scholarly journals Correlation of Plasma MMP-1 and TIMP-1 Levels and the Colonic Mucosa Expressions in Patients with Ulcerative Colitis

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Ying-De Wang ◽  
Xiao-Yan Tan ◽  
Ke Zhang
Keyword(s):  
Ulcerative Colitis ◽  
Disease Severity ◽  
Plasma Levels ◽  
Colonic Mucosa ◽  
Venous Blood ◽  
Normal Subjects ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Reverse Transcription Pcr ◽  
Matrix Metalloproteinase 1 ◽  
The Relationship

Background. Both plasma and mucosal levels of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) have been shown to be independently correlated with ulcerative colitis (UC), but their relationship with each other and to disease severity remains unclear. This study aims to evaluate the relationship between colonic mucosal and plasma levels of MMP-1 and TIMP-1 with each other and with the severity of ulcerative colitis (UC).Methods. Colonic mucosal lesions and venous blood samples were collected from 30 patients with UC and 15 normal subjects. Real-time reverse transcription-PCR and immunohistochemistry were used to determine colonic mucosal MMP-1 and TIMP-1 expression; ELISA was used to measure plasma levels of MMP-1 and TIMP-1.Results. Expression of colonic mucosal and plasma MMP-1 and TIMP-1 in patients with UC was significantly higher than that of controls (P<.05), and was positively correlated with disease severity (P<.05). Plasma MMP-1 and TIMP-1 levels were well correlated with their corresponding expression in colonic mucosa (P<.05,r=0.805 and 0.908).Conclusion. Plasma MMP-1 and TIMP-1 levels reflect their colonic mucosal expression to some extent in patients with UC. Plasma MMP-1 and TIMP-1, in particular, demonstrate the potential to become biomarkers to clinically diagnose UC, predict its severity, and guide further therapy.

Dependence of Transcutaneous Oxygen Tension on Local Arteriovenous Pressure Gradient in Normal Subjects

1981 ◽  
Vol 60 (5) ◽  
pp. 499-506 ◽  
Author(s):  
C. R. Wyss ◽  
F. A. Matsen ◽  
Racheal V. King ◽  
C. W. Simmons ◽  
E. M. Burgess
Keyword(s):  
Oxygen Tension ◽  
Pressure Difference ◽  
Venous Blood ◽  
Venous Drainage ◽  
Normal Subjects ◽  
External Pressure ◽  
Transcutaneous Oxygen Tension ◽  
Transcutaneous Oxygen ◽  
Normal Men ◽  
The Relationship

1. We studied the relationship between trans-cutaneous oxygen tension at the foot and local arteriovenous pressure difference in 15 normal men and women; arteriovenous pressure difference was varied by changing the height of the foot with respect to the heart and by applying external pressure to the foot. 2. Control transcutaneous oxygen tension was 67 ± 9 sd mmHg (8.9 ± 1.2 kPa) at a control arteriovenous pressure difference of 80 ± 6 sd mmHg (10.6 ± 0.8 kPa). 3. In every subject transcutaneous oxygen tension fell non-linearly with a decrease in arteriovenous pressure difference; transcutaneous oxygen tension was relatively insensitive to changes in arteriovenous pressure difference when arteriovenous pressure difference was high, but always fell sharply to zero at some positive arteriovenous pressure difference [range 13-34 mmHg (1.7-4.5 kPa)]. 4. An analysis of the data indicated that transcutaneous oxygen tension varied with arteriovenous pressure difference approximately as the oxygen tension of cutaneous venous blood under the sensor varied (in the absence of changes in cutaneous vascular resistance and oxygen consumption). 5. This analysis was supported by studies in three subjects in whom the oxygen tension of superficial venous drainage from a warmed hand or foot was measured along with Transcutaneous oxygen tension while arteriovenous pressure difference was varied.

Effects of water immersion on sympathoadrenal and dopa-dopamine systems in humans

1992 ◽  
Vol 262 (6) ◽  
pp. R993-R999 ◽  
Author(s):  
E. Grossman ◽  
D. S. Goldstein ◽  
A. Hoffman ◽  
I. R. Wacks ◽  
M. Epstein
Keyword(s):  
Urinary Excretion ◽  
Plasma Levels ◽  
Water Immersion ◽  
Venous Blood ◽  
Urine Volume ◽  
Normal Subjects ◽  
Urinary Sodium Excretion ◽  
Peptide System ◽  
Triphasic Pattern ◽  
Atrial Natriuretic

Water immersion to the neck increases central blood volume and evokes a marked diuresis and natriuresis. The present study examined simultaneously effects of water immersion on activities of three endogenous systems thought to participate in sodium homeostasis: the sympathetic nervous system, the atrial natriuretic peptide system, and the renal dopa-dopamine system. Hourly urine collections and antecubital venous blood samples were obtained from 10 normal subjects before, during, and after sitting in a water-immersion tank for 3 h; four control subjects were studied while seated without immersion. Urine volume was increased by more than threefold after 1 h of immersion (from 1.2 +/- 0.2 ml/min at baseline to 5.9 +/- 0.7 ml/min, P less than 0.001) and peaked during the second hour. Urinary sodium excretion increased by more than twofold (from 103 +/- 17 mu eq/min at baseline to 196 +/- 36 mu eq/min at 1 h, P less than 0.001) and peaked during the third hour. Plasma levels and urinary excretion of norepinephrine (NE) and epinephrine were suppressed consistently during immersion (P less than 0.05). There was a marked, prompt, and sustained increase in plasma levels of immunoreactive atrial natriuretic factor (irANF) from 6.9 +/- 1.9 pg/ml baseline to 17.3 +/- 4.3 pg/ml at 1 h (P less than 0.001). Urinary excretion of dopa, dopamine, and 3,4-dihydroxyphenylglycol, a neuronal metabolite of NE, changed in a triphasic pattern, with decreased excretion during the first hour of immersion (P less than 0.01), small but consistent increases during the next 2 h, and decreased excretion, to below baseline, during recovery (P less than 0.01 for dopa and dopamine).(ABSTRACT TRUNCATED AT 250 WORDS)

