scholarly journals Human Umbilical Cord Blood-Derived Mesenchymal Stem Cell Therapy Promotes Functional Recovery of Contused Rat Spinal Cord through Enhancement of Endogenous Cell Proliferation and Oligogenesis

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Sang In Park ◽  
Jung Yeon Lim ◽  
Chang Hyun Jeong ◽  
Seong Muk Kim ◽  
Jin Ae Jun ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Cell Proliferation ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Functional Recovery ◽  
Umbilical Cord Blood ◽  
Daily Injection ◽  
Control Group ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord

Numerous studies have shown the benefits of mesenchymal stem cells (MSCs) on the repair of spinal cord injury (SCI) model and on behavioral improvement, but the underlying mechanisms remain unclear. In this study, to investigate possible mechanisms by which MSCs contribute to the alleviation of neurologic deficits, we examined the potential effect of human umbilical cord blood-derived MSCs (hUCB-MSCs) on the endogenous cell proliferation and oligogenesis after SCI. SCI was injured by contusion using a weight-drop impactor and hUCB-MSCs were transplanted into the boundary zone of the injured site. Animals received a daily injection of bromodeoxyuridine (BrdU) for 7 days after treatment to identity newly synthesized cells of ependymal and periependymal cells that immunohistochemically resembled stem/progenitor cells was evident. Behavior analysis revealed that locomotor functions of hUCB-MSCs group were restored significantly and the cavity volume was smaller in the MSCs-transplanted rats compared to the control group. In MSCs-transplanted group, TUNEL-positive cells were decreased and BrdU-positive cells were significantly increased rats compared with control group. In addition, more of BrdU-positive cells expressed neural stem/progenitor cell nestin and oligo-lineage cell such as NG2, CNPase, MBP and glial fibrillary acidic protein typical of astrocytes in the MSC-transplanted rats. Thus, endogenous cell proliferation and oligogenesis contribute to MSC-promoted functional recovery following SCI.

scholarly journals Functional Recovery & Neurogenesis after Transplantation of Stem Cells from Human Umbilical Cord Blood into Spinal Cord Injured Rats

2013 ◽  
Vol 5 (1) ◽  
pp. 4-13
Author(s):  
Endegena Gemta ◽  
Amani El- Baz ◽  
Yasser El Wazir ◽  
Mohammed Abdo ◽  
Hassan Al-Shatoury ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Functional Recovery ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Spinal Cord Injured

The use of hemopoietic stem cells derived from human umbilical cord blood to promote restoration of spinal cord tissue and recovery of hindlimb function in adult rats

2006 ◽  
Vol 5 (5) ◽  
pp. 424-433 ◽  
Author(s):  
Yutaka Nishio ◽  
Masao Koda ◽  
Takahito Kamada ◽  
Yukio Someya ◽  
Katsunori Yoshinaga ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Functional Recovery ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Therapeutic Effects ◽  
Hemopoietic Stem Cells

Object The use of human umbilical cord blood (HUCB) cells has been reported to improve functional recovery in cases of central nervous system injuries such as stroke, traumatic brain injury, and spinal cord injury (SCI). The authors investigated the effects of hemopoietic stem cells that were derived from HUCB and transplanted into the injured spinal cords of rats. Methods One week after injury, an HUCB fraction enriched in CD34-positive cells was transplanted into the experimental group. In control animals, vehicle (Matrigel) was transplanted. Recovery of motor functions was assessed using the Basso, Beattie, and Bresnahan Locomotor Scale, and immunohistochemical examinations were performed. Cells from HUCB that were CD34 positive improved functional recovery, reduced the area of the cystic cavity at the site of injury, increased the volume of residual white matter, and promoted the regeneration or sparing of axons in the injured spinal cord. Immunohistochemical examination revealed that transplanted CD34-positive cells survived in the host spinal cord for at least 3 weeks after transplantation but had disappeared by 5 weeks. The transplanted cells were not positive for neural markers, but they were positive for hemopoietic markers. There was no evidence of an immune reaction at the site of injury in either group. Conclusions These results suggest that transplantation of a CD34-positive fraction from HUCB may have therapeutic effects for SCI. The results of this study provide important preclinical data regarding HUCB stem cell–based therapy for SCI.

scholarly journals Electrophysiological, Morphological, and Ultrastructural Features of the Injured Spinal Cord Tissue after Transplantation of Human Umbilical Cord Blood Mononuclear Cells Genetically Modified with the VEGF and GDNF Genes

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Y. O. Mukhamedshina ◽  
Z. E. Gilazieva ◽  
S. S. Arkhipova ◽  
L. R. Galieva ◽  
E. E. Garanina ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Mononuclear Cells ◽  
Genetically Modified ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Blood Mononuclear Cells ◽  
Cord Blood Mononuclear Cells

