scholarly journals Study of the β-Cyclodextrin Imipramine Hydrochloride Inclusion Complex and Determination of its Stability Constant (K) by UV-Visible Spectroscopy

2007 ◽  
Vol 4 (4) ◽  
pp. 550-558 ◽  
Author(s):  
Alamdar Ashnagar ◽  
Nahid Gharib Naseri ◽  
Bita Khanaki
Keyword(s):  
Stability Constant ◽  
Inclusion Complex ◽  
Visible Spectroscopy ◽  
Constant Amount ◽  
A Value ◽  
Visible Spectra ◽  
Imipramine Hydrochloride ◽  
Uv Visible ◽  
The Stability

In this research, the interactions of imipramine hydrochloride drug with β- cyclodextrin and the stability constant (K) of the inclusion complex formed between them were investigated by using UV-visible spectroscopy. Solutions consisting of a known and constant amount of imipramine hydrochloride and varying amounts of β- cyclodextrin were prepared in 0.1 M phosphate buffer (pH 7.4). The final solutions had cyclodextrin concentrations between 0.0011 and 0.0153 M. UV-visible spectra of each solution was taken at λmax= 250 nm. The absorbances at this wavelength were recorded and plotted against cyclodextrin concentrations. From the graph, the concentrations of free and bound imipramine hydrochloride and free β-cyclodextrin were calculated using the Beer-Lambert law. From these data, the stability constant was calculated and a value of K=52.26±11.41 mol-1L was obtained. The magnitude of the stability constant is discussed in terms of the relative sizes and the chemical natures of β-cyclodextrin and imipramine hydrochloride.

Synthesis and biological analysis of anti-addiction effect and hepatotoxicity of tow baclofen analogues complexed with β-Cyclodextrin

2020 ◽  
Vol 23 ◽  
Author(s):  
Assia Keniche ◽  
Ibtissem EL Ouar ◽  
Ibtissem Zeghina ◽  
Mohammed El Amine Dib
Keyword(s):  
Stability Constant ◽  
Inclusion Complex ◽  
Alcohol Dependence ◽  
Biological Effects ◽  
Anxiolytic Effect ◽  
Excessive Consumption ◽  
The Stability ◽  
Job's Method

Aim and Objective : The excessive consumption of alcohol and the installation of a dependence is, in most cases, facilitated by favorable psychological factors which trigger and maintain the behavior of consumption. Examples more frequently encountered in individuals having difficulty with alcohol are in particular: one or more anxiety disorders, deficits in the capacities to manage stress and anxiety. The main objective of this work was study in vivo the anti-addiction effect and hepatotoxicity of tow baclofen analogues complexed with β-Cyclodextrin (βCD) on an alcohol-dependent rat model. Materials and Methods: The synthesis of two analogues ABF1 and ABF2 close to baclofen was reported. The structural determination of the two compounds was confirmed by NMR and IR analysis. The complexation of analogues with βCyclodextrin (βCD) was performed in water at room temperature (25 °C). The interactions of ABF with β-Cyclodextrin, and the stability constant (Ka) of the inclusion complex formed between them were investigated by using UV-visible spectroscopy. The biological effects of baclofen and the two analogues on alcohol dependence were studied in wistar rats. The anti-addiction effect of the analogues was tested by measuring the alcohol intake and the variation of the animal behaviour. The toxicity of the compounds was also analysed on liver injury markers. Results: The amino-3-phenylbutanoic acid (ABF1) and 3,4,5-trihydroxy-N-(methyl-2-acetate) benzamide (ABF2) were synthesized. The complexation of both analogues of baclofen (BF) with β-cyclodextrin (βCD) (ABF- βCD) was realized and confirmed by the stability constant of the inclusion complex (Ka) and Job’s method. The evaluation of anti-addiction activity in vivo showed that ABF1-βCD inhibits the consumption of alcohol at the doses equivalent to those of baclofen. Both baclofen analogues have shown an anxiolytic effect. Regarding the toxicity of the two compounds, our results showed that ABF1-βCD has less toxic effect than baclofen, it reduces the activity of ALT and AST enzymes. Histologically ABF1-βCD has no effect on structure of the liver in addition and has a protective effect against lesions alcohol-induced liver disease. Conclusion: Therefore, it can be suggested that ABF1 analogue combined with β-Cyclodextrin can be used as a treatment for alcohol dependence. Further clinical works are needed to confirm its effectiveness.

scholarly journals Azocalix[4]arene with three distal ethyl ester residues as a highly selective chromogenic sensor for Ca2+ ions

