Bovine Papillomavirus Type 2 Infection and Microscopic Patterns of Urothelial Tumors of the Urinary Bladder in Water Buffaloes

Microscopic patterns of thirty-four urothelial tumors of the urinary bladder of water buffaloes from the Marmara and Black Sea Regions of Turkey are here described. All the animals grazed on lands rich in bracken fern. Histological diagnosis was assessed using morphological parameters recently suggested for the urinary bladder tumors of cattle. Papillary carcinoma was the most common neoplastic lesion (22/34) observed in this study, and low-grade carcinoma was more common (seventeen cases) than high-grade carcinoma (five cases). Papilloma, papillary urothelial neoplasm of low malignant potential (PUNLMP), and invasive carcinomas were less frequently seen. Carcinoma in situ (CIS) was often detected associated with some papillary and invasive carcinomas. De novo (primary) CIS was rare representing 3% of tumors of this series. A peculiar feature of the most urothelial tumors was the presence in the tumor stroma of immune cells anatomically organized in tertiary lymphoid organs (TLOs). Bovine papillomavirus type-2 (PV-2) E5 oncoprotein was detected by molecular and immunohistochemistry procedures. Early protein, E2, and late protein, L1, were also detected by immunohistochemical studies. Morphological and molecular findings show that BPV-2 infection contributes to the development of urothelial bladder carcinogenesis also in water buffaloes.

Download Full-text

Bovine Papillomavirus Type-2 (BPV-2) Infection and Expression of Uroplakin IIIb, a Novel Urothelial Cell Marker, in Urinary Bladder Tumors of Cows

Veterinary Pathology ◽

10.1354/vp.42-6-812 ◽

2005 ◽

Vol 42 (6) ◽

pp. 812-818 ◽

Cited By ~ 19

Author(s):

S. Roperto ◽

V. Ambrosio ◽

G. Borzacchiello ◽

P. Galati ◽

O. Paciello ◽

...

Keyword(s):

Urinary Bladder ◽

Single Cells ◽

Urothelial Cell ◽

Biological Behavior ◽

Bovine Papillomavirus ◽

Immunohistochemical Markers ◽

Cell Layers ◽

Urothelial Tumors ◽

Differentiation Pattern

Several immunohistochemical markers have been used to define the differentiation pattern of urothelial cell tumors of the urinary bladder. We investigated the expression of the recently characterized uroplakin (UP) IIIb, an urothelium-specific and differentiation-dependent protein, in 39 urothelial tumors of the urinary bladder in cows that had suffered from chronic enzootic hematuria for several years. Bovine papillomavirus type 2 DNA was amplified and UP IIIb protein was detected in all these tumors. In papillomas and papillary carcinomas, UP IIIb expression was mostly seen as superficial staining; luminal and peripheral patterns were also observed. In nonpapillary carcinomas, UP IIIb appeared to define clearly the cell membrane lining intercellular and intracellular lumina as well as the cell borders in deeper cell layers. In benign and malignant lesions, an intracytoplasmic immunoreactivity was also detected. Coarse intracytoplasmic UP IIIb-positive material close to the nucleus occurred in some malignant cells. Focally strong membraneous immunostaining that marked single cells with complete ringlike peripheral pattern was seen. Although UP IIIb expression does not seem to correlate with the biological behavior of urothelial tumors, it appears to be a highly sensitive marker for bovine urothelial tumors.

Download Full-text

Productive Infection of Bovine Papillomavirus Type 2 in the Urothelial Cells of Naturally Occurring Urinary Bladder Tumors in Cattle and Water Buffaloes

PLoS ONE ◽

10.1371/journal.pone.0062227 ◽

2013 ◽

Vol 8 (5) ◽

pp. e62227 ◽

Cited By ~ 22

Author(s):

Sante Roperto ◽

Valeria Russo ◽

Ayhan Ozkul ◽

Annunziata Corteggio ◽

Aylin Sepici-Dincel ◽

...

