scholarly journals Characterization of Plasmid pPO1 from the HyperacidophilePicrophilus oshimae

Archaea ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Angel Angelov ◽  
Jörn Voss ◽  
Wolfgang Liebl
Keyword(s):  
Ph Optimum ◽  
Modification System ◽  
Moderately Thermophilic ◽  
E Coli ◽  
Restriction Modification System ◽  
Picrophilus Torridus ◽  
Transcriptional Start Sites ◽  
Native Host ◽  
Restriction Modification

Picrophilus oshimaeandPicrophilus torridusare free-living, moderately thermophilic and acidophilic organisms from the lineage ofEuryarchaeota. With a pH optimum of growth at pH 0.7 and the ability to even withstand molar concentrations of sulphuric acid, these organisms represent the most extreme acidophiles known. So far, nothing is known about plasmid biology in these hyperacidophiles. Also, there are no genetic tools available for this genus. We have mobilized the 7.6 Kbp plasmid fromP. oshimaeinE. coliby introducing origin-containing transposons and described the plasmid in terms of its nucleotide sequence, copy number in the native host, mode of replication, and transcriptional start sites of the encoded ORFs. Plasmid pPO1 may encode a restriction/modification system in addition to its replication functions. The information gained from the pPO1 plasmid may prove useful in developing a cloning system for this group of extreme acidophiles.

Characterization of pPvu1, the autonomous plasmid from proteus vulgaris that carries the genes of the PvuII restriction-modification system

Gene ◽  
1995 ◽  
Vol 157 (1-2) ◽  
pp. 73-79 ◽  
Author(s):  
Michelle D. Calvin Koons ◽  
Robert M. Blumenthal
Keyword(s):  
Proteus Vulgaris ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

Cloning and characterization of the BamHI restriction modification system

Gene ◽  
1988 ◽  
Vol 74 (1) ◽  
pp. 13 ◽  
Author(s):  
J.E. Brooks ◽  
J.S. Benner ◽  
K.R. Silber ◽  
D.F. Heiter ◽  
L.A. Sznyter ◽  
...  
Keyword(s):  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

Characterization of pECL18 and pKPN2: a proposed pathway for the evolution of two plasmids that carry identical genes for a Type II restriction-modification system

2002 ◽  
Vol 267 (2) ◽  
pp. 171-178 ◽  
Author(s):  
M. Zakharova ◽  
I. Beletskaya ◽  
M. Denjmukhametov ◽  
T. Yurkova ◽  
L. Semenova ◽  
...  
Keyword(s):  
Type Ii ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

scholarly journals Characterization of the Type I Restriction Modification System Broadly Conserved among Group A Streptococci

mSphere ◽  
2021 ◽  
Author(s):  
Sruti DebRoy ◽  
William C. Shropshire ◽  
Chau Nguyen Tran ◽  
Haiping Hao ◽  
Marc Gohel ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Epigenetic Modification ◽  
Type I ◽  
Whole Genome ◽  
Group A Streptococci ◽  
Modification System ◽  
Restriction Modification System ◽  
Group A ◽  
Restriction Modification

The advent of whole-genome approaches capable of detecting DNA methylation has markedly expanded appreciation of the diverse roles of epigenetic modification in prokaryotic physiology. For example, recent studies have suggested that DNA methylation impacts gene expression in some streptococci.

scholarly journals Isolation of A Novel Bacillus thuringiensis Phage Representing A New Phage Lineage and Characterization of Its Endolysin

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 611 ◽  
Author(s):  
Yihui Yuan ◽  
Qin Peng ◽  
Shuo Yang ◽  
Shaowen Zhang ◽  
Yajuan Fu ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Pathogenic Bacteria ◽  
Evolutionary Relationship ◽  
Genomic Analysis ◽  
Host Bacterium ◽  
The Novel ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

Phages, the parasites of bacteria, are considered as a new kind of antimicrobial agent due to their ability to lyse pathogenic bacteria. Due to the increase of available phage isolates, the newly isolated phage showed increasing genomic similarities with previously isolated phages. In this study, the novel phage vB_BthS_BMBphi, infecting the Bacillus thuringiensis strain BMB171, is isolated and characterized together with its endolysin. This phage is the first tadpole-like phage infecting the Bacillus strains. Genomic analysis shows that the phage genome is dissimilar to all those of previously characterized phages, only exhibiting low similarities with partial regions of the B. thuringiensis prophages. Phylogenetic analysis revealed that the phage was distant from the other Bacillus phages in terms of evolution. The novel genome sequence, the distant evolutionary relationship, and the special virion morphology together suggest that the phage vB_BthS_BMBphi could be classified as a new phage lineage. The genome of the phage is found to contain a restriction modification system, which might endow the phage with immunity to the restriction modification system of the host bacterium. The function of the endolysin PlyBMB encoded by the phage vB_BthS_BMBphi was analyzed, and the endolysin could lyse all the tested Bacillus cereus group strains, suggesting that the endolysin might be used in controlling pathogenic B. cereus group strains. The findings of this study enrich the understanding of phage diversity and provide a resource for controlling the B. cereus group pathogenic bacteria.

Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli

Gene ◽  
1996 ◽  
Vol 172 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Hans-Martin Striebel ◽  
Stefan Seeber ◽  
Michael Jarsch ◽  
Christoph Kessler
Keyword(s):  
Escherichia Coli ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification
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