scholarly journals Identification and Characterization of MicroRNAs inMacaca fascicularisby EST Analysis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Hao Yang ◽  
Haiyang Zhang ◽  
Lin Zhu ◽  
Chenyu Zhang ◽  
Donghai Li
Keyword(s):  
Macaca Fascicularis ◽  
Expressed Sequence Tag ◽  
Est Analysis ◽  
Small Noncoding Rnas ◽  
Novel Mirna ◽  
Medical Experiments ◽  
Alternative Approach ◽  
Model Animal ◽  
Identification And Characterization

MicroRNAs (miRNAs) are small noncoding RNAs which repress gene expression at the posttranscriptional level. In this study, an expressed sequence tag (EST)-based combined method was applied for the detection of miRNAs inMacaca fasciculariswhich is used as a model animal extensively in medical experiments, particularly those involved with neuroscience and disease. Initially, previously known miRNA sequences from metazoans were used to blast with the EST databases ofMacaca fascicularis, and then a range of filtering criteria was conducted to remove some pseudo ones. At last a total of 8 novel conserved miRNAs were identified; their functions were further predicted and analyzed. Together, our study provides insight into miRNAs and their functions inMacaca fascicularis, indicating that the EST analysis is an efficient and affordable alternative approach for identifying novel miRNA candidates.

Identification and characterization of P31m, a novel sperm protein inCynomolgusmonkey (Macaca fascicularis)

2001 ◽  
Vol 59 (4) ◽  
pp. 431-441 ◽  
Author(s):  
Nathalie Lamontagne ◽  
Christine Légaré ◽  
Christian Gaudreault ◽  
Robert Sullivan
Keyword(s):  
Macaca Fascicularis ◽  
Sperm Protein ◽  
Identification And Characterization

scholarly journals Identification and Characterization of CYP2C18 in the Cynomolgus Macaque (Macaca fascicularis)

2010 ◽  
Vol 72 (2) ◽  
pp. 225-228 ◽  
Author(s):  
Yasuhiro UNO ◽  
Kiyomi MATSUNO ◽  
Chika NAKAMURA ◽  
Masahiro UTOH ◽  
Hiroshi YAMAZAKI
Keyword(s):  
Macaca Fascicularis ◽  
Cynomolgus Macaque ◽  
Identification And Characterization

scholarly journals Identification and characterization of new plant microRNAs using EST analysis

Cell Research ◽  
2005 ◽  
Vol 15 (5) ◽  
pp. 336-360 ◽  
Author(s):  
Bao Hong ZHANG ◽  
Xiao Ping PAN ◽  
Qing Lian WANG ◽  
George P COBB ◽  
Todd A ANDERSON
Keyword(s):  
Est Analysis ◽  
Identification And Characterization

scholarly journals Identification and Characterization of Small Noncoding RNAs in Genome Sequences of the Edible FungusPleurotus ostreatus

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Jibin Qu ◽  
Mengran Zhao ◽  
Tom Hsiang ◽  
Xiaoxing Feng ◽  
Jinxia Zhang ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Noncoding Rnas ◽  
Genome Sequences ◽  
Small Noncoding Rnas ◽  
Basidiomycetous Fungus ◽  
A Genome ◽  
Genome Assemblies ◽  
Genome Scale ◽  
Identification And Characterization

Noncoding RNAs (ncRNAs) have been identified in many fungi. However, no genome-scale identification of ncRNAs has been inventoried for basidiomycetes. In this research, we detected 254 small noncoding RNAs (sncRNAs) in a genome assembly of an isolate (CCEF00389) ofPleurotus ostreatus, which is a widely cultivated edible basidiomycetous fungus worldwide. The identified sncRNAs include snRNAs, snoRNAs, tRNAs, and miRNAs. SnRNA U1 was not found in CCEF00389 genome assembly and some other basidiomycetous genomes by BLASTn. This implies that if snRNA U1 of basidiomycetes exists, it has a sequence that varies significantly from other organisms. By analyzing the distribution of sncRNA loci, we found that snRNAs and most tRNAs (88.6%) were located in pseudo-UTR regions, while miRNAs are commonly found in introns. To analyze the evolutionary conservation of the sncRNAs inP. ostreatus, we aligned all 254 sncRNAs to the genome assemblies of some other Agaricomycotina fungi. The results suggest that most sncRNAs (77.56%) were highly conserved inP. ostreatus, and 20% were conserved in Agaricomycotina fungi. These findings indicate that most sncRNAs ofP. ostreatuswere not conserved across Agaricomycotina fungi.

scholarly journals Identification and characterization of GSTT3, a third murine Theta class glutathione transferase

2002 ◽  
Vol 366 (1) ◽  
pp. 323-332 ◽  
Author(s):  
Marjorie COGGAN ◽  
Jack U. FLANAGAN ◽  
Michael W. PARKER ◽  
Vanicha VICHAI ◽  
William R. PEARSON ◽  
...  
Keyword(s):  
Glutathione Transferase ◽  
Expressed Sequence Tag ◽  
Molecular Model ◽  
Binding Pocket ◽  
Gene Encoding ◽  
Hydrophobic Substrate ◽  
Intron Structure ◽  
Identification And Characterization ◽  
Expressed Sequence

A novel Theta class glutathione transferase (GST) isoenzyme from mouse termed mGSTT3 has been identified by analysis of the expressed sequence tag database. The gene encoding mGSTT3 is clustered with the mGSTT1 and mGSTT2 genes on chromosome 10 and has an exon/intron structure that is similar to that of the other Theta class genes. mGSTT3 is expressed strongly in the liver and to a decreasing extent in the kidney and testis. Recombinant mGSTT3-3 expressed in Escherichia coli had a substrate-specificity profile that differed significantly from that of GSTT1-1 and GSTT2-2 isoenzymes. A molecular model of mGSTT3 suggested that, in comparison with GSTT2, a decrease in volume of the hydrophobic substrate-binding site and the loss of the sulphate-binding pocket prevents its use of the GSTT2 substrate 1-menaphthyl sulphate.

scholarly journals Expressed Sequence Tag Analysis for Identification and Characterization of Sex-Related Genes in the Giant Tiger Shrimp Penaeus monodon

BMB Reports ◽  
2007 ◽  
Vol 40 (4) ◽  
pp. 501-510 ◽  
Author(s):  
Rachanimuk Preechaphol ◽  
Rungnapa Leelatanawit ◽  
Kanchana Sittikankeaw ◽  
Sirawut Klinbunga ◽  
Bavornlak Khamnamtong ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Penaeus Monodon ◽  
Tiger Shrimp ◽  
Expressed Sequence Tag Analysis ◽  
Identification And Characterization ◽  
Expressed Sequence
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