scholarly journals Tuning endothelial monolayer adhesion: a neutron reflectivity study

2014 ◽  
Vol 306 (1) ◽  
pp. L1-L9 ◽  
Author(s):  
Luka Pocivavsek ◽  
Ann Junghans ◽  
Noureddine Zebda ◽  
Konstantin Birukov ◽  
Jaroslaw Majewski

Endothelial cells, master gatekeepers of the cardiovascular system, line its inner boundary from the heart to distant capillaries constantly exposed to blood flow. Interendothelial signaling and the monolayers adhesion to the underlying collagen-rich basal lamina are key in physiology and disease. Using neutron scattering, we report the first ever interfacial structure of endothelial monolayers under dynamic flow conditions mimicking the cardiovascular system. Endothelial adhesion (defined as the separation distance ℓ between the basal cell membrane and solid boundary) is explained using developed interfacial potentials and intramembrane segregation of specific adhesion proteins. Our method provides a powerful tool for the biophysical study of cellular layer adhesion strength in living tissues.

2014 ◽  
Vol 28 (30) ◽  
pp. 1430015 ◽  
Author(s):  
Ann Junghans ◽  
Mary Jo Waltman ◽  
Hillary L. Smith ◽  
Luka Pocivavsek ◽  
Noureddine Zebda ◽  
...  

Neutron reflectometry (NR) was used to examine various live cells' adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutron reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell — surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment, e.g. for the development of targeted anti-invasive therapies.


Soft Matter ◽  
2018 ◽  
Vol 14 (16) ◽  
pp. 3135-3150 ◽  
Author(s):  
Yussif Saaka ◽  
Daniel T. Allen ◽  
Yuvared Luangwitchajaroen ◽  
Yanan Shao ◽  
Richard A. Campbell ◽  
...  

Specular neutron reflectivity and molecular dynamic simulations have been used to study the interfacial structure and composition of monolayers of SDS containing a low-dose, poorly soluble drug, testosterone enanthate.


1994 ◽  
Vol 351 ◽  
Author(s):  
G. J. Kellogg ◽  
A.M. Mayes ◽  
W. B. Stockton ◽  
M. Ferreira ◽  
M.F. Rubner ◽  
...  

ABSTRACTThe multilayer organization of sulfonated polyaniline/polyallylamine (SPAN/PAH) films was investigated by neutron reflectivity. Films were prepared by sequential adsorption of polycations and polyanions from dilute solution. Scattering contrast was achieved by selective deuteration of blocks of bilayers at varying intervals along the films. The internal organization of the multilayer structure was found to decay significantly with increasing number of bilayer depositions. In addition, a high degree of roughness was determined at the free surfaces of the films. Together, these results suggest that both conformal and nonconformal roughness are present in the films, i.e., defects introduced during individual layer depositions are to some degree transferred to the surface of subsequently deposited layers.


2017 ◽  
Vol 142 (2) ◽  
pp. 825-837 ◽  
Author(s):  
Magne Aanes ◽  
Remi Andre Kippersund ◽  
Kjetil Daae Lohne ◽  
Kjell-Eivind Frøysa ◽  
Per Lunde

1994 ◽  
Vol 376 ◽  
Author(s):  
Charles F. Majkrzak

ABSTRACTAn introduction to the methodology of neutron reflectometry is. given in which the fundamental aspects regarding the actual performance of specular reflection measurements and subsequent analysis of the data are described. The application of this technique to the determination of interfacial structure or composition in thin film and multilayer materials of interest in the fields of magnetism, superconductivity, polymer science, electrochemistry, and biology is illustrated by specific examples. The microscopic information provided by neutron reflectivity which complements that obtained by other probes is emphasized, in particular information which is obtainable because of the inherent isotopic (most notably in the case of hydrogenous materials) or magnetic moment (both magnitude and orientation) sensitivity of the neutron.


2015 ◽  
Vol 39 (1) ◽  
pp. 115-123
Author(s):  
Wei Hua Ho ◽  
Omar Faruq bin Idris ◽  
Tze How New

Under certain flow conditions, when an inlet is aspirated in close proximity to a solid boundary, a vortex will form between the surface and the inlet. The formation and ingestion of such vortices could potentially lead to inefficient fluid suction by pumps or catastrophic damages in high-speed jet engines. Previous studies established the basic relationship of such inlet vortices formation threshold and geometry and flow conditions, though they were typically considered at significantly high Reynolds numbers. It remains unclear if there is a lower limit to the Reynolds number at which this phenomenon ceases to exist. This study shows that this phenomenon exists even at low Reynolds number of Re = 160. In particular, the results are generally in agreement with the previously established relationships at much higher Reynolds numbers but certain correlations are not as significant. This suggests that formation of inlet vortices may be less sensitive to Reynolds numbers effects than previously thought.


2021 ◽  
Vol 9 (9) ◽  
pp. 983
Author(s):  
Xiaofei Cheng ◽  
Chang Liu ◽  
Qilong Zhang ◽  
Ming He ◽  
Xifeng Gao

By adding a cylindrical airbag on the leeward side of a cuboid pontoon, a new-type double-row floating breakwater is designed to improve the wave attenuation performance, and its hydrodynamic characteristics are studied through numerical simulations. First, based on the smoothed particle hydrodynamics (SPH) method, a numerical model used to simulate the interaction between waves and moored floating bodies is built. The fluid motion is governed by the Navier–Stokes equations. The motion of the floating body is computed according to Newton’s second law. The modified dynamic boundary condition is employed to treat the solid boundary. The lumped-mass method is adopted to implement the mooring system. Then, two physical model experiments on waves interaction with cuboid and dual cylindrical floating pontoons are reproduced. By comparing the experimental and numerical wave transmission coefficients, wave reflection coefficients, response amplitude operators and mooring force, the reliability of the numerical model is validated. Finally, the validated numerical model is applied to study the influence of separation distance and wave parameters on the hydrodynamic characteristics of the double-row floating breakwater. The results indicate that the optimal separation distance between pontoon and airbag is 0.75 times the wavelength. At such separation distance and within the concerned 1–4 m wave heights and 4–7 s wave periods, the pontoon-airbag system presents better wave attenuation performance than a single pontoon. This improvement weakens as wave height increases while it strengthens as the wave period increases. In addition, the double-row floating breakwater is more effective in a high-wave regime than in a low-wave regime. In the case of short waves, attention should be paid to the stability and mooring reliability of the seaward pontoon, while in the case of long waves, care needs to be taken of the leeward airbag.


Langmuir ◽  
2014 ◽  
Vol 30 (20) ◽  
pp. 5880-5887 ◽  
Author(s):  
Benjamin J. Cowsill ◽  
Xiubo Zhao ◽  
Thomas A. Waigh ◽  
Saji Eapen ◽  
Robert Davies ◽  
...  

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