EFFECT OF CALCITONIN ON [3H]PROLINE INCORPORATION INTO BONE HYDROXYPROLINE IN THE RAT

1971 ◽  
Vol 49 (2) ◽  
pp. 233-241 ◽  
Author(s):  
D. N. KALU ◽  
G. V. FOSTER
Keyword(s):  
Daily Treatment ◽  
Growing Rats ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium ◽  
Proline Incorporation ◽  
Parathyroid Extract

SUMMARY The effect of calcitonin on the incorporation of [3H]proline into bone hydroxyproline was investigated in young growing rats. The action of the hormone was studied under the following conditions: (1) acutely, in both intact and thyroparathyroidectomized rats, (2) after 20 days of daily treatment in both intact and thyroparathyroidectomized animals, (3) in thyroparathyroidectomized animals treated with parathyroid extract for 20 days, and (4) in animals fed a low calcium diet for 25 days. Calcitonin had no effect when given either acutely or long-term to intact animals. [3H]Proline incorporation into bone hydroxyproline was increased in rats fed low calcium diet, in rats treated with parathyroid extract for 20 days, and in acutely thyroparathyroidectomized rats. In each instance, calcitonin prevented or reduced this increase. The results are interpreted as evidence that, with the doses used, hydroxyproline synthesis under normal conditions is unaffected by the hormone. However, where hydroxyproline formation was increased, calcitonin reduced this increase.

scholarly journals The effects of long-term low-dose EHDP on experimental osteoporosis induced by ovariectomy and low calcium diet in rats.

1984 ◽  
Vol 33 (1) ◽  
pp. 196-199
Author(s):  
Etuji Shiota ◽  
Masao Eguchi ◽  
Hideya Kawamura ◽  
Kenichiro Shibata ◽  
Fumio Wada ◽  
...  
Keyword(s):  
Low Dose ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium ◽  
Experimental Osteoporosis

Faecal calcium excretion does not decrease during long-term feeding of a low-calcium diet in adult dogs

2017 ◽  
Vol 102 (2) ◽  
pp. e798-e805 ◽  
Author(s):  
S. Schmitt ◽  
J. Mack ◽  
E. Kienzle ◽  
L. G. Alexander ◽  
P. J. Morris ◽  
...  
Keyword(s):  
Calcium Excretion ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

Effect of adrenal steroids on bone resorption in rats

1976 ◽  
Vol 230 (1) ◽  
pp. 90-93 ◽  
Author(s):  
S Yasumura
Keyword(s):  
Bone Density ◽  
Bone Resorption ◽  
Serum Calcium ◽  
Adrenal Steroids ◽  
Calcium Diet ◽  
Inhibit Bone Resorption ◽  
Low Calcium Diet ◽  
Low Calcium ◽  
Radiographic Technique ◽  
Parathyroid Extract

Rats labeled with strontium-85 (85Sr) were rejected with adrenocortical steroids for 2 wk. The urinary-to-tibial (U/T) 85Sr ratio was used as an index of bone resorption. The glucocorticoids caused an inhibition of skeletal resorption, as judged by the 50% reduction in the U/T ratio, and decreased excretion of hydroxyproline. Thyroidal calcitonin levels were slightly elevated in glucocorticoid-treated animals, suggestive of a possible retardation of calcitonin release. The U/T ratios of thyroparathyroidectomized (TPTX) rats injected with corticosteroids were 50% of control values. The results indicate that glucocorticoids inhibit bone resorption independent of the action of calcitonin. Cortisol treatment increased the tibial density as measured by a radiographic technique. However, bone density was decreased and the U/T ratio increased in steroid-treated rats fed a low-calcium diet. In TPTX cortisol-treated rats, parathyroid extract (PTE) increased the U/T ratio and serum calcium but not to the degree observed in TPTX PTE-injected control animals. These experiments indicate that in rats glucocorticoids inhibit the rate of bone resorption but this effect can be overcome in part by PTE.

scholarly journals Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Zhengwang Yu ◽  
Jie Huang ◽  
Zhongxin Zhou
Keyword(s):  
Metabolic Diseases ◽  
Chinese Herb ◽  
Therapeutic Effects ◽  
Mineral Density ◽  
Steroid Biosynthesis ◽  
Metabolic Pathway Analysis ◽  
Effective Prevention ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

AbstractCage layer osteoporosis (CLO) is a common bone metabolism disease in the breeding industry of China. However, effective prevention for CLO has not been developed. Icariin (ICA), the main bioactive component of the Chinese herb Epimedium, has been shown to have good therapeutic effects on bone-related diseases. In this study, the effects of ICA were further evaluated in a low-calcium diet-induced CLO, and a serum metabolomics assay was performed to understand the underlying mechanisms. A total of 144 31-wk-old Lohmann pink-shell laying hens were randomly allocated to 4 groups with 6 replicates of 6 hens per replicate. The 4 dietary treatment groups consisted of a basal diet (3.5% calcium), a low-calcium diet (2.0% calcium), and a low-calcium diet supplemented with 0.5 or 2.0 g/kg ICA. The results showed that ICA exerted good osteoprotective effects on low-calcium diet-induced CLO. ICA significantly increased femur bone mineral density, improved bone microstructure, decreased bone metabolic level, and upregulated mRNA expression of bone formation genes in femoral bone tissue. Serum untargeted metabolomics analysis showed that 8 metabolite levels were significantly changed after ICA treatment, including increased contents of 7-dehydrocholesterol, 7-oxocholesterol, desmosterol, PC (18:1(9Z)/18:1(9Z)), PS (18:0/18:1(9Z)), N,N-dimethylaniline and 2-hydroxy-butanoic acid and decreased N2,N2-dimethylguanosine. Metabolic pathway analysis based on the above 8 metabolites indicated that ICA mainly perturbed steroid biosynthesis and glycerophospholipid metabolism. These findings suggest that ICA can effectively prevent bone loss in low-calcium diet-induced CLO by mediating steroid biosynthesis and glycerophospholipid metabolism and provide new information for the regulation of bone metabolic diseases.

Low-calcium diet increases blood pressure and alters peripheral but not central angiotensin II binding sites in rats

1989 ◽  
Vol 7 (5) ◽  
pp. 423-427 ◽  
Author(s):  
Samarendra N. Baksi ◽  
Raymond H. Abhold ◽  
Robert C. Speth
Keyword(s):  
Blood Pressure ◽  
Angiotensin Ii ◽  
Binding Sites ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium
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