Fecal Occult Blood Test and Gastrointestinal Parasitic Infection

Stool specimens of 1238 workers in western region of Saudi Arabia were examined for infection with intestinal parasites and for fecal occult blood (FOB) to investigate the possibility that enteroparasites correlate to occult intestinal bleeding. Direct smears and formal ether techniques were used for detection of diagnostic stages of intestinal parasites. A commercially available guaiac test was used to detect fecal occult blood. 47.01% of the workers were infected with intestinal parasites including eight helminthes species and eight protozoan species. The results provided no significant evidence (P-value=0.143) that intestinal parasitic infection is in association with positive guaiac FOB test.

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Monoclonal Antibodies for Exercise-Induced Fecal Blood Detection-Comparison With Hemofec

Canadian Journal of Applied Physiology ◽

10.1139/h95-006 ◽

1995 ◽

Vol 20 (1) ◽

pp. 78-88

Author(s):

Cristina Yges ◽

José L. Chicharro ◽

Alejandro Lucía ◽

Julio C. Legido ◽

Fernando Bandrés

Keyword(s):

Monoclonal Antibodies ◽

Gastrointestinal Bleeding ◽

Occult Blood ◽

Fecal Occult Blood ◽

The Third ◽

Fecal Occult ◽

Qualitative Test ◽

Stool Specimens ◽

Exercise Induced ◽

Key Words Gastrointestinal

The purpose of this investigation was to determine the incidence of fecal occult blood in marathoners using an immunochemical technique (OC-Hemodia). Five stool specimens (2 pre- and 3 postrace) were collected from 24 male runners (mean age 41.4 ± 9.3 yrs) and analysed for fecal occult blood using the OC-Hemodia test. The results were also compared with a qualitative test (Hemofec) in 12 subjects who were randomly selected from the overall group of 24 runners. With the immunochemical technique, the results evidenced the presence of fecal occult blood in 8 subjects in the first postrace stool specimens. Four of these 8 subjects also tested positive in the second postrace sample, whereas in the third postrace sample only one of them tested positive. With the qualitative test, fecal blood was demonstrated in 10 runners in the first postrace sample. Eight of them tested positive in the second sample, whereas only 5 tested positive in the third sample. The immunochemical technique is recommended for fecal occult blood detection in marathoners. Key words: gastrointestinal bleeding, immunochemical technique, marathon

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Spectrophotometry of occult blood in feces.

Clinical Chemistry ◽

10.1093/clinchem/29.12.2022 ◽

1983 ◽

Vol 29 (12) ◽

pp. 2022-2025 ◽

Cited By ~ 8

Author(s):

C L Welch ◽

D S Young

Keyword(s):

Ascorbic Acid ◽

Limit Of Detection ◽

Occult Blood ◽

Fecal Occult Blood ◽

Fecal Occult ◽

Analytical Recovery ◽

Wet Weight ◽

Stool Specimens ◽

Dye Oxidation ◽

Catalyzed Oxidation

Abstract This method for measuring fecal occult blood is based on the heme-catalyzed oxidation of tetramethylbenzidine by H2O2. An aliquot of heated stool homogenate is mixed with acetic acid to chemically separate heme from globin. The heme is extracted into ethyl acetate and reacted with the reagent and H2O2 to produce a green oxidation product. The reaction is followed kinetically for 30 to 60 s at 660 nm. A660 is linearly related to the amount of hemoglobin. The lower limit of detection is 1 to 2 mg of hemoglobin per gram (wet weight) of feces. Within-day precision (CV) of the analysis for hemoglobin added to stool specimens (4 to 30 mg/g) ranged from 2.3 to 7.6%, between-day CV from 2.1 to 8.1%. Analytical recovery of hemoglobin added to fecal specimens (4 to 30 mg/g) ranged from 86.7 to 106.2%. Of the substances known to interfere with conventional dye-oxidation tests for fecal occult blood, only myoglobin and ascorbic acid interfere with hemoglobin quantification by our procedure. The test is fast, inexpensive, and easy to perform, and involves equipment available in hospital laboratories.

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Rapid immunochemical detection of fecal occult blood by use of a latex-agglutination test.

Clinical Chemistry ◽

10.1093/clinchem/34.9.1758 ◽

1988 ◽

Vol 34 (9) ◽

pp. 1763-1766 ◽

Cited By ~ 11

Author(s):

P Väänänen ◽

R Tenhunen

Keyword(s):

Occult Blood ◽

Latex Agglutination ◽

Fecal Occult Blood ◽

Special Equipment ◽

Fecal Occult ◽

Immunochemical Detection ◽

Human Myoglobin ◽

Immunochemical Test ◽

Stool Specimens ◽

Healthy Persons

Abstract A novel immunochemical test, "Hemolex," is characterized and compared with three guaiac tests: "Hemoccult" (Smith-Kline, U.S.A.), "Hemofec" (Boehringer Mannheim, F.R.G.), and "Fecatwin Sensitive" (Labsystems, Finland). We tested 191 stool specimens from patients, 144 from healthy persons. The sensitivity of Hemolex was 0.6 mL of blood per 100 g of stool. It reacts with neither animal hemoglobin nor human myoglobin, unlike the guaiac tests, nor does it react with the stool of healthy persons under no dietary restrictions, while the guaiac tests react variably. As a rule, Hemolex is insensitive to bleeding in the upper gastrointestinal tract, unlike the guaiac tests. However, Hemolex seems to be more sensitive to bleeding in the lower gastrointestinal canal than are the other tests, and we show it to be suitable for screening for occult blood originating there. It is unaffected by diet. It is simple to perform, requires no special equipment, and stool specimens, stored in the test diluent, are stable for testing for two weeks.

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