Parathyroid gland volume increases with postmaturational  aging in the rat

To examine the pathophysiology of the age-related rise in the plasma concentration of parathyroid hormone (PTH), we studied the relationships among plasma immunoreactive PTH (iPTH), parathyroid gland volume, parathyroid cell proliferation rate, renal function, and blood Ca2+ in male Fischer 344 rats aged 6–28 mo. Plasma iPTH increased 2.5-fold between 6 and 28 mo and correlated with parathyroid gland volume ( r = 0.87). Gland volume began to increase as early as 6–12 mo of age and by 28 mo was threefold greater than at 6 mo. Gland expansion was a consequence of hyperplasia stimulated in part by an increase in cell proliferative activity late in life. Blood Ca2+ and plasma inorganic phosphorus did not change significantly with age. Glomerular filtration rate decreased with age but only after the age of 24 mo. Unlike what has been observed in the human, these data suggest that the age-related increase in plasma iPTH in the rat is linked to parathyroid gland hyperplasia and that early gland growth does not appear to be associated with hypocalcemia or renal insufficiency, but rather to developmentally related metabolic changes. Later in life (>24 mo), the increase in parathyroid cell proliferation rate, further hyperplastic expansion of the gland, and increase in iPTH secretion appear to be associated with renal insufficiency.

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Evidence for a Negative Correlation between Human Reactive Enamine-Imine Intermediate Deaminase A (RIDA) Activity and Cell Proliferation Rate: Role of Lysine Succinylation of RIDA

International Journal of Molecular Sciences ◽

10.3390/ijms22083804 ◽

2021 ◽

Vol 22 (8) ◽

pp. 3804

Author(s):

Luisa Siculella ◽

Laura Giannotti ◽

Benedetta Di Chiara Stanca ◽

Matteo Calcagnile ◽

Alessio Rochira ◽

...

Keyword(s):

Cell Proliferation ◽

Cell Lines ◽

Negative Correlation ◽

Cancer Biology ◽

Human Tumor ◽

In Silico Analysis ◽

Proliferation Rate ◽

Reactive Intermediate ◽

Cell Proliferation Rate

Reactive intermediate deaminase (Rid) proteins are enzymes conserved in all domains of life. UK114, a mammalian member of RidA subfamily, has been firstly identified as a component of liver perchloric acid-soluble proteins (L-PSP). Although still poorly defined, several functions have been attributed to the mammalian protein UK114/RIDA, including the reactive intermediate deamination activity. The expression of UK114/RIDA has been observed in some tumors, arousing interest in this protein as an evaluable tumor marker. However, other studies reported a negative correlation between UK114/RIDA expression, tumor differentiation degree and cell proliferation. This work addressed the question of UK114/RIDA expression in human non-tumor HEK293 cell lines and in some human tumor cell lines. Here we reported that human RIDA (hRIDA) was expressed in all the analyzed cell line and subjected to lysine (K-)succinylation. In HEK293, hRIDA K-succinylation was negatively correlated to the cell proliferation rate and was under the control of SIRT5. Moreover, K-succinylation clearly altered hRIDA quantification by immunoblotting, explaining, at least in part, some discrepancies about RIDA expression reported in previous studies. We found that hRIDA was able to deaminate reactive enamine-imine intermediates and that K-succinylation drastically reduced deaminase activity. As predicted by in silico analysis, the observed reduction of deaminase activity has been related to the drastic alterations of hRIDA structure inferred by K-succinylation. The role of hRIDA and the importance of its K-succinylation in cell metabolism, especially in cancer biology, have been discussed.

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Cell proliferation rate and nuclear morphometry in Roberts syndrome

Clinical Genetics ◽

10.1111/j.1399-0004.1998.tb03772.x ◽

2008 ◽

Vol 54 (6) ◽

pp. 512-516 ◽

Cited By ~ 6

Author(s):

Petros M Pavlopoulos ◽

Anastasia E Konstantinidou ◽

Emmanuel Agapitos ◽

Panagiotis Davaris

Keyword(s):

Cell Proliferation ◽

Proliferation Rate ◽

Roberts Syndrome ◽

Nuclear Morphometry ◽

Cell Proliferation Rate

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Analysis of cell proliferation rate in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Journal of Clinical and Experimental Dentistry ◽

10.4317/jced.2.e173 ◽

2010 ◽

pp. e173-177 ◽

Cited By ~ 2

Author(s):

S. Mehkri ◽

AR. Iyengar ◽

KS. Nagesh ◽

MB. Bharati

Keyword(s):

Cell Proliferation ◽

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Proliferation Rate ◽

Oral Leukoplakia ◽

Cell Proliferation Rate

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The Novel Long Noncoding RNA TUSC7 Inhibits Proliferation by Sponging MiR-211 in Colorectal Cancer

