri-Nosar ◽

Saeed Farzamfar ◽

Majid Salehi ◽

Ahmad Vaez ◽

Roksana Tajerian ◽

...

Keyword(s):

Polyvinyl Alcohol ◽

Wound Dressing ◽

Aloe Vera ◽

In Vivo Studies

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Preclinical assessment of chitosan–polyvinyl alcohol–graphene oxide nanocomposite scaffolds as a wound dressing

Polymers and Polymer Composites ◽

10.1177/09673911211029242 ◽

2021 ◽

pp. 096739112110292

Author(s):

Arash Montazeri ◽

Fariba Saeedi ◽

Yaser Bahari ◽

Ahmad Ahmadi Daryakenari

Keyword(s):

Graphene Oxide ◽

Polyvinyl Alcohol ◽

Wound Dressing ◽

Biological Properties ◽

Mouse Fibroblast ◽

Wound Dressings ◽

In Vivo Studies ◽

Nanocomposite Scaffolds

The present research aimed to examine the biological properties of chitosan (CS)–polyvinyl alcohol (PVA) scaffolds reinforced with graphene oxide (GO) nanosheets, as wound dressings. The scaffolds were characterized by various techniques. The scanning electron microscopy (SEM) and thermogravimetry analyses (TGAs) were used to investigate distribution of the GO within the polymer. The viscoelastic properties were evaluated by dynamic mechanical thermal analysis (DMTA) to examine the quality of a wound dressing. In vitro and in vivo studies were conducted to assess the biocompatibility of the scaffolds as wound dressing. The cell viability and proliferation results indicated that mouse fibroblast cells (L929) could adhere on the 50CS–50PVA/3 wt% GO scaffold. Herewith, the fabricated CS–PVA–GO nanocomposite scaffolds are suggested as promising biomaterials for skin tissue engineering and wound dressing.

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A Novel Bilayer Propionyl-L-carnitine Loaded Polyvinyl Alcohol/Calcium Alginate/Carboxymethyl Cellulose wound dressing for the treatment of diabetic wounds: an in vitro and in vivo study

10.21203/rs.3.rs-217915/v1 ◽

2021 ◽

Author(s):

Wenjun Jiang ◽

Zuoyao Huang ◽

Weixiang Min ◽

Hao Zhang

Keyword(s):

Polyvinyl Alcohol ◽

Carboxymethyl Cellulose ◽

Wound Dressing ◽

Calcium Alginate ◽

Wound Dressings ◽

Vapor Permeability ◽

Drying Methods ◽

Cell Viability Assay ◽

Diabetic Wounds

Abstract In the current study a drug delivering bilayer porous/nanofibrous wound dressing was developed using a combination of electrospinning and freeze-drying methods. The wound dressings were prepared by lyophilization of 1:1 weight ratios of calcium alginate and carboxymethyl cellulose (CMC) solutions. Drug delivering nanofibrous sheets were fabricated by electrospinning of polyvinyl alcohol (PVA) solution incorporated with 1%,3%,5%, and 10% of Propionyl-L-carnitine. The dressings were studied regarding their microstructure, swelling capacity, mechanical strength, surface wettability, water vapor permeability, drug release profile, in vitro degradation, cell viability assay, hemocompatibility, porosity measurement, microbial penetration assay, and protein adsorption assay. Based on in vitro studies, PVA sheets loaded with 5% Propionyl-L-carnitine was chosen for the preparation of wound dressings. The healing potential of the produced constructs was studied in rat model of diabetic wound. Our results showed that the drug delivering dressings demonstrated significantly higher wound closure and better histological regeneration compared to drug free constructs and sterile gauze. Our results suggest potential applicability of Propionyl-L-carnitine delivering Calcium Alginate/CMC/PVA dressing for the treatment of diabetic wounds in clinic.