2018 ◽  
Vol 24 (3) ◽  
pp. 147-150
Author(s):  
Jie Wang ◽  
Hongyu Guan ◽  
Chunhua Ge ◽  
Ping Fan ◽  
Xijuan Xing ◽  
...  
Keyword(s):  
Stability Constant ◽  
Metal Ions ◽  
Ethyl Ester ◽  
Metal Ion ◽  
Visible Spectroscopy ◽  
Binding Properties ◽  
Metal Ion Complexation ◽  
Specific Selectivity ◽  
Uv Visible ◽  
The Stability

AbstractThree azocalix[4]arenes with distal ethyl ester residues, 5-phenylazo-25,26,27-tris[(ethoxycarbonyl)methoxy]-28-hydroxycalix[4]arene (2), 5-(o-methylphenyl)azo-25,26,27-tris[(ethoxycarbonyl)methoxy]-28-hydroxycalix[4]arene (3), 5-(p-Methylphenyl)azo-25,26,27-tris[(ethoxycarbonyl)methoxy]-28-hydroxycalix[4]arene (4), were synthesized and their binding properties with metal ions were investigated by ultraviolet (UV)/visible spectroscopy. The chromogenic behavior of these compounds upon metal ion complexation indicates a specific selectivity toward Ca2+ ion in the presence of other cations tested. The stoichiometry of 3 to Ca2+ ion in the complex is 1:1 and the stability constant of the complex is 1.28×104m−1.

Synthesis and solvatochromic studies of 2- amino-3-phenolazo1-(4-sulfophenyl)-3-methyi-5-pyrozolone and use it for the determination of trace amount of Nickel (II) in blood samples

2016 ◽  
Vol 5 (10) ◽  
pp. 4920
Author(s):  
Amar M. Ali ◽  
Hussain. J. Mohammed*
Keyword(s):  
Stability Constant ◽  
Trace Amount ◽  
Benzenesulfonic Acid ◽  
Determination Method ◽  
Blood Samples ◽  
Determination Of Nickel ◽  
Normal Human ◽  
The Stability ◽  
Normal Human Blood

A new, simple, sensitive and rapid spectrophotometric method is proposed for the determination of trace amount of Nickel (II). The method is based on the formation of a 1:2 complex with 4-(4-((2-hydroxy-6-nitrophenyl) diazenyl) -3-methyl-5-oxo-2, 5-dihydro-1H-pyrazol-1-yl) benzenesulfonic acid (2-ANASP) as a new reagent is developed. The complex has a maximum absorption at 516 nm and εmax of 1. 84 X 105 L. mol-1. cm-1. A linear correlation (0. 25 – 4. 0μg. ml-1) was found between absorbance at λmax and concentration. The accuracy and reproducibility of the determination method for various known amounts of Nickel (II) were tested. The results obtained are both precise (RSD was 1. 2 %) and accurate (relative error was 0. 787 %). The effect of diverse ions on the determination of Nickel (II) to investigate the selectivity of the method were also studied. The stability constant of the product was 0. 399 X 106 L. mol-1. The proposed method was successfully applied to the analysis of diabetes blood and normal human blood. 

Determination of the Stability Constant of the Calcium Dioxalate Complex

Urolithiasis ◽  
1981 ◽  
pp. 447-451
Author(s):  
John R. Burns ◽  
Birdwell Finlayson ◽  
Arthur Smith
Keyword(s):  
Stability Constant ◽  
The Stability

scholarly journals New Spectrophotometric Method for Determination of Cadmium

2009 ◽  
Vol 6 (s1) ◽  
pp. S496-S500
Author(s):  
K. S. Parikh ◽  
R. M. Patel ◽  
K. N. Patel
Keyword(s):  
Stability Constant ◽  
Spectrophotometric Method ◽  
Basic Medium ◽  
Buffer Solution ◽  
Solution Ph ◽  
Yellow Colour ◽  
Maximum Absorbance ◽  
The Stability

The reagent 2-hydroxy-4-n-butoxy-5-bromopropiophenone thiosemicarbazone (HBBrPT) has been used for the determination of Cd(II) by using spectrophotometric method. The reagent HBBrPT gave an intense yellow colour with Cd(II) solution in basic medium. The maximum absorbance was observed at 440 nm, in basic buffer solution (pH 10.00). The molor absorptivity and Sandell’s sensitivity of Cd(II)-HBBrPT complex were 4035 mol-1cm-1and 0.02765 μg cm-2respectively. The stability constant of 1:2 Cd(II)-HBBrPT complex was 8.46×106. The effect of various iron was also studied.

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