Interleukin-8 and monocyte chemoattractant protein-1 mRNAs in oxygen-injured rabbit lung

1995 ◽  
Vol 268 (5) ◽  
pp. L826-L831 ◽  
Author(s):  
C. T. D'Angio ◽  
R. A. Sinkin ◽  
M. B. LoMonaco ◽  
J. N. Finkelstein
Keyword(s):  
Mrna Expression ◽  
Inflammatory Cells ◽  
Interleukin 8 ◽  
Polymorphonuclear Cells ◽  
Rabbit Lung ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Oxygen Exposure ◽  
Pulmonary Response

The chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) are neutrophil and monocyte attractants, respectively. We hypothesized that IL-8 and MCP-1 mRNA expression in alveolar macrophages (AM) lavaged from rabbit lung would be increased by oxygen exposure, which is known to induce inflammation. Adult rabbits were exposed to > 95% oxygen for up to 64 h and allowed to recover in room air for up to 72 h before killing and pulmonary lavage. Numbers of lavageable polymorphonuclear cells (PMN) and AM rose during the exposure protocol. Quantitative in situ hybridization with 3H-labeled cRNA probes showed both IL-8 and MCP-1 mRNA expression in AM during oxygen exposure, with peak levels of IL-8 mRNA at 56-h oxygen exposure and of MCP-1 mRNA at 64-h oxygen exposure with 24-h room air recovery. IL-8 mRNA was present in PMN between 48-h oxygen exposure and 64-h oxygen exposure with 24-h room air recovery. MCP-1 mRNA was not expressed in PMN. This pattern of chemokine mRNA expression emphasizes the importance of inflammatory cells as effectors in the pulmonary response to oxygen exposure.

scholarly journals Induction of Cell-Associated Chemokines after Endotoxin Administration to Healthy Humans

2001 ◽  
Vol 69 (4) ◽  
pp. 2736-2738 ◽  
Author(s):  
Dariusz P. Olszyna ◽  
Evert De Jonge ◽  
Pascale E. P. Dekkers ◽  
Sander J. H. van Deventer ◽  
Tom van der Poll
Keyword(s):  
Plasma Concentrations ◽  
Antigen Receptor ◽  
Interleukin 8 ◽  
Polymorphonuclear Cells ◽  
Monocyte Chemoattractant Protein 1 ◽  
Endotoxin Administration ◽  
Monocyte Chemoattractant ◽  
Duffy Antigen ◽  
Monocyte Chemoattractant Protein ◽  
Healthy Humans

ABSTRACT Erythrocytes express the Duffy antigen receptor for chemokines. Endotoxin injection into humans induced high levels of interleukin-8 (IL-8), growth-related oncogene α, and monocyte chemoattractant protein 1 in circulating erythrocytes. IL-8 was also recovered from mononuclear and polymorphonuclear cells. Cell-associated chemokines may more accurately reflect their production than plasma concentrations.

scholarly journals Production of chemokines, interleukin-8 and monocyte chemoattractant protein-1, during monocyte: endothelial cell interactions

Blood ◽  
1995 ◽  
Vol 86 (7) ◽  
pp. 2767-2773 ◽  
Author(s):  
NW Lukacs ◽  
RM Strieter ◽  
V Elner ◽  
HL Evanoff ◽  
MD Burdick ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Interactions ◽  
Interleukin 8 ◽  
Mrna Levels ◽  
Ifn Gamma ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Soluble Collagen