Cellular Physiology and Biochemistry ◽

10.1159/000457938 ◽

2017 ◽

Vol 41 (2) ◽

pp. 635-644 ◽

Cited By ~ 57

Author(s):

Jian Xu ◽

Rui Zhang ◽

Jian Zhao

Keyword(s):

Colorectal Cancer ◽

Cell Proliferation ◽

Tumor Suppressor ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Proliferation Rate ◽

Expression Level ◽

The Novel ◽

Cell Proliferation Rate ◽

Potential Tumor Suppressor

Background/Aims: The novel long noncoding RNA (lncRNA) tumor suppressor candidate 7 (TUSC7) has been reported as a potential tumor suppressor, while the functional role of TUSC7 is still unknown in colorectal cancer (CRC). Here, we characterized TUSC7 expression profile in CRC patients and investigated its biological function and potential molecular mechanism. Methods: RNA isolation, qRT-PCR, cell counter kit-8 assay, cell cycle assay, EdU assay, and western blot were performed. Statistical analyses were performed using SPSS 18.0 software and p value < 0.05 was considered as statistically significant. Results: In a cohort of CRC patients, we found TUSC7 was significantly downregulated in CRC tissues compared with adjacent non-tumor tissues (P < 0.01). Patients with high expression of TUSC7 had better survival than those with low expression of TUSC7 (HR = 0.342, 95% CI: 0.120-0.972, P = 0.044). Cell count kit 8 and EdU assays showed that ectopic expression of TUSC7 in HCT116 and SW480 cells significantly inhibited cell proliferation rate. After silence of TUSC7 with small interfering RNA, cell proliferation rate increased. Flow cytometry analyses revealed cycles were arrested at G1 phase after TUSC7 overexpression. We found there were 2 binding sites of miR-211-3p within the sequence of TUSC7 and TUSC7 expression level was negatively correlated with miR-211-3p. TUSC7 overexpression increased the expression level of CDK6, which is a downstream target of miR-211-3p, in both RNA and protein level. Furthermore, luciferase reporter assay indicated that TUSC7 could sponge miR-211-3p. Conclusion: To summary, we demonstrated that TUSC7 is a potential tumor suppressor in CRC, and TUSC7 could inhibit CRC cell proliferation by completely sponging miR-211-3p.

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Temperature induces variations in the retinal cell proliferation rate in a cyprinid

Brain Research ◽

10.1016/s0006-8993(01)02804-9 ◽

2001 ◽

Vol 913 (2) ◽

pp. 190-194 ◽

Cited By ~ 7

Author(s):

Almudena Velasco ◽

Elena Cid ◽

Juana Ciudad ◽

Alberto Orfao ◽

Jose Aijon ◽

...

Keyword(s):

Cell Proliferation ◽

Proliferation Rate ◽

Retinal Cell ◽

Cell Proliferation Rate

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R403 – Epidermal Proliferating Cell Nuclear Antigen in Aging Rats

Otolaryngology ◽

10.1016/j.otohns.2008.05.556 ◽

2008 ◽

Vol 139 (2_suppl) ◽

pp. P178-P178 ◽

Cited By ~ 3

Author(s):

Tapan K Bhattacharyya ◽

Paula Jackson ◽

J Regan Thomas

Keyword(s):

Cell Proliferation ◽

Proliferating Cell Nuclear Antigen ◽

Nuclear Antigen ◽

Proliferation Index ◽

Fischer 344 Rats ◽

Fischer 344 ◽

Age Related ◽

Two Parameters ◽

Proliferating Cell ◽

Effect Of Age

Problem To determine if epidermal cell proliferation in colony-raised Fischer 344 rats changes with age and diet. Methods Fischer 344 rats fed ad libitum and calorie-restricted (CR) diets were obtained from the NIA colonies, and young, young adult, and old animals from both groups were used for this study (six in each group). Tissue sections from the dorsal skin (DS) and foot plate (FP) were used for immunohistochemical staining of proliferating cell nuclear antigen (PCNA). The proliferation index (PCNA-I) was computed from counts of stained and total number of keratinocytes. Simultaneous measurements of epidermal thickness were obtained from same sections. Data were analyzed with Excel and SPSS 14.0 software for statistics. Results Two-way analysis of variance (ANOVA) was applied to the data to probe the effect of age, diet, and age-diet interaction. A significant effect of age was noticed in the two parameters i.e., DS PCNA (F 3.96, P .011), FP epidermal width (F 3.37, P .021) and FP PCNA-I (F 9.0, P .000). A significant correlation between DS width and PCNA values was also noted (r 0.5, P .01). Conclusion There is a trend of reduction of PCNA positive cells with increasing age irrespective of thickness of epidermis, and this trend is more apparent in CR rats. Significance This cell proliferation study has implications in relation to CR effect on age-related disease conditions, and biogerontology. Support The study was partially funded by the 2007 Leslie Bernstein grant from AAFPRS foundation.

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