The functional regeneration of syncytiotrophoblast in cultured explants of term placenta

2001 ◽  
Vol 280 (4) ◽  
pp. R1116-R1122 ◽  
Author(s):  
C. M. Simán ◽  
C. P. Sibley ◽  
C. J. P. Jones ◽  
M. A. Turner ◽  
S. L. Greenwood
Keyword(s):  
Lactate Dehydrogenase ◽  
Culture System ◽  
Culture Medium ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Ang Ii ◽  
Placental Function ◽  
Culture Time ◽  
Functional Regeneration

We have investigated the functional characteristics of term human placental villous explants kept in long-term (7–11 days) culture. Fragments of placental villous tissue (∼5–10 mg wet wt) were cultured in supplemented CMRL-1066 culture medium for up to 11 days. After the first day of culture, the syncytiotrophoblast appeared vacuolated and eventually degenerated. However, a new syncytiotrophoblast developed by day 4, being indistinguishable from that of a fresh placenta by 11 days. Release of human chorionic gonadotrophin increased and activity of lactate dehydrogenase in culture medium decreased with culture time. Transport variables were measured over the first 7 days of culture. Basal86Rb efflux was reduced with time in culture and was inhibited by Ba2+, suggesting the efflux was mediated by K+ channels. At all stages of culture, 86Rb efflux was stimulated by ATP, hyposmotic medium, and ANG II. A complex pattern of efflux changes with culture time and type of stimulator was observed, suggesting that several compartments of the tissue contributed to stimulated efflux. This culture system provides opportunities for studies of chronic regulation of placental function.

INFLUENCE OF PROSTAGLANDINS AND HUMAN CHORIONIC GONADOTROPHIN ON PROGESTERONE CONCENTRATION AND OOCYTE MATURATION IN MOUSE OVARIAN FOLLICLES MAINTAINED IN ORGAN CULTURE

1975 ◽  
Vol 65 (1) ◽  
pp. 19-25 ◽  
Author(s):  
P. NEAL ◽  
T. G. BAKER ◽  
K. P. McNATTY ◽  
R. J. SCARAMUZZI
Keyword(s):  
Organ Culture ◽  
Culture Medium ◽  
Control Level ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Prostaglandin E ◽  
Progesterone Concentration ◽  
Histological Changes ◽  
Control Value ◽  
Prostaglandin F

SUMMARY The response of mouse ovaries maintained in organ culture to prostaglandin E2 (PGE2) and prostaglandin F2α (F2α) was assessed using quantitative histological and radioimmunoassay procedures. Prostaglandin E2 induced histological changes in the cultured follicles comparable to those induced by human chorionic gonadotrophin (HCG) and the increase in the number of oocytes undergoing preovulatory maturation over the control value was the same irrespective of the treatment (PGE2 alone, HCG alone, or PGE2 + HCG). The amount of progesterone/ ml of culture medium was also significantly higher with these preparations than in control cultures (about 125 ng/ml compared with 57 ng/ml). By contrast, 5 μg PGF2α/ml medium increased neither the number of oocytes undergoing maturation nor the concentration of progesterone in the culture medium. The latter increased when the dose of PGF2α was increased to 30 μg/ml, although the proportion of oocytes beyond the dictyate stage remained at the control level. There was no augmentation in the response (above the level for HCG alone) when HCG and PGF2α were added to the explant medium simultaneously. These results are discussed in terms of the possible mechanism of action of the various preparations.

Seed production technology and culture system of snakeskin gouramy (Trichogaster pectoralis) in Vietnam.

2020 ◽  
Author(s):  
Nhat Lung Dung
Keyword(s):  
Survival Rate ◽  
Pituitary Gland ◽  
Culture System ◽  
Mekong Delta ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Artificial Reproduction ◽  
Technology And Culture ◽  
Natural Water Bodies ◽  
Different Doses

Abstract Snakeskin gouramy (Trichogaster pectoralis) is a high-value fish species in the Mekong Delta of Vietnam. It is naturally distributed in Bac Lieu, Ca Mau and Kien Giang provinces but production from natural water bodies has declined significantly. Snakeskin gouramy can be cultured in monoculture or polyculture, in ponds or rice fields. Artificial reproduction of the fish was studied using different doses of human chorionic gonadotrophin (HCG) and pituitary gland. At a dose of 1,000-1,500 UI of HGC and 0.54 mg of pituitary gland, fish spawned after 18-19 h at a water temperature of 27-28°C. Fish can re-mature 3-4 times per year. Fry was nursed at a density of 300-900 fry m-2 with survival rate of 75-78%.

Artificial Tissues in Perfusion Culture

1997 ◽  
Vol 20 (1) ◽  
pp. 57-62 ◽  
Author(s):  
M. Sittinger ◽  
O. Schultz ◽  
G. Keyszer ◽  
W.W. Minuth ◽  
G.R. Burmester
Keyword(s):  
Culture System ◽  
Culture Medium ◽  
Three Dimensional ◽  
Perfusion Culture ◽  
Traditional Culture ◽  
Polymer Fibers ◽  
Support Material ◽  
Perfusion System ◽  
Long Term Experiments

In the stagnant environment of traditional culture dishes it is difficult to generate long term experiments or artificial tissues from human cells. For this reason a perfusion culture system with a stable supply of nutrients was developed. Human chondrocytes were seeded three-dimensionally in resorbable polymer fleeces. The cell-polymer tissues were then mounted in newly developed containers (W. W. Minuth et al, Biotechniques, 1996) and continuously perfused by fresh medium for 40 days. Samples from the effluate were analyzed daily, and the pH of the medium and glucose concentration remained stable during this period. The lactid acid concentration increased from 0,17 mg/ml to 0,35 mg/ml, which was influenced by the degradation of the resorbable polymer fibers used as three dimensional support material for the cells. This perfusion system proved to be reliable especially in long term cultures. Any components in the culture medium of the cells could be monitored without disturbances as caused by manual medium replacement. These results suggest the described perfusion culture system to be a valuable and convenient tool for many applications in tissue engineering, especially in the generation of artificial connective tissue.

Long-term outcome of patients with persistent low-level elevation of human chorionic gonadotrophin

2016 ◽  
Vol 42 (6) ◽  
pp. 694-700 ◽  
Author(s):  
Xue-qian Qian ◽  
Li-li Chen ◽  
Bao-hua Li ◽  
Xiao-dong Cheng ◽  
Xiao-yun Wan
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Term Outcome ◽  
Long Term Outcome ◽  
Low Level ◽  
Level Elevation
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