In this study, we examined the efficacy of human umbilical cord blood mononuclear cells (hUCB-MCs), genetically modified with the VEGF and GDNF genes using adenoviral vectors, on posttraumatic regeneration after transplantation into the site of spinal cord injury (SCI) in rats. Thirty days after SCI, followed by transplantation of nontransduced hUCB-MCs, we observed an improvement inH(latency period, LP) andM(Amax)waves, compared to the group without therapy after SCI. For genetically modified hUCB-MCs, there was improvement inAmaxofMwave and LP of both theMandHwaves. The ratio betweenAmaxof theHandMwaves (Hmax/Mmax) demonstrated that transplantation into the area of SCI of genetically modified hUCB-MCs was more effective than nontransduced hUCB-MCs. Spared tissue and myelinated fibers were increased at day 30 after SCI and transplantation of hUCB-MCs in the lateral and ventral funiculi 2.5 mm from the lesion epicenter. Transplantation of hUCB-MCs genetically modified with the VEGF and GNDF genes significantly increased the number of spared myelinated fibers (22-fold,P>0.01) in the main corticospinal tract compared to the nontransduced ones. HNA+cells with the morphology of phagocytes and microglia-like cells were found as compact clusters or cell bridges within the traumatic cavities that were lined by GFAP+host astrocytes. Our results show that hUCB-MCs transplanted into the site of SCI improved regeneration and that hUCB-MCs genetically modified with the VEGF and GNDF genes were more effective than nontransduced hUCB-MCs.

Use of Human Umbilical Cord Serum to Treat Animal Skin Burns

2019 ◽  
Vol 28 (2) ◽  
pp. 165-173
Author(s):  
Hanan J. Kassab ◽  
Maysoon A. MERDAW ◽  
Intesar J. Al- Ramahi ◽  
Alaa A. Alasadi ◽  
Sawsan Al Mousauy ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Burn Injury ◽  
Healing Process ◽  
Boiling Water ◽  
Control Group ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Hair Regrowth

Objective: The objective of the study was to test the hypothesis, that umbilical cord blood crude serum applied topically with its high concentration of growth factors may promote an early healing for animal models with burn injury. Methods:  Fresh human umbilical cord blood UCBS was collected and screened for transmitted diseases such as hepatitis B, hepatitis C and HIV. Mice and rabbits were scalded by boiling water and chemical burning by NaOH, then treated with UCBS. In comparison with a Cetrimide treated group and a control group (without treatment). The UCBS was subjected to microbial testing to demonstrate the presence or absence of extraneous viable contaminating microorganisms. Results: Topical application of UCBS promoted the healing process; complete healing was seen after 10 days for the mice group and in 7 days in the rabbit group. Cetrimide group applied to the rabbit showed slow healing and needed 10 days for hair regrowth. Control group in both mice and rabbits showed very slow response and the burn area diameter remained the same for over 10 days, and no hair regrowth was obvious after 10 days.    Conclusion: The results of the current study indicated that UCBS is a promising therapy for the healing of burns by boiling water and/or chemical substance, more clinical trials are needed to further explore the long-term effects after UCBS use.

scholarly journals Intrathecal injection of human umbilical cord blood stem cells attenuates spinal cord ischaemic compromise in rats

2014 ◽  
Vol 18 (6) ◽  
pp. 757-762 ◽  
Author(s):  
Gustavo Ieno Judas ◽  
Sueli Gomes Ferreira ◽  
Rafael Simas ◽  
Paulina Sannomiya ◽  
Anderson Benício ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Intrathecal Injection ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

scholarly journals Large- Scale Analysis of MicroRNA Expression in Motor Neuron- like Cells derived from Human Umbilical Cord Blood Mesenchymal Stem Cells

2020 ◽  
Author(s):  
Davood Sanooghi ◽  
Abolfazl Lotfi ◽  
Faezeh Faghihi ◽  
Afzal Karimi ◽  
Zohreh Bagher ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Islet 1

Abstract Background Motor neuron- related disorders such as Spinal Cord Injuries and Amyotrophic Lateral Sclerosis are extremely common around the world. Many efforts have been made to use stem cells to modulate regeneration of spinal cord damages. Human umbilical cord blood mesenchymal stem cells (CB-MSCs) cover a class of cells with self-renewal feature and multilineage differentiation capacity. Retinoic acid(RA) and sonic hedgehog(Shh) are two morphogens responsible in motorneuron commitment during development. This study aims to explore the effect of Shh and RA on differentiation of CB-MSCs into motor neuron- like cells and to determine the related microRNA profile. To do that, human MSCs were isolated and then characterized using flowcytometry. The cells were induced using RA and Shh and the outcomes were assessed by immunocytochemistry, real-time- PCR, and flowcytometry. MicroRNA analysis was performed using Solexa system at three libraries, including Test 1 (with RA and Shh), Test 2 (After removing RA and Shh) and the Control. Results The isolated cells were spindle shape and could express MSC markers confirmed by flowcytometry. The cells could express motorneuron- related markers including Islet-1, Hb-9, SMI-32 and ChAT at the level of mRNA and protein, when treated with RA and Shh. Two weeks after induction, the expression of Neun and Islet-1 declined. The analysis of miRNA sequencing revealed a significant expression of mir-let-7b, mir-137 and mir-324-5p, which were responsible for neuron/motor neuron differentiation and suppression of neural progenitor cell proliferation. Moreover, some novel microRNAs involved in cholinergic, Jak- Stat, Hedgehog and Map kinase signaling pathways were revealed. Conclusion CB-MSC represents a type of cells with convenient accessibility, which can be differentiated into motor neuron- like cells in the presence of RA and Shh. We could also detect the expression of candid microRNAs responsible in motor neuron differentiation and some novel microRNAs involved in cholinergic, Jak- Stat, Hedgehog and Map kinase signaling pathways that must be functionally evaluated in further studies.

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