Effect of adrenal steroids on bone resorption in rats

1976 ◽  
Vol 230 (1) ◽  
pp. 90-93 ◽  
Author(s):  
S Yasumura
Keyword(s):  
Bone Density ◽  
Bone Resorption ◽  
Serum Calcium ◽  
Adrenal Steroids ◽  
Calcium Diet ◽  
Inhibit Bone Resorption ◽  
Low Calcium Diet ◽  
Low Calcium ◽  
Radiographic Technique ◽  
Parathyroid Extract

Rats labeled with strontium-85 (85Sr) were rejected with adrenocortical steroids for 2 wk. The urinary-to-tibial (U/T) 85Sr ratio was used as an index of bone resorption. The glucocorticoids caused an inhibition of skeletal resorption, as judged by the 50% reduction in the U/T ratio, and decreased excretion of hydroxyproline. Thyroidal calcitonin levels were slightly elevated in glucocorticoid-treated animals, suggestive of a possible retardation of calcitonin release. The U/T ratios of thyroparathyroidectomized (TPTX) rats injected with corticosteroids were 50% of control values. The results indicate that glucocorticoids inhibit bone resorption independent of the action of calcitonin. Cortisol treatment increased the tibial density as measured by a radiographic technique. However, bone density was decreased and the U/T ratio increased in steroid-treated rats fed a low-calcium diet. In TPTX cortisol-treated rats, parathyroid extract (PTE) increased the U/T ratio and serum calcium but not to the degree observed in TPTX PTE-injected control animals. These experiments indicate that in rats glucocorticoids inhibit the rate of bone resorption but this effect can be overcome in part by PTE.

Effect of bisphosphonates on the increase in bone resorption induced by a low calcium diet

1996 ◽  
Vol 58 (6) ◽  
pp. 443-448 ◽  
Author(s):  
V. N. Antic ◽  
H. Fleisch ◽  
R. C. Mühlbauer
Keyword(s):  
Bone Resorption ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

scholarly journals Bone density observations in adolescents consuming a low calcium diet

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Kara A Egan ◽  
Berdine R Martin ◽  
George P McCabe ◽  
Stuart J Warden ◽  
Linda D McCabe ◽  
...  
Keyword(s):  
Bone Density ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

Effect of low calcium diet on bone resorption in bisphosphonate (BP) treated rats

1992 ◽  
Vol 17 ◽  
pp. S11
Author(s):  
V.N. Antic ◽  
R.C. Mühlbauer ◽  
H. Fleisch
Keyword(s):  
Bone Resorption ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

scholarly journals 318 Total flavonoids from Rhizoma drynariae ameliorate low calcium-induced osteoporosis by regulating the bone metabolism in caged laying hens

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 6-7
Author(s):  
Jie Huang ◽  
Lei Zhang ◽  
Zhongxin Zhou
Keyword(s):  
Bone Density ◽  
Laying Hens ◽  
Test Group ◽  
Economic Losses ◽  
Control Group ◽  
Total Flavonoids ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium ◽  
Rhizoma Drynariae

Abstract Cage layer osteoporosis (CLO) is one of the most serious bone metabolic diseases in laying hens industry. This disease not only causes huge economic losses, but also greatly affects the welfare of animals. Traditional Chinese medicines are widely used for prevention and treatment of all kinds of diseases. It is famous for excellent treatment, low side effects, and wide range of use safety. Total flavonoids from Rhizoma drynariae (TFRD) is an herbal product extracted from the dried root of Rhizoma drynariae. Recently, many researcher reports about the beneficial effects of TFRD in relation to various types of bone conditions but no report is available about promoting effect of TFRD against CLO. Therefore, current study was conducted to explore the ameliorating effect of TFRD in low calcium-induced osteoporosis. A total of 144 Lohmann pink-shell laying hens of 31-week-old were equally distributed in four groups: The control group (containing calcium 3.5% in based diet), the low calcium group (containing calcium 1.5% in low-calcium diet), the test group 1 (adding 500 mg/kg TFRD to low-calcium diet), the test group 2 (adding 2000 mg/kg TFRD to low-calcium diet). The birds were fed the diets for 60 d. The bone mineral density of tibia was measured by dual-energy X-ray absorptiometry indicating that the low calcium group had lower bone density than the control group. Compared with the low calcium group, the supplementation of TFRD protects bone tissue and prevents bone density from decreasing. Serum biochemical analysis showed that TFRD supplementation decreased the level of alkaline phosphatase, tartrate-resistant acid phosphatase, osteocalcin and calcitonin. Therefore, adding TFRD to low-calcium diet can improve the activity of important biochemical markers, clearly inhibit bone resorption and alleviate osteoporosis induce by low-calcium diet in caged laying hens.

Effect of low calcium diet on bone resorption in bisphosphonate (BP) treated rats

1992 ◽  
Vol 17 ◽  
pp. 229
Author(s):  
V.N. Antic ◽  
R.C. Mühlbauer ◽  
H. Fleisch
Keyword(s):  
Bone Resorption ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

Effect of Bisphosphonates on the Increase in Bone Resorption Induced by a Low Calcium Diet

1996 ◽  
Vol 58 (6) ◽  
pp. 443-448 ◽  
Author(s):  
V. N. Antic ◽  
H. Fleisch ◽  
R. C. Mühlbauer
Keyword(s):  
Bone Resorption ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

scholarly journals The beneficial effect of the sap of Acer mono in an animal with low-calcium diet-induced osteoporosis-like symptoms

