Single neuron dynamics during experimentally induced anoxic depolarization

2013 ◽  
Vol 110 (7) ◽  
pp. 1469-1475 ◽  
Author(s):  
Bas-Jan Zandt ◽  
Tyler Stigen ◽  
Bennie ten Haken ◽  
Theoden Netoff ◽  
Michel J. A. M. van Putten
Keyword(s):  
Single Neuron ◽  
Cell Model ◽  
Dynamical Behavior ◽  
Pyramidal Cells ◽  
Membrane Voltage ◽  
Energy Depletion ◽  
Single Cell Model ◽  
Anoxic Depolarization ◽  
Voltage Dynamics ◽  
Neuron Dynamics

We studied single neuron dynamics during anoxic depolarizations, which are often observed in cases of neuronal energy depletion. Anoxic and similar depolarizations play an important role in several pathologies, notably stroke, migraine, and epilepsy. One of the effects of energy depletion was experimentally simulated in slices of rat cortex by blocking the sodium-potassium pumps with ouabain. The membrane voltage of pyramidal cells was measured. Five different kinds of dynamical behavior of the membrane voltage were observed during the resulting depolarizations. Using bifurcation analysis of a single cell model, we show that these voltage dynamics all are responses of the same cell, with normally functioning ion channels, to particular courses of the intra- and extracellular concentrations of sodium and potassium.

Bursting patterns and mixed-mode oscillations in reduced Purkinje model

2018 ◽  
Vol 32 (05) ◽  
pp. 1850043 ◽  
Author(s):  
Feibiao Zhan ◽  
Shenquan Liu ◽  
Jing Wang ◽  
Bo Lu
Keyword(s):  
Purkinje Cell ◽  
Mixed Mode ◽  
Cell Model ◽  
Dynamical Behavior ◽  
Characteristic Index ◽  
Mixed Mode Oscillations ◽  
Codimension One ◽  
Bifurcation Phenomenon ◽  
Lyapunov Coefficient ◽  
Potential Link

Bursting discharge is a ubiquitous behavior in neurons, and abundant bursting patterns imply many physiological information. There exists a closely potential link between bifurcation phenomenon and the number of spikes per burst as well as mixed-mode oscillations (MMOs). In this paper, we have mainly explored the dynamical behavior of the reduced Purkinje cell and the existence of MMOs. First, we adopted the codimension-one bifurcation to illustrate the generation mechanism of bursting in the reduced Purkinje cell model via slow–fast dynamics analysis and demonstrate the process of spike-adding. Furthermore, we have computed the first Lyapunov coefficient of Hopf bifurcation to determine whether it is subcritical or supercritical and depicted the diagrams of inter-spike intervals (ISIs) to examine the chaos. Moreover, the bifurcation diagram near the cusp point is obtained by making the codimension-two bifurcation analysis for the fast subsystem. Finally, we have a discussion on mixed-mode oscillations and it is further investigated using the characteristic index that is Devil’s staircase.

Calibration of single-cell model parameters based on membrane resistance improves the accuracy of cardiac tissue simulations

2021 ◽  
pp. 101375
Author(s):  
Elnaz Pouranbarani ◽  
Lucas Arantes Berg ◽  
Rafael Sachetto Oliveira ◽  
Rodrigo Weber dos Santos ◽  
Anders Nygren
Keyword(s):  
Single Cell ◽  
Cardiac Tissue ◽  
Cell Model ◽  
Membrane Resistance ◽  
Model Parameters ◽  
Single Cell Model

scholarly journals Direct observation of frequency modulated transcription in single cells using light activation

eLife ◽  
2013 ◽  
Vol 2 ◽  
Author(s):  
Daniel R Larson ◽  
Christoph Fritzsch ◽  
Liang Sun ◽  
Xiuhau Meng ◽  
David S Lawrence ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Molecule ◽  
Single Cell Analysis ◽  
Cell Model ◽  
Single Cells ◽  
Single Gene ◽  
Gene Activation ◽  
Light Activation ◽  
Dynamic Synthesis ◽  
Single Cell Model

Single-cell analysis has revealed that transcription is dynamic and stochastic, but tools are lacking that can determine the mechanism operating at a single gene. Here we utilize single-molecule observations of RNA in fixed and living cells to develop a single-cell model of steroid-receptor mediated gene activation. We determine that steroids drive mRNA synthesis by frequency modulation of transcription. This digital behavior in single cells gives rise to the well-known analog dose response across the population. To test this model, we developed a light-activation technology to turn on a single steroid-responsive gene and follow dynamic synthesis of RNA from the activated locus.

Sequence of Single Neuron Changes in CA1 Hippocampus of Rabbits During Acquisition of Trace Eyeblink Conditioned Responses

1997 ◽  
Vol 78 (2) ◽  
pp. 1030-1044 ◽  
Author(s):  
Matthew D. McEchron ◽  
John F. Disterhoft
Keyword(s):  
Single Neuron ◽  
Pyramidal Cells ◽  
Trace Conditioning ◽  
Neuron Activity ◽  
Inhibitory Influence ◽  
Temporal Properties ◽  
Response Profiles ◽  
Conditioned Responses ◽  
Ca1 Area ◽  
Nictitating Membrane Response

McEchron, Matthew D. and John F. Disterhoft. Sequence of single neuron changes in CA1 hippocampus of rabbits during acquisition of trace eyeblink conditioned responses. J. Neurophysiol. 78: 1030–1044, 1997. The sequence of changes in single neuron activity in the CA1 area of the rabbit hippocampus was examined during daily sessions (80 trials/session) of hippocampally dependent nonspatial trace eyeblink (i.e., nictitating membrane response) conditioning. Each trial for trace conditioned animals ( n = 7) consisted of a tone conditioned stimulus (CS; 6 kHz; 90 dB, 100 ms) followed by a 500-ms silent trace period, then a corneal airpuff unconditioned stimulus (US; 3.0 psi; 150 ms). Control animals( n = 5) received unpaired CSs and USs. Most pyramidal ( n = 309) and theta ( n = 21) cells were recorded for a single day of training. The activity of cells for each animal were grouped according to: the day of training that CRs began to increase and the day of training that CR performance became asymptotic. Pyramidal cells from trace conditioned animals demonstrated several stages of learning-related activity: large increases in activity after both the CS and US early in conditioning on the day of training when CRs began to increase, smaller moderate increases in activity on the following days of training, and decreases in activity after the US during asymptotic CRs. Pyramidal cell-increases declined significantly across the trials of each daily session. Theta cells showed an activity pattern opposite to the pyramidal cells, consistent with the notion that theta cells have an inhibitory influence on pyramidal cells. Single pyramidal cells also were categorized into response profiles. Most pyramidal response profiles showed increases in activity specific to the day of initial CRs. Two of the pyramidal response profiles may be involved in assessing the temporal properties of the CS-US trace conditioning trial.

scholarly journals Functional analysis of the methyltransferase SMYD in the single-cell model organism Tetrahymena thermophila

2020 ◽  
Vol 2 (2) ◽  
pp. 109-122
Author(s):  
Xiaolu Zhao ◽  
Yuan Li ◽  
Lili Duan ◽  
Xiao Chen ◽  
Fengbiao Mao ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Single Cell ◽  
Tetrahymena Thermophila ◽  
Cell Model ◽  
Model Organism ◽  
Single Cell Model
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