Keyword(s):

Urinary Bladder ◽

Productive Infection ◽

Bovine Papillomavirus ◽

Urothelial Cells ◽

Bladder Tumors ◽

Water Buffaloes ◽

Naturally Occurring

Download Full-text

Bovine Papillomavirus Type 2 (BPV-2) E5 Oncoprotein Binds to the Subunit D of the V1-ATPase Proton Pump in Naturally Occurring Urothelial Tumors of the Urinary Bladder of Cattle

PLoS ONE ◽

10.1371/journal.pone.0088860 ◽

2014 ◽

Vol 9 (2) ◽

pp. e88860 ◽

Cited By ~ 5

Author(s):

Sante Roperto ◽

Valeria Russo ◽

Giuseppe Borzacchiello ◽

Chiara Urraro ◽

Roberta Lucà ◽

...

Keyword(s):

Urinary Bladder ◽

Proton Pump ◽

Bovine Papillomavirus ◽

Naturally Occurring ◽

V1 Atpase ◽

E5 Oncoprotein ◽

Urothelial Tumors

Download Full-text

Urothelial Tumors of the Urinary Bladder in Young Patients: A Clinicopathologic Study of 59 Cases

Archives of Pathology & Laboratory Medicine ◽

10.5858/arpa.2012-0322-oa ◽

2013 ◽

Vol 137 (10) ◽

pp. 1337-1341 ◽

Cited By ~ 23

Author(s):

Melissa L. Stanton ◽

Li Xiao ◽

Bogdan A. Czerniak ◽

Charles C. Guo

Keyword(s):

Urinary Bladder ◽

Clinical Outcomes ◽

Young Patients ◽

Muscularis Propria ◽

Low Grade ◽

High Grade ◽

Urothelial Carcinomas ◽

Pathologic Features ◽

Urothelial Tumors

Context.—Urothelial tumors are rare in young patients. Because of their rarity, the natural history of the disease in young patients remains poorly understood. Objective.—To understand the pathologic and clinical features of urothelial tumors of the urinary bladder in young patients. Design.—We identified 59 young patients with urothelial tumors of the urinary bladder treated at our institution and analyzed the tumors' pathologic features and the patients' clinical outcomes. Results.—All patients were 30 years or younger, with a mean age of 23.5 years (range, 4–30). Thirty-eight patients (64%) were male, and 21 (36%) were female. Most tumors were noninvasive, papillary urothelial tumors (49 of 59; 83%), including papillary urothelial neoplasms of low malignant potential (7 of 49; 14%), low-grade papillary urothelial carcinomas (38 of 49; 78%), and high-grade papillary urothelial carcinomas (4 of 49; 8%). Only a few (n = 10) of the urothelial tumors were invasive, invading the lamina propria (n = 5; 50%), muscularis propria (n = 4; 40%), or perivesical soft tissue (n = 1; 10%). Clinical follow-up information was available for 41 patients (69%), with a mean follow-up time of 77 months. Of 31 patients with noninvasive papillary urothelial tumors, only 1 patient (3%) later developed an invasive urothelial carcinoma and died of the disease, and 30 of these patients (97%) were alive at the end of follow-up, although 10 (32%) had local tumor recurrences. In the 10 patients with invasive urothelial carcinomas, 3 patients (30%) died of the disease and 5 others (50%) were alive with metastases (the other 2 [20%] were alive with no recurrence). Conclusion.—Urothelial tumors in young patients are mostly noninvasive, papillary carcinomas and have an excellent prognosis; however, a small subset of patients may present with high-grade invasive urothelial carcinomas that result in poor clinical outcomes.

