Identification of functional lung unit in the dog by graded vascular embolization

1980 ◽  
Vol 49 (1) ◽  
pp. 132-141 ◽  
Author(s):  
I. Young ◽  
R. W. Mazzone ◽  
P. D. Wagner
Keyword(s):  
Gas Exchange ◽  
Histological Examination ◽  
Inert Gas ◽  
Polystyrene Beads ◽  
Lung Unit ◽  
Bead Size ◽  
Exchange Unit ◽  
Ventilation Perfusion Ratio

To study the dimensions of the functional gas exchange unit, spherical polystyrene beads (diam 50-500 micrometers) were injected intravenously into 12 normal anesthetized paralyzed dogs (15-24 kg wt). We argued that beads small enough to lodge within gas exchange units would not give rise to a population of high ventilation-perfusion ratio (VA/Q) areas, whereas embolization of larger vessels supplying these units would. Each dog received only one bead size in cumulative 0.25-g doses up to a maximum of 2.25 g. Multiple inert gas elimination data were obtained after each dose to monitor the development of high VA/Q regions. Injection of 50- and 100-micrometers beads never gave rise to high VA/Q regions, whereas 150-, 250-, and 500-micrometers beads consistently induced a high VA/Q mode comprising up to 45% of the ventilation. Histological examination of lungs from five additional dogs injected with small (approximately 0.5 g) doses revealed that beads rarely formed clusters and appeared in vessels of their own diameter in over 90% of instances. By the above criterion, the functional gas exchange unit in these lungs is that volume of tissue subtended by 150- micrometers-diam arteries (vessels associated with respiratory bronchioles).

Effects of Vaporized Perfluorocarbon on Pulmonary Blood Flow and Ventilation/Perfusion Distribution in a Model of Acute Respiratory Distress Syndrome

2001 ◽  
Vol 95 (6) ◽  
pp. 1414-1421 ◽  
Author(s):  
Matthias Hübler ◽  
Jennifer E. Souders ◽  
Erin D. Shade ◽  
Nayak L. Polissar ◽  
Carmel Schimmel ◽  
...  
Keyword(s):  
Blood Flow ◽  
Oleic Acid ◽  
Gas Exchange ◽  
Lung Injury ◽  
Repeated Measures ◽  
Pulmonary Blood Flow ◽  
Analysis Of Covariance ◽  
Inert Gas ◽  
Low Flow ◽  
Repeated Measures Analysis

Background Perfluorocarbon (PFC) liquids are known to improve gas exchange and pulmonary function in various models of acute respiratory failure. Vaporization has been recently reported as a new method of delivering PFC to the lung. Our aim was to study the effect of PFC vapor on the ventilation/perfusion (VA/Q) matching and relative pulmonary blood flow (Qrel) distribution. Methods In nine sheep, lung injury was induced using oleic acid. Four sheep were treated with vaporized perfluorohexane (PFX) for 30 min, whereas the remaining sheep served as control animals. Vaporization was achieved using a modified isoflurane vaporizer. The animals were studied for 90 min after vaporization. VA/Q distributions were estimated using the multiple inert gas elimination technique. Change in Qrel distribution was assessed using fluorescent-labeled microspheres. Results Treatment with PFX vapor improved oxygenation significantly and led to significantly lower shunt values (P < 0.05, repeated-measures analysis of covariance). Analysis of the multiple inert gas elimination technique data showed that animals treated with PFX vapor demonstrated a higher VA/Q heterogeneity than the control animals (P < 0.05, repeated-measures analysis of covariance). Microsphere data showed a redistribution of Qrel attributable to oleic acid injury. Qrel shifted from areas that were initially high-flow to areas that were initially low-flow, with no difference in redistribution between the groups. After established injury, Qrel was redistributed to the nondependent lung areas in control animals, whereas Qrel distribution did not change in treatment animals. Conclusion In oleic acid lung injury, treatment with PFX vapor improves gas exchange by increasing VA/Q heterogeneity in the whole lung without a significant change in gravitational gradient.