The extravasation of leukocytes from the lumen of the vessel to a site of inflammation requires specific binding events. The interaction of leukocytes with endothelium, via specific receptors, may provide intracellular signals that activate extravasating cells. In the present study, we have investigated the production of chemokines, interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1) during monocyte: endothelial cell interactions. Both unstimulated and interferon-gamma (IFN-gamma)-prestimulated human umbilical vein endothelial cells (HUVEC) produced low constitutive levels of IL-8 and MCP-1. The addition of enriched monocytes with unstimulated HUVEC resulted in synergistic increases in production of both IL-8 and MCP-1. Monocytes cultured with IFN-gamma-preactivated HUVECs demonstrated little additional increase in IL-8 and MCP-1 production in coculture assays compared with unstimulated HUVEC. Northern blot analysis paralleled the protein data, demonstrating upregulated expression of IL-8 and MCP-1 mRNA in stimulated and unstimulated coculture assays. Culture of enriched monocytes and endothelial cells in transwells demonstrated no increases in IL-8 or MCP-1, indicating the necessity for cellular contact for chemokine production. In previous investigations, we have demonstrated that increased monocyte-derived MIP-1 alpha production was induced by intracellular adhesion molecule-1 (ICAM-1) interactions on activated HUVECs. In contrast, addition of anti-ICAM-1 monoclonal antibodies (MoAbs) did not diminish the production of IL-8 and MCP-1 in the present study. Furthermore, neither antibodies to IL-1 nor tumor necrosis factor (TNF) diminished the production of either IL-8 or MCP- 1. However, when soluble matrix proteins were added to the coculture to block cellular interactions, the chemokine protein and mRNA levels were significantly decreased. IL-8 production was decreased by both soluble collagen and fibronectin, whereas MCP-1 was decreased by only soluble collagen, suggesting differential activation pathways. These results indicate that IL-8 and MCP-1 production are increased during monocyte and endothelial cell interactions in part due to matrix protein binding mechanisms. This mechanism may serve a role in cell activation, production of chemokines, as well as extravasation and recruitment of additional leukocytes during inflammatory responses.

Increased Endothelial Monocyte Chemoattractant Protein-1 and Interleukin-8 in Preeclampsia

2002 ◽  
Vol 100 (4) ◽  
pp. 706-714 ◽  
Author(s):  
Scott Kauma ◽  
Peter Takacs ◽  
Constantine Scordalakes ◽  
Scott Walsh ◽  
Kermic Green ◽  
...  
Keyword(s):  
Interleukin 8 ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein

Spontaneous Production of Monocyte Chemoattractant Protein-1 and Interleukin-8 by the Human Lumbar Intervertebral Disc

Spine ◽  
2002 ◽  
Vol 27 (13) ◽  
pp. 1402-1407 ◽  
Author(s):  
John G. Burke ◽  
R. William G. Watson ◽  
Damien McCormack ◽  
Frank E. Dowling ◽  
Martin G. Walsh ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Interleukin 8 ◽  
Lumbar Intervertebral Disc ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Spontaneous Production

scholarly journals Reduction of Monocyte Chemoattractant Protein-1 and Interleukin-8 Levels by Ticlopidine in TNF-αStimulated Human Umbilical Vein Endothelial Cells

2009 ◽  
Vol 2009 ◽  
pp. 1-10 ◽  
Author(s):  
Chaur-Jong Hu ◽  
Yueh-Lun Lee ◽  
Neng-Yao Shih ◽  
Yi-Yuan Yang ◽  
Suparat Charoenfuprasert ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Thrombus Formation ◽  
Umbilical Vein ◽  
Interleukin 8 ◽  
Monocyte Chemoattractant Protein 1 ◽  
Human Umbilical Vein ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Treatment And Prevention ◽  
Umbilical Vein Endothelial Cells

Atherosclerosis and its associated complications represent major causes of morbidity and mortality in the industrialized or Western countries. Monocyte chemoattractant protein-1 (MCP-1) is critical for the initiating and developing of atherosclerotic lesions. Interleukin-8 (IL-8), a CXC chemokine, stimulates neutrophil chemotaxis. Ticlopidine is one of the antiplatelet drugs used to prevent thrombus formation relevant to the pathophysiology of atherothrombosis. In this study, we found that ticlopidine dose-dependently decreased the mRNA and protein levels of TNF-α-stimulated MCP-1, IL-8, and vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs). Ticlopidine declined U937 cells adhesion and chemotaxis as compared to TNF-αstimulated alone. Furthermore, the inhibitory effects were neither due to decreased HUVEC viability, nor through NF-kB inhibition. These results suggest that ticlopidine decreased TNF-αinduced MCP-1, IL-8, and VCAM-1 levels in HUVECs, and monocyte adhesion. Therefore, the data provide additional therapeutic machinery of ticlopidine in treatment and prevention of atherosclerosis.

An Arthrogenic Alphavirus Induces Monocyte Chemoattractant Protein-1 and Interleukin-8

Intervirology ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Luis Mateo ◽  
May La Linn ◽  
Sean R. McColl ◽  
Simone Cross ◽  
Joy Gardner ◽  
...  
Keyword(s):  
Interleukin 8 ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein
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