Download Full-text

Polyps and Masses of the Pediatric Urinary Bladder: A 21-Year Pathology Review

Pediatric and Developmental Pathology ◽

10.2350/11-01-0958-oa.1 ◽

2011 ◽

Vol 14 (6) ◽

pp. 438-444 ◽

Cited By ~ 22

Author(s):

Alison R. Huppmann ◽

Bruce R. Pawel

Keyword(s):

Urinary Bladder ◽

Pediatric Population ◽

Low Grade ◽

Nephrogenic Adenoma ◽

Patient Demographics ◽

Urothelial Tumors ◽

Histologic Types ◽

Cystitis Cystica ◽

Pathology Review ◽

Made In

Although not uncommon in adults, bladder tumors are rare in children. In addition, the histologic types of tumors seen in the pediatric population differ from those seen in adults. Although rhabdomyosarcoma is the most common pediatric bladder tumor, many other benign, malignant, and reactive lesions can be encountered. All may present clinically as a mass or polyp in the bladder. This study was designed to describe the pathology and patient demographics of pediatric bladder masses, because there are few studies describing these entities. Retrospectively reviewing our experience over a 21-year period, we identified 98 specimens from 65 patients with polyps or masses in the urinary bladder. As expected, the most frequent diagnosis was rhabdomyosarcoma. This was followed by fibroepithelial polyp and a variety of additional nonurothelial tumors. Only 7 urothelial tumors were identified, including 1 low-grade papillary urothelial carcinoma. Inflammatory lesions, such as cystitis cystica and nephrogenic adenoma, were invariably associated with an irritating factor when a history was provided. Our findings emphasize that diagnoses made in the pediatric urinary bladder are distinct from those in adults, although a wide variety of lesions may still be seen.

Download Full-text

Bovine papillomavirus type 2 infects the urinary bladder of water buffalo (Bubalus bubalis) and plays a crucial role in bubaline urothelial carcinogenesis

Journal of General Virology ◽

10.1099/vir.0.047662-0 ◽

2013 ◽

Vol 94 (2) ◽

pp. 403-408 ◽

Cited By ~ 34

Author(s):

Sante Roperto ◽

Valeria Russo ◽

Ayhan Ozkul ◽

Aylin Sepici-Dincel ◽

Paola Maiolino ◽

...

Keyword(s):

Urinary Bladder ◽

Cell Transformation ◽

Bubalus Bubalis ◽

Immunofluorescence Assay ◽

Receptor Interaction ◽

Bovine Papillomavirus ◽

Clinically Normal ◽

Bladder Carcinogenesis ◽

Β Receptor

Bovine papillomavirus type 2 (BPV-2) has been shown to infect and play a role in urinary bladder carcinogenesis of buffaloes grazed on pastures with ferns from the Marmara and Black Sea Regions of Turkey. BPV-2 DNA has been found in both neoplastic and non-neoplastic lesions of the urinary bladder. Furthermore, this virus may be a normal inhabitant of the urinary bladder since BPV-2 DNA has also been detected in clinically normal buffaloes. The viral activation by fern immunosuppressant or carcinogen may trigger the urothelial cell transformation. The E5 oncoprotein was solely detected in urothelial tumours and appeared to be co-localized with the overexpressed and phosphorylated platelet derived growth factor (PDGF) β receptor in a double-colour immunofluorescence assay. Our results indicate that the E5–PDGF β receptor interaction also occurs in spontaneous tumours of the bubaline urinary bladder, revealing an additional role of BPV-2 in bladder carcinogenesis of buffaloes.

Download Full-text

Spectrum of Lesions in Urinary Bladder- A Histopathological Study

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v6i2.22473 ◽

2018 ◽

Vol 6 (2) ◽

pp. 24-27 ◽

Cited By ~ 1

Author(s):

Anita Shah ◽

Manglesh Srivastava ◽

Ashok Samdurkar ◽

Ghanshyam Sigdel

Keyword(s):

