A Low-Cost Aptamer Immobilization Technique for the Selective Detection of Protein Biomarkers Using Electrochemical Impedance Spectroscopy

2017 ◽  
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Selective Detection ◽  
Protein Biomarkers

scholarly journals The influence of chromium as a diffusive additive in the boronizing treatment of AISI 4140 steel on the corrosion resistance of the coating evaluated by Electrochemical Impedance Spectroscopy (EIS)

2020 ◽  
Vol 318 ◽  
pp. 01040
Author(s):  
Dimitrios I. Zagkliveris ◽  
Azarias Mavropoulos ◽  
Efstathios Ntovinos ◽  
Georgios K. Triantafyllidis
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Industrial Applications ◽  
Pack Cementation ◽  
Boride Phase ◽  
Corrosion Resistant ◽  
Aisi 4140 Steel ◽  
Aisi 4140

A large variety of protective coating is being used in industrial applications to improve the resistance of the metallic substrates against corrosion. The pack-cementation method for boronizing and borochromizing is effective to produce extremely hard and corrosion resistant thick coatings and, additionally, is a low-cost and simple technique. In the present study, AISI 4140 steel specimens underwent boronizing and afterwards chromizing by the pack-cementation method using B4C as boron source and Fe-Cr as chromium source, respectively. In both treatments the appropriate activators were used. After chromizing the boronized substrate, a mixed boride phase FeCrB was formed, as it was confirmed by X-ray Diffractometry (XRD). The boronized and the borochromized specimens were subjected to Electrochemical Impedance Spectroscopy (EIS). From the analysis of the frequency response of the coating systems (Bode and Nyquist display), the conclusion that the borochromized specimens were significantly more corrosion resistant was extracted. Finally, data of optical and electron microscopy contribute to the validity of the conclusions.

Design of Automatic Measurement System of Lithium Battery Electrochemical Impedance Spectroscopy Based on Microcomputer

2012 ◽  
Vol 241-244 ◽  
pp. 259-264 ◽  
Author(s):  
Wang Li ◽  
Gen Wang Liu ◽  
Fu He Yang
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
High Precision ◽  
Measurement System ◽  
Lithium Battery ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Automatic Measurement ◽  
Response Curves ◽  
High Level

A system of miniaturized lithium battery electrochemical impedance spectroscopy (EIS) measurement is designed with high precision impedance converter chip AD5933 as its core. The measurement range of the system is from 0.010Hz to 100 KHz. Meanwhile, by using a high-level programming language of C#, an interface is developed which can real-time graphic display of EIS information. Through measurement and analysis of two types of impedance, the results show that detection precision of the system is less than 3.5%. Finally, amplitude-frequency response curves and Nyquist plots of HL-18650 M lithium battery at different state of charge (SOC) levels are measured. Compared with lithium battery EIS measurement system by traditional division, this system has the outstanding advantages of small size, high level of integration, low cost, simple operation and high precision. It is helpful to the mass production and application of lithium battery EIS measurement system.

scholarly journals Electrochemical Impedance Spectroscopy Characterization of Label-Free Biosensors for the Detection of HER2 in Saliva

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 17
Author(s):  
Imad Abrao Nemeir ◽  
Lynn Mouawad ◽  
Joseph Saab ◽  
Walid Hleihel ◽  
Abdelhamid Errachid ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Ease Of Use ◽  
Detection Methods ◽  
Cancer Type ◽  
Label Free ◽  
Human Epidermal Receptor

Breast cancer is the leading cancer type for women with two million new yearly infections and more than half a million dead worldwide. Human Epidermal Receptor 2 (HER2) is a prominent breast cancer biomarker that indicates aggressive cancer and is often associated with a bad prognosis and low survival rates. However, current detection methods for HER2 are often time-consuming, expensive, and require a high level of expertise. Biosensors are devices that turn biological interaction into a readable electronic signal; they are known for their high specificity and selectivity for low concentration, as well as their low cost and ease of use, thus making them a better alternative to traditional methods. Also, saliva is becoming a better alternative to blood for the detection of biomarkers due to its non-invasive collection in large quantities with simple collection methods with a richness in disease biomarkers including HER2. Thus, this project aims to develop a label-free, low cost, electrochemical biosensor for the detection of HER2 in saliva. This was done by first depositing diazonium salt onto a screen-printed electrode (SPE) through cyclic voltammetry, then immobilizing anti-HER2 antibodies on the activated SPE using 1-ethyl-3-(3-dimethylamino) propyl carbodiimide/N-hydroxysuccinimide. HER2 biomarker concentrations were detected using electrochemical impedance spectroscopy inside a microfluidic system. The biosensor showed a higher linear detection of HER2 (Y = 0.0062X + 0.1066/R2 = 0.9909) in its physiological concentration range of 5 and 40 pg/mL when compared to other interference proteins: Epidermal Growth Factor Receptor (Y = 0.0016X + 0.0188/R2 = 0.8072) and Human Epidermal Receptor 3 (Y = (0.0035X + 0.0225/R2 = 0.1302). The biosensor was then used to detect 10 pg/mL of HER2 concentration in real saliva using the standard addition methods (Y = 0.0118X + 0.1282/R2 = 0.9876).