Effects of acetone in heparin on the multiple inert gas elimination technique

1985 ◽  
Vol 58 (4) ◽  
pp. 1143-1147 ◽  
Author(s):  
F. L. Powell ◽  
F. A. Lopez ◽  
P. D. Wagner
Keyword(s):  
Partial Pressure ◽  
Dead Space ◽  
Room Temperature ◽  
Inert Gas ◽  
Published Data ◽  
Physiological Mechanisms ◽  
Heparinized Saline ◽  
Partial Pressures ◽  
Ventilation Perfusion Ratio ◽  
Mixed Venous

We have detected acetone in several brands of heparin. If uncorrected, this leads to errors in measuring acetone in blood collected in heparinized syringes, as in the multiple inert gas elimination technique for measuring ventilation-perfusion ratio (VA/Q) distributions. Error for acetone retention [R = arterial partial pressure-to-mixed venous partial pressure (P-V) ratio] is usually small, because R is normally near 1.0, and the error is similar in arterial and mixed venous samples. However, acetone excretion [E = mixed expired partial pressure (P-E)-to-P-V ratio] will appear erroneously low, because P-E is accurately measured in dry syringes, but P-V is overestimated. A physical model of a homogeneous alveolar lung at room temperature and without dead space shows: the magnitude of acetone E error depends upon the ratio of blood sample to heparinized saline volumes and acetone partial pressures, without correction, acetone E can be less than that of less soluble gases like ether, a situation incompatible with conventional gas exchange theory, and acetone R and E can be correctly calculated using the principle of mass balance if the acetone partial pressure in heparinized saline is known. Published data from multiple inert gas elimination experiments with acetone-free heparin, in our labs and others, are within the limits of experimental error. Thus the hypothesis that acetone E is anomalously low because of physiological mechanisms involving dead space tissue capacitance for acetone remains to be tested.

Pulmonary gas exchange in humans during exercise at sea level

1986 ◽  
Vol 60 (5) ◽  
pp. 1590-1598 ◽  
Author(s):  
M. D. Hammond ◽  
G. E. Gale ◽  
K. S. Kapitan ◽  
A. Ries ◽  
P. D. Wagner
Keyword(s):  
Gas Exchange ◽  
Sea Level ◽  
Minute Ventilation ◽  
Normal Subjects ◽  
Inert Gas ◽  
Diffusion Limitation ◽  
O2 Uptake ◽  
Heavy Exercise ◽  
Blood Temperature ◽  
O2 Diffusion

Previous studies have shown both worsening ventilation-perfusion (VA/Q) relationships and the development of diffusion limitation during exercise at simulated altitude and suggested that similar changes could occur even at sea level. We used the multiple-inert gas-elimination technique to further study gas exchange during exercise in healthy subjects at sea level. Mixed expired and arterial respiratory and inert gas tensions, cardiac output, heart rate, minute ventilation, respiratory rate, and blood temperature were recorded at rest and during steady-state exercise in the following order: rest, minimal exercise (75 W), heavy exercise (300 W), heavy exercise breathing 100% O2, repeat rest, moderate exercise (225 W), and light exercise (150 W). Alveolar-to-arterial O2 tension difference increased linearly with O2 uptake (VO2) (6.1 Torr X min-1 X 1(-1) VO2). This could be fully explained by measured VA/Q inequality at mean VO2 less than 2.5 l X min-1. At higher VO2, the increase in alveolar-to-arterial O2 tension difference could not be explained by VA/Q inequality alone, suggesting the development of diffusion limitation. VA/Q inequality increased significantly during exercise (mean log SD of perfusion increased from 0.28 +/- 0.13 at rest to 0.58 +/- 0.30 at VO2 = 4.0 l X min-1, P less than 0.01). This increase was not reversed by 100% O2 breathing and appeared to persist at least transiently following exercise. These results confirm and extend the earlier suggestions (8, 21) of increasing VA/Q inequality and O2 diffusion limitation during heavy exercise at sea level in normal subjects and demonstrate that these changes are independent of the order of performance of exercise.

CALIBRATION OF INERT GAS EXCHANGE IN THE MOUSE

1971 ◽  
pp. 179-191 ◽  
Author(s):  
Edward T. Flynn ◽  
C.J. Lambertsen
Keyword(s):  
Gas Exchange ◽  
Inert Gas

Validation of measurements of ventilation-to-perfusion ratio inequality in the lung from expired gas

2003 ◽  
Vol 94 (3) ◽  
pp. 1186-1192 ◽  
Author(s):  
G. Kim Prisk ◽  
Harold J. B. Guy ◽  
John B. West ◽  
James W. Reed
Keyword(s):  
Gas Exchange ◽  
Respiratory Exchange Ratio ◽  
Blood Gases ◽  
Inert Gas ◽  
Phase Iii ◽  
Strongly Correlated ◽  
Arterial Blood Gases ◽  
Arterial Blood ◽  
Expired Gas ◽  
The Relationship

