Relationship between body mass, tissue metabolic rate, and sodium pump activity in mammalian liver and kidney

1995 ◽  
Vol 268 (3) ◽  
pp. R641-R650 ◽  
Author(s):  
P. Couture ◽  
A. J. Hulbert
Keyword(s):  
Metabolic Rate ◽  
Body Mass ◽  
Sodium Pump ◽  
Mammalian Species ◽  
Kidney Cortex ◽  
Tissue Metabolism ◽  
Pump Activity ◽  
Liver And Kidney ◽  
Cortex Slices ◽  
Kidney Cortex Slices

The allometric relationship between body mass and tissue metabolism was examined in liver and kidney cortex slices from mouse, rat, rabbit, sheep, and cattle, representing an approximately 12,000-fold difference in body mass and an 11-fold difference in mass-specific basal metabolic rate. Larger mammals have lower tissue metabolic rates (TMR; mumol O2.g wet wt-1.min-1) at 37 degrees C, yielding the equations TMR = 3.6 M-0.21 for liver slices and TMR = 3.2 M-0.11 for kidney cortex slices, where M is body mass in grams. This appears to be an intrinsic property of the tissue and is not due to differences in extracellular space or tissue protein content, because these are relatively constant in all mammalian species examined. The allometric relationships remain when tissue metabolism is expressed relative to "active cell mass" in tissue slices. Potassium uptake rate (KUR; mumol K+.g wet wt-1.min-1) was also measured (as 86Rb+ uptake) and was also lower in larger mammals, yielding the equations KUR = 1.2 M-0.14 in liver slices and KUR = 3.4 M-0.13 for kidney cortex slices. The energetic costs of sodium pump activity were estimated to be < 10% of TMR for liver and kidney cortex from all five mammalian species.

scholarly journals The Effect of Temperature on the Uptake of Radiosulfate by Rat Renal Tissue from Radiosulfate-Containing Solutions in Vitro

1961 ◽  
Vol 44 (3) ◽  
pp. 555-569 ◽  
Author(s):  
Ingrith J. Deyrup ◽  
R. E. Davies
Keyword(s):  
Rat Liver ◽  
Incubation Medium ◽  
Renal Tissue ◽  
Effect Of Temperature ◽  
Kidney Cortex ◽  
Liver And Kidney ◽  
Cortex Slices ◽  
Kidney Cortex Slices

Kidney cortex slices incubated in vitro at 0°C. accumulate radiosulfate from the incubation medium. This process differs from the previously described uptake of radiosulfate by renal tissue incubated at 38°C., for instance, in the lesser sensitivity of the uptake at 0°C. to differential effects of Na+ as compared with K+ ions, and of sucrose as compared with glucose. Phlorizin inhibits radiosulfate accumulation at 0°C., whereas it enhances the uptake at 38°C. Effects of the cations K+ and Na+ and of phlorizin at temperatures intermediate between 0° and 38°C. have been studied. Parallels have been noted between the accumulative processes for radiosulfate of kidney slices maintained at 0°C. and of mitochondria isolated from rat liver and kidney cortex. These similarities may be attributed to an important role of radiosulfate uptake by mitochondria in slice accumulation of radiosulfate in the cold.

Distribution of water between intracellular and extracellular phases in incubated tissue slices

1960 ◽  
Vol 199 (6) ◽  
pp. 1223-1226 ◽  
Author(s):  
A. G. Swan ◽  
E. E. Ellington ◽  
A. T. Miller
Keyword(s):  
Kidney Cortex ◽  
Tissue Water ◽  
Tissue Slices ◽  
Pig Liver ◽  
Liver Slices ◽  
Inulin Space ◽  
Liver And Kidney ◽  
Cortex Slices ◽  
Kidney Cortex Slices ◽  
Guinea Pig Liver

Guinea pig liver and kidney cortex slices swelled when incubated under a variety of abnormal metabolic and osmotic conditions. Simultaneous measurement of the inulin space permitted the calculation of the distribution of the water of swelling between the intracellular and extracellular compartments. Swelling was entirely intracellular in the kidney cortex slices, while both intracellular and extracellular compartments were involved in the swelling of liver slices. Under all the conditions studied, kidney slices had a greater capacity for resisting changes in the volume and distribution of tissue water than did the liver slices.

scholarly journals The effects of dietary conditions and glycerol concentration on glycerol uptake by rat liver and kidney-cortex slices

1969 ◽  
Vol 112 (4) ◽  
pp. 449-453 ◽  
Author(s):  
Janice Robinson ◽  
E A Newsholme
Keyword(s):  
Kidney Cortex ◽  
Glycerol Concentration ◽  
Carbohydrate Diet ◽  
Low Carbohydrate Diet ◽  
Low Carbohydrate ◽  
Glycerol Utilization ◽  
Liver And Kidney ◽  
Cortex Slices ◽  
Glycerol Uptake ◽  
Kidney Cortex Slices

1. Glycerol utilization by rat liver and kidney-cortex slices was studied in an attempt to define factors that might be important in the regulation of glycerol utilization by these tissues in vivo; the formation of glucose from glycerol by kidney-cortex slices was also studied. 2. The rate of glycerol uptake by liver slices was not changed (in comparison with the normal fed control) by starvation (48hr.), feeding with a low-carbohydrate diet (4–8 days) or feeding with a diet containing 25% glycerol (up to 18 days). Similarly, starvation or a low-carbohydrate diet had no effect on uptake by kidney-cortex slices; however, feeding with the glycerol diet increased glycerol uptake by kidney-cortex slices. 3. The rates of glycerol uptake by slices from both tissues were increased on raising the glycerol concentration from 0·2mm to 2·5 or 5·0mm. 4. Starvation increased the conversion of glycerol into glucose by kidney-cortex slices, but there was no effect of the low-carbohydrate diet; the rate of glucose formation was increased by feeding with the 25%-glycerol diet and was proportional to the increase in glycerol uptake. The rate of glucose production by these slices was increased by raising the glycerol concentration in the incubation medium from 0·2mm to 1·0mm, but, except for the slices from animals receiving the 25%-glycerol diet, there was no effect above 1·0mm-glycerol. 5. The significance of plasma glycerol concentration in regulating glycerol uptake by these tissues is discussed.

Studies on glucose synthesis by rat liver and kidney cortex slices

Life Sciences ◽  
1966 ◽  
Vol 5 (7) ◽  
pp. 655-663 ◽  
Author(s):  
S.R. Wagle ◽  
R.K. Gaskins ◽  
Annette Jacoby ◽  
J. Ashmore
Keyword(s):  
Rat Liver ◽  
Kidney Cortex ◽  
Liver And Kidney ◽  
Glucose Synthesis ◽  
Cortex Slices ◽  
Kidney Cortex Slices
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