scholarly journals Optimization of Parameters for a More Efficient Use of Adipose-Derived Stem Cells in Regenerative Medicine Therapies

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Meire Aguena ◽  
Roberto Dalto Fanganiello ◽  
Luiz Alexandre Lorico Tissiani ◽  
Felipe Augusto André Ishiy ◽  
Rodrigo Atique ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Adipocyte Differentiation ◽  
Soft Tissue Reconstruction ◽  
Adipose Derived Stem Cells ◽  
Fat Tissue ◽  
Lower Abdomen ◽  
Optimization Of Parameters ◽  
Significant Harm ◽  
Tissue Reconstruction

Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is dependent on the number of ASCs. In a sample of 10 donors, we found a higher concentration of nucleated cells in the lower abdomen compared to flank (P=0.015). In a sample of 6 donors we did not find differences in the cell yield obtained by manual or pump-assisted aspiration (P=0.56). We suggest that the increase in the number of ASCs in the reinjected fat may enhance the efficiency of newly formed adipose tissue and that the anatomical region from which to harvest fat tissue needs to be considered to optimize the number of ASCs in the harvested tissue. Finally, pump-assisted aspiration can be used without any significant harm to the viability of cells.

scholarly journals Analysis of DYRK1B, PPARG, and CEBPB Expression Patterns in Adipose-Derived Stem Cells from Patients carrying DYRK1B R102C and Healthy Individuals During Adipogenesis

2021 ◽  
Author(s):  
Azam Armanmehr ◽  
Hossein Jafari Khamirani ◽  
Sina Zoghi ◽  
Mehdi Dianatpour
Keyword(s):  
Metabolic Syndrome ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Transcription Factors ◽  
Adipocyte Differentiation ◽  
Expression Patterns ◽  
Signs And Symptoms ◽  
Adipose Derived Stem Cells ◽  
Healthy Individuals ◽  
Normal Individuals

Background: Metabolic syndrome (MetS) is a group of signs and symptoms that are associated with a higher risk of Type 2 Diabetes Mellitus (T2DM) and Cardiovascular Diseases (CVDs). The major risk factor for developing MetS is abdominal obesity that is caused by an increase in adipocyte size or number. Adipocyte number multiplication is caused by the differentiation of mesenchymal stem cells into adipose tissue. Numerous studies have evaluated the expression of key transcription factors including PPARG and CEBPB during adipocyte differentiation in murine cells such as 3T3-L1 cell line. In order to comprehend the expression changes during the process of fat accumulation in adipose tissue derived stem cells (ASCs), we compared the expression of DYRK1B, PPARG, and CEBPB in undifferentiated and differentiated ASCs into mature adipocytes between the patient (harboring DYRK1b R102C) and control (healthy individuals) groups. Methods: Gene expression was evaluated on eighth days pre-induction and days 1, 5, and 15 post-induction. The pluripotent capacity of ASCs and the potential for differentiation into adipocytes were confirmed by flow cytometry analysis of surface markers (CD34, CD44, CD105, and CD90), and Oil red O staining, respectively. Expressions of DYRK1B, PPARG, and CEBPB were assessed by RT-PCR in patients' and normal individuals' samples. Results: The expression of DYRK1B kinase and transcription factors (CEBPB and PPARG) are significantly higher in adipose derived stem cells harboring DYRK1b R102C compared to non-carriers on days 5 and 15 during adipocyte differentiation. These proteins may be suitable targets for therapeutic strategies in obesity and obesity related disorders like metabolic syndrome. Furthermore, AZ191 exhibited a potent and selective inhibitory activity toward DYRK1B and CEBPB. Conclusion: CEBPB, PPARA, and DYRK1B contribute to adipogenesis and the development of metabolic syndrome; thus, they can be harnessed in developing therapeutic agents against metabolic syndrome.

scholarly journals MicroRNA-27 (miR-27) Targets Prohibitin and Impairs Adipocyte Differentiation and Mitochondrial Function in Human Adipose-derived Stem Cells

2013 ◽  
Vol 288 (48) ◽  
pp. 34394-34402 ◽  
Author(s):  
Ting Kang ◽  
Wan Lu ◽  
Wei Xu ◽  
Leonard Anderson ◽  
Methode Bacanamwo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mitochondrial Function ◽  
Adipocyte Differentiation ◽  
Adipose Derived Stem Cells

scholarly journals Hybrid Adipogenic Implants from Adipose Stem Cells for Soft Tissue Reconstruction In Vivo

2010 ◽  
Vol 16 (11) ◽  
pp. 3299-3307 ◽  
Author(s):  
Eduardo K. Moioli ◽  
Mo Chen ◽  
Rujing Yang ◽  
Bhranti Shah ◽  
June Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Soft Tissue ◽  
Adipose Stem Cells ◽  
Soft Tissue Reconstruction ◽  
Tissue Reconstruction

Adipose tissue engineering with naturally derived scaffolds and adipose-derived stem cells

Biomaterials ◽  
2007 ◽  
Vol 28 (26) ◽  
pp. 3834-3842 ◽  
Author(s):  
Lauren Flynn ◽  
Glenn D. Prestwich ◽  
John L. Semple ◽  
Kimberly A. Woodhouse
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Adipose Derived Stem Cells ◽  
Adipose Tissue Engineering

scholarly journals Assessment of human cartilage regeneration in a patient with knee osteoarthritis using autologous adipose-tissue-derived stem cells and Platelet-rich plasma: a case study

2020 ◽  
pp. 73-78
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Physical Therapy ◽  
Platelet Rich Plasma ◽  
Cartilage Regeneration ◽  
Adipose Derived Stem Cells ◽  
Human Cartilage ◽  
Percutaneous Injection ◽  
Knee Oa ◽  
Mri Scans

Cartilage regenerative medicine has been met with much interest due to their ability to inhibit disease progression of osteoarthritis (OA). The use of adipose-derived stem cells has been suggested as a reliable method for OA treatment because of their potential to differentiate into a variety of cell lines and their potent capability to self-renewal and repair. The aim of this study is to assess adipose-derived stem cells in combination with PRP ability in treating a patient with knee OA. A 53-year- old man with osteoarthritis was selected for this treatment. Human abdominal subcutaneous adipose sample was obtained from a patient with knee OA. Stem cells were obtained from adipose tissue of abdominal origin by digesting lipoaspirate tissue with collagenase. ADSCs cultured in DMEM medium supplemented with 10% FBS. Also, ADSCs expanded and characterized by flow cytometry. These stem cells, along with platelet-rich plasma and calcium chloride, were injected into the right knee. Pre-treatment and post-treatment MRI scans, physical therapy, and pain score data were then analyzed. The MRI data for the patient demonstrated significant positive changes. Probable cartilage regeneration was sensible in the patient. Along with MRI evidence, the measured physical therapy outcomes, subjective pain, and functional status all improved. Autologous adipose-derived stem cell injection, in conjunction with platelet-rich plasma is a promising minimally invasive therapy for osteoarthritis of human knees. The present clinical case report demonstrated that a combination of percutaneous injection of autologous ADSCs and PRPmay be able to regenerate cartilage in human knee OA.

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