The analysis of the gas in a single expirate has long been used to estimate the degree of ventilation-perfusion (V˙a/Q˙) inequality in the lung. To further validate this estimate, we examined three measures ofV˙a/Q˙ inhomogeneity calculated from a single full exhalation in nine anesthetized mongrel dogs under control conditions and after exposure to aerosolized methacholine. These measurements were then compared with arterial blood gases and with measurements of V˙a/Q˙ inhomogeneity obtained using the multiple inert gas elimination technique. The slope of the instantaneous respiratory exchange ratio (R slope) vs. expired volume was poorly correlated with independent measures, probably because of the curvilinear nature of the relationship due to continuing gas exchange. When R was converted to the intrabreathV˙a/Q˙ (iV˙/Q˙), the best index was the slope of iV˙/Q˙ vs. volume over phase III (iV˙/Q˙slope). This was strongly correlated with independent measures, especially those relating to inhomogeneity of perfusion. The correlations for iV˙/Q˙ slope and R slope considerably improved when only the first half of phase III was considered. We conclude that a useful noninvasive measurement ofV˙a/Q˙ inhomogeneity can be derived from the intrabreath respiratory exchange ratio.

Middle ear gas exchange in isobaric counterdiffusion

1979 ◽  
Vol 47 (6) ◽  
pp. 1239-1244 ◽  
Author(s):  
C. W. Dueker ◽  
C. J. Lambertsen ◽  
J. J. Rosowski ◽  
J. C. Saunders
Keyword(s):  
Nitrous Oxide ◽  
Gas Exchange ◽  
Tympanic Membrane ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Inert Gas ◽  
Pressure Measurements ◽  
Gas Sampling ◽  
Gas Space ◽  
Counter Diffusion

Nitrous oxide entry into the middle ear gas space was studied in cats in relation to anesthesia and the vestibular dysfunction caused by isobaric inert gas counter-diffusion in diving. A catheter implanted in the auditory bulla was used for direct gas sampling and pressure measurements. Experiments were designed to evaluate the participation of the eustachian tube, mucosal blood vessels, and tympanic membrane in middle ear gas exchange. The eustachian tube did not contribute to N2O entry and the mucosal blood supply only contributed about one-third of the total N2O accumulation. Diffusion across the tympanic membrane accounted for most of the N2O entering the middle ear from ambient and respiratory environments containing N2O.

Respiratory and inert gas exchange during high-frequency ventilation

1982 ◽  
Vol 52 (3) ◽  
pp. 683-689 ◽  
Author(s):  
H. T. Robertson ◽  
R. L. Coffey ◽  
T. A. Standaert ◽  
W. E. Truog
Keyword(s):  
Gas Exchange ◽  
High Frequency ◽  
Gas Flow ◽  
Inert Gases ◽  
Inert Gas ◽  
High Frequency Ventilation ◽  
Physiological Dead Space ◽  
Volume Ventilation ◽  
Frequency Ventilation ◽  
Carrier Gases

Pulmonary gas exchange during high-frequency low-tidal volume ventilation (HFV) (10 Hz, 4.8 ml/kg) was compared with conventional ventilation (CV) and an identical inspired fresh gas flow in pentobarbital-anesthetized dogs. Comparing respiratory and infused inert gas exchange (Wagner et al., J. Appl. Physiol. 36: 585--599, 1974) during HFV and CV, the efficiency of oxygenation was not different, but the Bohr physiological dead space ratio was greater on HFV (61.5 +/- 2.2% vs. 50.6 +/- 1.4%). However, the elimination of the most soluble inert gas (acetone) was markedly enhanced by HFV. The increased elimination of the soluble infused inert gases during HFV compared with CV may be related to the extensive intraregional gas mixing that allows the conducting airways to serve as a capacitance for the soluble inert gases. Comparing as exchange during HFV with three different density carrier gases (He, N2, and Ar), the efficiency of elimination of Co2 or the intravenously infused inert gases was greatest with He-O2. However, the alveolar-arterial partial pressure difference for O2 on He-O2 exceeded that on N2-O2 by 5.4 Torr during HFV. The finding agrees with similar observations during CV, suggesting that this aspect of gas exchange is not substantially altered by HFV.

Effects of PEEP and tolazoline infusion on respiratory and inert gas exchange in experimental meconium aspiration

1982 ◽  
Vol 100 (2) ◽  
pp. 284-290 ◽  
Author(s):  
William E. Truog ◽  
Raymond K. Lyrene ◽  
Thomas A. Standaert ◽  
Janet Murphy ◽  
David E. Woodrum
Keyword(s):  
Gas Exchange ◽  
Inert Gas ◽  
Meconium Aspiration
Sign in / Sign up

Export Citation Format

Share Document