A low-cost and miniaturized potentiostat for sensing of biomolecular species such as TNF-α by electrochemical impedance spectroscopy

2018 ◽  
Vol 100 ◽  
pp. 533-540 ◽  
Author(s):  
Raquel Pruna ◽  
Francisco Palacio ◽  
Abdoullatif Baraket ◽  
Nadia Zine ◽  
Angelos Streklas ◽  
...  
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Tnf Α

scholarly journals Digital Impedance Emulator for Battery Measurement System Calibration

Sensors ◽  
2021 ◽  
Vol 21 (21) ◽  
pp. 7377
Author(s):  
Francesco Santoni ◽  
Alessio De Angelis ◽  
Antonio Moschitta ◽  
Paolo Carbone
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Frequency Domain ◽  
Management System ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Battery Management System ◽  
Battery Management ◽  
Aging Effects ◽  
Custom Made

Meaningful information on the internal state of a battery can be derived by measuring its impedance. Accordingly, battery management systems based on electrochemical impedance spectroscopy are now recognized as a feasible solutions for online battery control and diagnostic. Since the impedance of a battery is always changing along with its state of charge and aging effects, it is important to have a stable impedance reference in order to calibrate and test a battery management system. In this work we propose a programmable impedance emulator that in principle could be used for the calibration of any battery management system based on electrochemical impedance spectroscopy. A digital finite-impulse-response filter is implemented, whose frequency response is programmed so as to reproduce exactly the impedance of a real battery in the frequency domain. The whole design process of the filter is presented in detail. An analytical expression for the impedance of real battery in the frequency domain is derived from an equivalent circuit model. The model is validated both through numerical simulations and experimental tests. In particular, the filter is implemented on a low-cost microcontroller unit, and the emulated impedance is measured by means of a custom-made electrochemical impedance spectroscopy measuring system, and verified by using standard commercial bench instruments. Results on this prototype show the feasibility of using the proposed emulator as a fully controllable and low-cost reference for calibrating battery impedance measurement systems.

Rapid analytical instrumentation for electrochemical impedance spectroscopy measurements

2019 ◽  
Author(s):  
yuan hu ◽  
Sean Brahim ◽  
Stefan Maat ◽  
Patricia Davies ◽  
Arpan Kundu ◽  
...  
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Wide Frequency Range ◽  
Measurement Time ◽  
Frequency Bands ◽  
Commercial Instrument ◽  
Adaptive Measurement ◽  
Eis Measurements

This work reports a low-cost custom electrochemical instrument capable of performing rapid and accurate electrochemical impedance spectroscopy (EIS) for supercapacitors over a broad frequency band (10 mHz to 2 kHz). Conventionally, EIS is measured via sinusoidal perturbations; however, such an approach suffers from lengthy measurement time. Chirp signals have been shown previously to reduce EIS measurement time for supercapacitors for relative narrow frequency bands (1 Hz to 2 kHz). However, to characterize supercapacitors comprehensively, much broader frequency bands are required. Here, we present a custom instrument with an adaptive measurement algorithm for performing EIS measurements in a wide frequency range of 10 mHz to 2 kHz with low measurement uncertainties. The results obtained using this new technique has been validated here with a commercial instrument on several types of supercapacitors. Furthermore, measurement time on average decreases from 1500 s to less than 400 s. The overall cost of the custom instrument is 90% lower as compared to the commercial instrument. The custom instrument's accuracy, time efficiency and low cost are expected to benefit electrochemical researchers.

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