scholarly journals Lowering Caveolin-1 Expression in Human Vascular Endothelial Cells Inhibits Signal Transduction in Response to Shear Stress

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
A. D. van der Meer ◽  
M. M. J. Kamphuis ◽  
A. A. Poot ◽  
J. Feijen ◽  
I. Vermes
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Human Endothelial Cells ◽  
Caveolin 1 ◽  
Low Levels ◽  
Endothelial Growth Factor

Vascular endothelial cells have an extensive response to physiological levels of shear stress. There is evidence that the protein caveolin-1 is involved in the early phase of this response. In this study, caveolin-1 was downregulated in human endothelial cells by RNAi. When these cells were subjected to a shear stress of 15 dyn/cm2for 10 minutes, activation of Akt and ERK1/2 was significantly lower than in control cells. Moreover, activation of Akt and ERK1/2 in response to vascular endothelial growth factor was significantly lower in cells with low levels of caveolin-1. However, activation of integrin-mediated signaling during cell adhesion onto fibronectin was not hampered by lowered caveolin-1 levels. In conclusion, caveolin-1 is an essential component in the response of endothelial cells to shear stress. Furthermore, the results suggest that the role of caveolin-1 in this process lies in facilitating efficient VEGFR2-mediated signaling.

scholarly journals Shear stress augments mitochondrial ATP generation that triggers ATP release and Ca2+ signaling in vascular endothelial cells

2018 ◽  
Vol 315 (5) ◽  
pp. H1477-H1485 ◽  
Author(s):  
Kimiko Yamamoto ◽  
Hiromi Imamura ◽  
Joji Ando
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Vascular Endothelial Cells ◽  
Critical Role ◽  
Atp Release ◽  
Vascular Endothelial ◽  
The Novel ◽  
Caveolin 1 ◽  
Atp Generation

Vascular endothelial cells (ECs) sense and transduce hemodynamic shear stress into intracellular biochemical signals, and Ca2+ signaling plays a critical role in this mechanotransduction, i.e., ECs release ATP in the caveolae in response to shear stress and, in turn, the released ATP activates P2 purinoceptors, which results in an influx into the cells of extracellular Ca2+. However, the mechanism by which the shear stress evokes ATP release remains unclear. Here, we demonstrated that cellular mitochondria play a critical role in this process. Cultured human pulmonary artery ECs were exposed to controlled levels of shear stress in a flow-loading device, and changes in the mitochondrial ATP levels were examined by real-time imaging using a fluorescence resonance energy transfer-based ATP biosensor. Immediately upon exposure of the cells to flow, mitochondrial ATP levels increased, which was both reversible and dependent on the intensity of shear stress. Inhibitors of the mitochondrial electron transport chain and ATP synthase as well as knockdown of caveolin-1, a major structural protein of the caveolae, abolished the shear stress-induced mitochondrial ATP generation, resulting in the loss of ATP release and influx of Ca2+ into the cells. These results suggest the novel role of mitochondria in transducing shear stress into ATP generation: ATP generation leads to ATP release in the caveolae, triggering purinergic Ca2+ signaling. Thus, exposure of ECs to shear stress seems to activate mitochondrial ATP generation through caveola- or caveolin-1-mediated mechanisms. NEW & NOTEWORTHY The mechanism of how vascular endothelial cells sense shear stress generated by blood flow and transduce it into functional responses remains unclear. Real-time imaging of mitochondrial ATP demonstrated the novel role of endothelial mitochondria as mechanosignaling organelles that are able to transduce shear stress into ATP generation, triggering ATP release and purinoceptor-mediated Ca2+ signaling within the cells.

scholarly journals Crucial Role of Inhibitor of DNA Binding/Differentiation in the Vascular Endothelial Growth Factor-Induced Activation and Angiogenic Processes of Human Endothelial Cells

2004 ◽  
Vol 173 (9) ◽  
pp. 5801-5809 ◽  
Author(s):  
Daisuke Sakurai ◽  
Naoyuki Tsuchiya ◽  
Akihiro Yamaguchi ◽  
Yurai Okaji ◽  
Nelson H. Tsuno ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Dna Binding ◽  
Crucial Role ◽  
Vascular Endothelial ◽  
Human Endothelial Cells ◽  
Inhibitor Of Dna Binding ◽  
Endothelial Growth Factor

scholarly journals Crucial Role of Inhibitor of DNA Binding/Differentiation in the Vascular Endothelial Growth Factor-Induced Activation and Angiogenic Processes of Human Endothelial Cells.

2005 ◽  
Vol 174 (6) ◽  
pp. 3818.2-3818
Author(s):  
Daisuke Sakurai ◽  
Naoyuki Tsuchiya ◽  
Akihiro Yamaguchi ◽  
Yurai Okaji ◽  
Nelson H. Tsuno ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Dna Binding ◽  
Crucial Role ◽  
Vascular Endothelial ◽  
Human Endothelial Cells ◽  
Inhibitor Of Dna Binding ◽  
Endothelial Growth Factor

scholarly journals Antisense to Cyclin D1 Inhibits Vascular Endothelial Growth Factor–Stimulated Growth of Vascular Endothelial Cells: Implication of Tumor Vascularization

2006 ◽  
Vol 12 (15) ◽  
pp. 4720-4729 ◽  
Author(s):  
Masayoshi Yasui ◽  
Hirofumi Yamamoto ◽  
Chew Yee Ngan ◽  
Bazarragchaa Damdinsuren ◽  
Yurika Sugita ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Cyclin D1 ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Tumor Vascularization ◽  
Endothelial Growth Factor

scholarly journals The Role of Vascular Endothelial Growth Factor-Induced Activation of NADPH Oxidase in Choroidal Endothelial Cells and Choroidal Neovascularization

2010 ◽  
Vol 177 (4) ◽  
pp. 2091-2102 ◽  
Author(s):  
Elizabeth Monaghan-Benson ◽  
John Hartmann ◽  
Aleksandr E. Vendrov ◽  
Steve Budd ◽  
Grace Byfield ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Nadph Oxidase ◽  
Choroidal Neovascularization ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor
Sign in / Sign up

Export Citation Format

Share Document