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Synthesis, characterization, and in vitro studies of graphene oxide/chitosan–polyvinyl alcohol films

Carbohydrate Polymers ◽

10.1016/j.carbpol.2013.10.085 ◽

2014 ◽

Vol 102 ◽

pp. 813-820 ◽

Cited By ~ 79

Author(s):

Andreea Madalina Pandele ◽

Mariana Ionita ◽

Livia Crica ◽

Sorina Dinescu ◽

Marieta Costache ◽

...

Keyword(s):

Graphene Oxide ◽

Polyvinyl Alcohol ◽

In Vitro Studies ◽

Polyvinyl Alcohol Films

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Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment

International Journal of Nanomedicine ◽

10.2147/ijn.s266692 ◽

2021 ◽

Vol Volume 16 ◽

pp. 223-235

Author(s):

Ensieh Ghasemian Lemraski ◽

Hossein Jahangirian ◽

Maryam Dashti ◽

Elaheh Khajehali ◽

Mis. Soheila Sharafinia ◽

...

Keyword(s):

Polyvinyl Alcohol ◽

Double Layer ◽

Wound Dressing ◽

In Vivo Assessment

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Nanocomposite sponges of sodium alginate/graphene oxide/polyvinyl alcohol as potential wound dressing: In vitro and in vivo evaluation

Composites Part B Engineering ◽

10.1016/j.compositesb.2019.03.006 ◽

2019 ◽

Vol 167 ◽

pp. 396-405 ◽

Cited By ~ 94

Author(s):

Rongxiu Ma ◽

Yajing Wang ◽

Houjuan Qi ◽

Cai Shi ◽

Guangbiao Wei ◽

...

Keyword(s):

Graphene Oxide ◽

Polyvinyl Alcohol ◽

Sodium Alginate ◽

Wound Dressing ◽

In Vivo Evaluation

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Fabricated approach for an effective wound dressing material based on a natural gum impregnated with Acalypha indica extract

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911518801046 ◽

2018 ◽

Vol 33 (6) ◽

pp. 612-628 ◽

Cited By ~ 1

Author(s):

Preethi Jenifer ◽

Mangathayaru Kalachaveedu ◽

Arun Viswanathan ◽

Arumugam Gnanamani ◽

Mubeena

Keyword(s):

Cell Proliferation ◽

Polyvinyl Alcohol ◽

Wound Dressing ◽

Guar Gum ◽

Three Dimensional ◽

Dermal Fibroblasts ◽

Raw Material ◽

Cell Culture Study ◽

Acalypha Indica

Although acute wounds are common, treatment for a scarless condition remains limited and ineffective as medicated dressings act only as an epidermal coverage and no interdermal interactions happen. This study examined the benefit of Acalypha indica, a traditionally acclaimed plant for wound healing, as a three-dimensional nanofibrous dressing. guar gum, a natural polysaccharide, was chosen as the raw material, in combination with a synthetic copolymer polyvinyl alcohol. A series of polymer blend nanofibers made of 3:7 of 1% wt guar and 10% wt polyvinyl alcohol along with varied ratios of A. indica were prepared using electrospinning. The effect of cross-linking by citric acid on the nanofibers was studied using Fourier transform infrared. A 5% wt A. indica content was optimized in the electrospun solution to get nanosized morphology, roughness, water absorbing capacity, thermal stability, and tensile strength. The composite material was found inhibitory to both Gram-positive and Gram-negative strains as measured by zone of inhibition. The ability of the dressing to support and proliferate human dermal fibroblasts was evaluated by cell proliferation assay and Calcein acetomethyl (AM) staining assay. The results confirmed that the composite dressing could support long-term cell growth in the 9-day cell culture study. The incorporation of A. indica to guar/polyvinyl alcohol composite nanofibers has thus resulted in a wound dressing material with nanosized morphology for mimicking the extracellular matrix, surface roughness for the absorbtion of proteins, swelling capacity for the absorption of exudates, antimicrobial activity for prevention of microbial infections, and cell proliferation activity for the complete wound closure. The electrospun guar/polyvinyl alcohol/ A. indica composite which proved to be a suitable dressing for acute wounds when ascertained in vitro can surely be developed as an ethical plant bioactive wound healant.

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