Colonic mucosa‐associated candida assessed by biopsy culture is associated with disease severity in ulcerative colitis: A prospective study

2019 ◽  
Vol 20 (12) ◽  
pp. 642-648 ◽  
Author(s):  
Jimil Shah ◽  
Usha Dutta ◽  
Shivaprakash Rudramurthy ◽  
Arunaloke Chakrabarti ◽  
Saroj K. Sinha ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Prospective Study ◽  
Disease Severity ◽  
Colonic Mucosa ◽  
A Prospective Study

scholarly journals CYP3A5 Genotype as a Potential Pharmacodynamic Biomarker for Tacrolimus Therapy in Ulcerative Colitis in Japanese Patients

2020 ◽  
Vol 21 (12) ◽  
pp. 4347
Author(s):  
Yuki Yamamoto ◽  
Hiroshi Nakase ◽  
Minoru Matsuura ◽  
Shihoko Maruyama ◽  
Satohiro Masuda
Keyword(s):  
Ulcerative Colitis ◽  
Mrna Expression ◽  
Therapeutic Effect ◽  
Blood Concentration ◽  
Colonic Mucosa ◽  
Cyp3a5 Genotype ◽  
Tacrolimus Therapy ◽  
The Relationship ◽  
Mrna Expression Levels ◽  
Cyp3a5 Polymorphism

Tacrolimus has been used to induce remission in patients with steroid-refractory ulcerative colitis. It poses a problem of large individual differences in dosage necessary to attain target blood concentration and, often, this leads to drug inefficacy. We examined the difference in mRNA expression levels of ATP binding cassette transporter B1 (ABCB1) between inflamed and non-inflamed tissues, and the influence of CYP3A5 genotype on tacrolimus therapy. The mRNA expression of CYP3A4 in colonic mucosa and that of cytochrome p450 3A5 (CYP3A5) and ABCB1 in inflamed and non-inflamed areas were examined in 14 subjects. The mRNA expression levels of CYP3A5 were higher than that of CYP3A4. The mRNA expression of ABCB1 was lower in the inflamed than in the non-inflamed mucosa, despite that of CYP3A5 mRNA level being not significantly changed. Hence, the deterioration of the disease is related to the reduction of the barrier in the inflamed mucosa. The relationship between CYP3A5 genotype and blood concentration, dose, and concentration/dose (C/D) ratio of tacrolimus in 15 subjects was studied. The tacrolimus dose to maintain equivalent blood concentrations was lower in CYP3A5*3/*3 than in CYP3A5*1 carriers, and the C/D ratio was significantly higher in the latter. Thus, CYP3A5 polymorphism information played a role in determining the initial dose of tacrolimus. Furthermore, since the effect of tacrolimus appears earlier in CYP3A5*3/*3 than in CYP3A5*1/*1 and *1/*3, it seems necessary to change the evaluation time of therapeutic effect by CYP3A5 genotype. Additionally, the relationship between CYP3A5 genotype and C/D ratio of tacrolimus in colonic mucosa was investigated in 10 subjects. Tacrolimus concentration in the mucosa was two-fold higher in CYP3A5*3/*3 than in CYP3A5*1 carriers, although no significant difference in tacrolimus-blood levels was observed. Therefore, the local concentration of tacrolimus affected by CYP3A5 polymorphism might be related to its therapeutic effect.

scholarly journals Plasma matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 as prognostic biomarkers in critically ill patients

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 50-56
Author(s):  
Izabela Duda ◽  
Łukasz Krzych ◽  
Halina Jędrzejowska-Szypułka ◽  
Joana Lewin-Kowalik
Keyword(s):  
Matrix Metalloproteinase ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Plasma Levels ◽  
Matrix Metalloproteinase 9 ◽  
Prognostic Biomarkers ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Tissue Inhibitor ◽  
Matrix Metalloproteinase 1 ◽  
Metalloproteinase 9

AbstractMatrix metalloproteinase 9 (MMP-9) plays an important role in inflammatory and pathological processes by enabling the inflow of leukocytes to the site of infection or tissue damage. MMP-9 and tissue inhibitor of metalloproteinase 1 (TIMP-1) have been described as potential prognostic biomarkers in various clinical settings. The aim of the study was to evaluate the usefulness of plasma levels of MMP-9 and TIMP-1 as well as the MMP-9/ TIMP-1 ratio in predicting the outcome in patients admitted to the intensive care unit (ICU). The study included 56 critically ill patients with multiple organ failure. Plasma levels of MMP-9 and TIMP-1 were determined on hospitalization day 1, 2, 3 and 7. Nineteen (35.7%) patients died. The level of TIMP-1 was statistically significantly higher on day 1 and 7 of hospitalization in non-survivors, as compared to survivors (p=0.01). A statistically significant positive correlation was found between MMP-9 and TIMP-1. The MMP-9/TIMP-1 ratio was comparable in both groups during of observation (0.62 on day 1). The MMP-9/TIMP-1 ratio was positively correlated with the level of lactate and negatively correlated with platelet count. Likewise, TIMP-1 was positively correlated with the level of lactate. The level of MMP-9 was higher in the non-survivor group only on day 7 of observation. In conclusion, although TIMP-1 and MMP-9 concentrations were higher in non-survivors and the MMP-9/TIMP-1 ratio was related to some parameters of critical illness, further research is needed to verify whether they can serve as reliable biomarkers for early prognostication of ICU patients.

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