2008 ◽  
Vol 100 (5) ◽  
pp. 1011-1018 ◽  
Author(s):  
Geun-Shik Lee ◽  
Hyuk-Soo Byun ◽  
Man-Hee Kim ◽  
Bo-Mi Lee ◽  
Sang-Hwan Ko ◽  
...  
Keyword(s):  
Serum Calcium ◽  
Calcium Concentration ◽  
Negative Control ◽  
Spring Water ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium ◽  
High Calcium ◽  
Femur Density ◽  
Acer Mono

The sap of Acer mono has been called ‘bone-benefit-water’ in Korea because of its mineral and sugar content. In particular, the calcium concentration of the sap of A. mono is 37·5 times higher than commercial spring water. In the current study, we examined whether A. mono sap could improve or prevent osteoporosis-like symptoms in a mouse model. Male mice (3 weeks old) were fed a low-calcium diet supplemented with 25, 50 or 100 % A. mono sap, commercial spring water or a high calcium-containing solution as a beverage for 7 weeks. There were no differences in weekly weight gain and food intake among all the groups. Mice that were given a low-calcium diet supplemented with commercial spring water developed osteoporosis-like symptoms. To assess the effect of sap on osteoporosis-like symptoms, we examined serum calcium concentration, and femur density and length, and carried out a histological examination. Serum calcium levels were significantly lower in mice that received a low-calcium diet supplemented with commercial spring water (the negative control group), and in the 25 % sap group compared to mice fed a normal diet, but were normal in the 50 and 100 % sap and high-calcium solution groups. Femur density and length were significantly reduced in the negative control and 25 % sap groups. These results indicate that a 50 % sap solution can mitigate osteoporosis-like symptoms induced by a low-calcium diet. We also examined the regulation of expression of calcium-processing genes in the duodenum and kidney. Duodenal TRPV6 and renal calbindin-D9k were up-regulated dose-dependently by sap, and the levels of these factors were higher than those attained in the spring water-treated control. The results demonstrate that the sap of A. mono ameliorates the low bone density induced by a low-calcium diet, most likely by increasing calcium ion absorption.

EFFECT OF CALCITONIN ON [3H]PROLINE INCORPORATION INTO BONE HYDROXYPROLINE IN THE RAT

1971 ◽  
Vol 49 (2) ◽  
pp. 233-241 ◽  
Author(s):  
D. N. KALU ◽  
G. V. FOSTER
Keyword(s):  
Daily Treatment ◽  
Growing Rats ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium ◽  
Proline Incorporation ◽  
Parathyroid Extract

SUMMARY The effect of calcitonin on the incorporation of [3H]proline into bone hydroxyproline was investigated in young growing rats. The action of the hormone was studied under the following conditions: (1) acutely, in both intact and thyroparathyroidectomized rats, (2) after 20 days of daily treatment in both intact and thyroparathyroidectomized animals, (3) in thyroparathyroidectomized animals treated with parathyroid extract for 20 days, and (4) in animals fed a low calcium diet for 25 days. Calcitonin had no effect when given either acutely or long-term to intact animals. [3H]Proline incorporation into bone hydroxyproline was increased in rats fed low calcium diet, in rats treated with parathyroid extract for 20 days, and in acutely thyroparathyroidectomized rats. In each instance, calcitonin prevented or reduced this increase. The results are interpreted as evidence that, with the doses used, hydroxyproline synthesis under normal conditions is unaffected by the hormone. However, where hydroxyproline formation was increased, calcitonin reduced this increase.

scholarly journals Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Zhengwang Yu ◽  
Jie Huang ◽  
Zhongxin Zhou
Keyword(s):  
Metabolic Diseases ◽  
Chinese Herb ◽  
Therapeutic Effects ◽  
Mineral Density ◽  
Steroid Biosynthesis ◽  
Metabolic Pathway Analysis ◽  
Effective Prevention ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

AbstractCage layer osteoporosis (CLO) is a common bone metabolism disease in the breeding industry of China. However, effective prevention for CLO has not been developed. Icariin (ICA), the main bioactive component of the Chinese herb Epimedium, has been shown to have good therapeutic effects on bone-related diseases. In this study, the effects of ICA were further evaluated in a low-calcium diet-induced CLO, and a serum metabolomics assay was performed to understand the underlying mechanisms. A total of 144 31-wk-old Lohmann pink-shell laying hens were randomly allocated to 4 groups with 6 replicates of 6 hens per replicate. The 4 dietary treatment groups consisted of a basal diet (3.5% calcium), a low-calcium diet (2.0% calcium), and a low-calcium diet supplemented with 0.5 or 2.0 g/kg ICA. The results showed that ICA exerted good osteoprotective effects on low-calcium diet-induced CLO. ICA significantly increased femur bone mineral density, improved bone microstructure, decreased bone metabolic level, and upregulated mRNA expression of bone formation genes in femoral bone tissue. Serum untargeted metabolomics analysis showed that 8 metabolite levels were significantly changed after ICA treatment, including increased contents of 7-dehydrocholesterol, 7-oxocholesterol, desmosterol, PC (18:1(9Z)/18:1(9Z)), PS (18:0/18:1(9Z)), N,N-dimethylaniline and 2-hydroxy-butanoic acid and decreased N2,N2-dimethylguanosine. Metabolic pathway analysis based on the above 8 metabolites indicated that ICA mainly perturbed steroid biosynthesis and glycerophospholipid metabolism. These findings suggest that ICA can effectively prevent bone loss in low-calcium diet-induced CLO by mediating steroid biosynthesis and glycerophospholipid metabolism and provide new information for the regulation of bone metabolic diseases.

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