Monocyte Locomotion Inhibitory Factor Produced byE. histolyticaImproves Motor Recovery and Develops Neuroprotection after Traumatic Injury to the Spinal Cord

Monocyte locomotion inhibitory factor (MLIF) is a pentapeptide produced byEntamoeba histolyticathat has a potent anti-inflammatory effect. Either MLIF or phosphate buffered saline (PBS) was administered directly onto the spinal cord (SC) immediately after injury. Motor recovery was evaluated. We also analyzed neuroprotection by quantifying the number of surviving ventral horn motor neurons and the persistence of rubrospinal tract neurons. To evaluate the mechanism through which MLIF improved the outcome of SC injury, we quantified the expression of inducible nitric oxide synthase (iNOS), interleukin-10 (IL-10), and transforming growth factor-β(TGF-β) genes at the site of injury. Finally, the levels of nitric oxide and of lipid peroxidation were also determined in peripheral blood. Results showed that MLIF improved the rate of motor recovery and this correlated with an increased survival of ventral horn and rubrospinal neurons. These beneficial effects were in turn associated with a reduction in iNOS gene products and a significant upregulation of IL-10 and TGF-βexpression. In the same way, MLIF reduced the concentration of nitric oxide and the levels of lipid peroxidation in systemic circulation. The present results demonstrate for the first time the neuroprotective effects endowed by MLIF after SC injury.

Download Full-text

Lewis, Fischer 344, and Sprague-Dawley Rats Display Differences in Lipid Peroxidation, Motor Recovery, and Rubrospinal Tract Preservation after Spinal Cord Injury

Frontiers in Neurology ◽

10.3389/fneur.2015.00108 ◽

2015 ◽

Vol 6 ◽

Cited By ~ 9

Author(s):

Humberto Mestre ◽

Manuel Ramirez ◽

Elisa Garcia ◽

Susana MartiÃ±Ã³n ◽

Yolanda Cruz ◽

...

Keyword(s):

Spinal Cord ◽

Lipid Peroxidation ◽

Spinal Cord Injury ◽

Motor Recovery ◽

Sprague Dawley ◽

Rubrospinal Tract ◽

Fischer 344 ◽

Sprague Dawley Rats ◽

Cord Injury

Download Full-text

Neuroprotective effects of a potent bradykinin B2 receptor antagonist HOE-140 on microvascular permeability, blood flow disturbances, edema formation, cell injury and nitric oxide synthase upregulation following trauma to the spinal cord

International Review of Neurobiology - New Therapeutic Strategies for Brain Edema and Cell Injury ◽

10.1016/bs.irn.2019.06.008 ◽

2019 ◽

pp. 103-152 ◽

Cited By ~ 3

Author(s):

Hari Shanker Sharma ◽

Lianyuan Feng ◽

Dafin Fior Muresanu ◽

Rudy J. Castellani ◽

Aruna Sharma

Keyword(s):

Nitric Oxide ◽

Spinal Cord ◽

Blood Flow ◽

Nitric Oxide Synthase ◽

Cell Injury ◽

Neuroprotective Effects ◽

Edema Formation ◽

Flow Disturbances ◽

Hoe 140 ◽

Oxide Synthase

Download Full-text

Effect of transforming growth factor beta 1 (TGF-beta 1) on nitric oxide production and lipid peroxidation in oral mucosal wound healing

Medicinal Chemistry Research ◽

10.1007/s00044-009-9276-7 ◽

2009 ◽

Vol 20 (1) ◽

pp. 23-28 ◽

Cited By ~ 9

Author(s):

Şule Coşkun ◽

Emine Gülçeri Güleç Peker ◽

Barbaros Balabanlı ◽

Seyhan Ahıska ◽

Füsun Acartürk

Keyword(s):

Nitric Oxide ◽

Lipid Peroxidation ◽

Wound Healing ◽

Growth Factor ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Nitric Oxide Production ◽

Oxide Production ◽

Growth Factor Beta ◽

Mucosal Wound

Download Full-text

Neuroprotection by Paeoniflorin against Nuclear Factor Kappa B-Induced Neuroinflammation on Spinal Cord Injury

BioMed Research International ◽

10.1155/2018/9865403 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Bin Wang ◽

Wangying Dai ◽

Lijun Shi ◽

Honglin Teng ◽

Xigong Li ◽

...

Keyword(s):

Nitric Oxide ◽

Spinal Cord ◽

Spinal Cord Injury ◽

Motor Function ◽

Nuclear Factor Kappa B ◽

Nuclear Factor ◽

Neuroprotective Effects ◽

Regulatory Pathways ◽

Nuclear Factor Kappa ◽

Cord Injury

Background. Acute spinal cord injury (SCI) is one of the most common and devastating causes of sensory or motor dysfunction. Nuclear factor-kappa B(NF-κB)-mediated neuroinflammatory responses, in addition to nitric oxide (NO), are key regulatory pathways in SCI. Paeoniflorin (PF), a major active component extracted from Paeonia roots, has been suggested to exert neuroprotective effects in the central nervous system. However, whether PF could improve the motor function after SCI in vivo is still unclear. Method. Immunohistochemical analysis, western blot, real-time quantitative PCR, immunofluorescence staining, and histopathological and behavioral evaluation were used to explore the effects of paeoniflorin after SCI for 14 days. Results. In this study, PF treatment significantly inhibited NF-κB activation and downregulated the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2), and Nogo-A. Comparing behavioral and histological changes in SCI and PF treatment groups, we found that PF treatment improved motor function recovery, attenuated the histopathological damage, and increased neuronal survival in the SCI model. PF treatment also reduced expression levels of Bax and c-caspase-3 and increased the expression level of Bcl-2 and cell viabilities. Upregulation of TNF-α, IL-6, and IL-1β after injury was also prevented by PF. Conclusion. These results suggest that the neuroprotective effects of PF are related to the inhibition of the NF-κB signaling pathway. And PF may be a therapeutic strategy in spinal cord injury.

Download Full-text