Effect of bupropion on hippocampal neurotransmitters and on peripheral hormonal concentrations in the rat

2003 ◽  
Vol 95 (2) ◽  
pp. 652-656 ◽  
Author(s):  
M. F. Piacentini ◽  
R. Clinckers ◽  
R. Meeusen ◽  
S. Sarre ◽  
G. Ebinger ◽  
...  
Keyword(s):  
Significant Variation ◽  
Dopaminergic System ◽  
In Vivo Microdialysis ◽  
Sampling Time ◽  
Microdialysis Probe ◽  
Blood Samples ◽  
Before And After ◽  
Freely Moving ◽  
Vena Femoralis

The purpose of the present study was to administer an acute dose of the dual dopamine norepinephrine reuptake blocker bupropion in freely moving rats and to monitor the extracellular neurotransmitter concentrations in the hippocampus via in vivo microdialysis and the peripheral hormonal concentrations via catheterization. A microdialysis probe was inserted in the hippocampus, and samples for serotonin, dopamine, and norepinephrine were collected every 20 min before and after the injection of 17 mg/kg of bupropion, for a total sampling time of 180 min. A catheter was placed in the vena femoralis of the second group of rats, and blood samples were collected before and after bupropion injection for quantification of growth hormone, prolactin, corticosterone, adrenocorticotropin hormone, and β-endorphins. All neurotransmitter levels (dopamine, norepinephrine, and serotonin) significantly increased after bupropion injection. This was accompanied by a significant decrease in prolactin concentrations, whereas the other hormones showed no statistically significant variation. It can, therefore, be concluded that, although bupropion has dual reuptake proprieties, the observed effects both at the central and at the peripheral level seem to be ruled by the dopaminergic system.

Acute dopamine/norepinephrine reuptake inhibition increases brain and core temperature in rats

2005 ◽  
Vol 99 (4) ◽  
pp. 1397-1401 ◽  
Author(s):  
Hiroshi Hasegawa ◽  
Romain Meeusen ◽  
Sophie Sarre ◽  
Marc Diltoer ◽  
Maria Francesca Piacentini ◽  
...  
Keyword(s):  
Heat Loss ◽  
Preoptic Area ◽  
Sampling Time ◽  
Microdialysis Probe ◽  
Freely Moving Rats ◽  
Before And After ◽  
Body Core ◽  
Freely Moving ◽  
Baseline Levels ◽  
Norepinephrine Reuptake

The purpose of the present study was to examine the effects of an acute dose of the dual dopamine (DA) and norepinephrine (NE) reuptake inhibitor bupropion (Bup) on brain (Tbrain), body core (Tcore), and tail skin (Ttail) temperature in freely moving rats and to simultaneously monitor the extracellular neurotransmitter concentrations in the preoptic area and anterior hypothalamus (PO/AH). A microdialysis probe was inserted in the PO/AH, and samples for NE, DA, and serotonin (5-HT) were collected every 20 min before and after the injection of 17 mg/kg of Bup, for a total sampling time of 180 min. Tcore was monitored using a biotelemetry system. Tbrain and Ttail, an index of heat loss response, were also measured. Both NE and DA levels in the PO/AH significantly increased after Bup injection compared with the baseline levels, reaching ∼450 and 230%, respectively, 40 min after injection. There was no effect on 5-HT release. The neurotransmitter changes were accompanied by a significant decrease in Ttail and an increase in both Tbrain and Tcore compared with the baseline levels. The present results demonstrate that inhibition of NE and DA reuptake suppresses heat loss mechanisms and elevates Tbrain and Tcore in freely moving rats.

Effects of a primary immune response to T-cell dependent antigen on serotonin metabolism in frontal cortex: in vivo microdialysis study in freely moving Fischer 344 rat

1994 ◽  
Vol 645 (1-2) ◽  
pp. 150-156 ◽  
Author(s):  
Alain M. Gardier ◽  
Sébastien Kachaner ◽  
Elisabeth Khan Shaghaghi ◽  
Christian Blot ◽  
Claude Bohuon ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cell ◽  
In Vivo Microdialysis ◽  
Primary Immune Response ◽  
Serotonin Metabolism ◽  
Fischer 344 ◽  
Fischer 344 Rat ◽  
Freely Moving ◽  
Microdialysis Study

scholarly journals Risperidone and 5-HT2A Receptor Antagonists Attenuate and Reverse Cocaine-Induced Hyperthermia in Rats

2020 ◽  
Vol 23 (12) ◽  
pp. 811-820
Author(s):  
Tsuyoshi Okada ◽  
Katsutoshi Shioda ◽  
Akiko Makiguchi ◽  
Shiro Suda
Keyword(s):  
In Vivo Microdialysis ◽  
D1 Receptor ◽  
D1 Receptors ◽  
Receptor Antagonists ◽  
Induced Hyperthermia ◽  
Definitive Therapy ◽  
Before And After ◽  
Sch 23 390 ◽  
Related Mortality

Abstract Background Cocaine (benzoylmethylecgonine) is one of the most widely used illegal psychostimulant drugs worldwide, and mortality from acute intoxication is increasing. Suppressing hyperthermia is effective in reducing cocaine-related mortality, but a definitive therapy has not yet been found. In this study, we assessed the ability of risperidone to attenuate acute cocaine-induced hyperthermia and delineated the mechanism of its action. Methods Rats were injected i.p. with saline, risperidone, ketanserin, ritanserin, haloperidol, or SCH 23 390 before and after injection of cocaine (30 mg/kg) or with WAY-00 635, SB 206 553, or sulpiride before cocaine injection; thereafter, the rectal temperature was measured every 30 minutes for up to 4 hours. In vivo microdialysis was used to reveal the effect of risperidone on cocaine-induced elevation of dopamine (DA), serotonin (5-HT), and noradrenaline concentrations in the anterior hypothalamus. For post-administration experiments, saline or risperidone (0.5 mg/kg) were injected into rats, and cocaine (30 mg/kg) was injected 15 minutes later. For every 30 minutes thereafter, DA, 5-HT, and noradrenaline levels were measured for up to 240 minutes after cocaine administration. Results Risperidone, 5-HT2A receptor antagonists, and D1 receptor antagonistic drugs prevented and reversed cocaine-induced hyperthermia. In contrast, receptor antagonists for 5-HT1A, 5-HT2B/2C, and D2 did not alter cocaine-induced hyperthermia. Risperidone treatment further attenuated cocaine-induced elevation of DA. Conclusions Our results indicate that risperidone attenuates cocaine-induced hyperthermia primarily by blocking the activities of the 5-HT2A and D1 receptors and may be potentially useful for treating cocaine-induced acute hyperthermia in humans.

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