Urinary Bladder ◽

Early Stage ◽

Granulomatous Inflammation ◽

Urinary Bladder Cancer ◽

Histopathological Study ◽

Common Source ◽

Institutional Review ◽

Neoplastic Lesion ◽

Neoplastic Lesions ◽

Urothelial Tumors

Introduction: The lesions of urinary bladder both non-neoplastic and neoplastic pose a common source of both morbidity and mortality. An accurate diagnosis of these lesions requires cystoscopy which allows a direct visualization of the bladder mucosa and biopsies of suspected lesions. Urinary bladder cancer is sixth most common cancer worldwide and represents a heterogeneous group of neoplasms. The current study aimed to study the different bladder lesions and its clinical features to detect it in early stage and as a mainstay option in the diagnosis and follow up. Materials and methods: This was a retrospective analysis of biopsies of urinary bladder submitted to the department of pathology over a period of 12 months. The study was approved by the institutional review board of the Universal College of Medical Sciences (UCMS-TH). All the urinary bladder biopsies received in the department were included in the study whereas autolysis of specimen and inadequate biopsies were excluded. Results: Among the 36 cases of urinary bladder lesions, the majority (35.36%) were in age group 61-70 years (22.33%). The patients had combination of lower urinary tract symptoms, the commonest being hematuria. 30.55% had non-neoplastic lesions and 69.55% had neoplastic lesion. Among non- neoplastic cases, 5.55% had chronic granulomatous inflammation. Most common neoplastic lesions was infiltrating urothelial carcinoma (n=6) followed by non- invasive urothelial neoplasia (n=5). Conclusion: A variety of lesions occur in urinary bladder and is commonly encountered by pathologist. Hematuria was commonest symptom and the clinicians investigated these patients further, which led to discovery of the urothelial tumors. Identification of these patients has an important impact on prognosis as well as on therapeutic approach.

Download Full-text

Utility of Exfoliative Cytology of Voided Urine in Comparison with Ultrasonogram and Cystoscopy Findings in the Diagnosis of Urothelial Cancers

TAJ Journal of Teachers Association ◽

10.3329/taj.v30i2.39136 ◽

2018 ◽

Vol 30 (2) ◽

pp. 39-46

Author(s):

SM Badruddoza ◽

FA Azim ◽

AJE Nahar Rahman ◽

M Kamal ◽

AR Barua ◽

...

Keyword(s):

Urinary Bladder ◽

Cell Carcinoma ◽

Urine Cytology ◽

Invasive Technique ◽

Published Data ◽

Low Grade ◽

Invasive Squamous Cell Carcinoma ◽

Invasive Carcinomas ◽

Positive Results ◽

Grade Iii

Carcinoma of the urinary bladder affects men more often than women and occurs in patients over the age of 50 years. Transitional cell carcinoma (TCC) comprises about 90% of all primary tumor of urinary bladder. In this study a total of 57 cases were taken. The aim of the study was to establish diagnostic role of voided urine cytology, ultrasonography and cystoscopy in urothelial cancers. Out of 57 cases, cytological diagnosis was positive in 53 cases (93%) and negative in 4 cases (7%). There were no atypical or suspicious cases. Of the total cases, cytology was negative only in 7% cases. Among 42 invasive carcinomas 14 (33%) grade III invasive flat carcinoma, one (2%) invasive papillary adenocarcinoma and two (5%) invasive squamous cell carcinoma, each of them gave 100% positive cytology. Of the remaining 25 (60%) invasive papillary TCC, 21 were in grade II and 4 were in grade III. They gave 90% and 100% positive cytologic results. Ultrasonogram of urinary bladder gave 95% (approx.) positive results and out of 57 cases, 3 cases failed to give positive results in ultrasonogram (USG) of the urinary tract. Cystoscopy detected tumor mass in 98% cases and it gave false negative result in 2% cases. Fifty-two tumors had a diameter ranging 1.1- 6 cm. Single lesions were seen in 40 cases, double lesion in one case and multiple in 13 cases. The probability of later invasive carcinomas nearly tripled in-patients with multiple lesions on presentation (13.6%) compared with those who presented with a single lesion (4.6%). Consistent with previously published data, this study showed the highest diagnostic accuracy with high-grade tumors and lowest with low-grade tumors. So in a proper clinical setting, non-invasive technique like voided urine cytology and ultrasonography could be done as a first line cost effective method in the diagnosis of urothelial cancers.TAJ 2017; 30(2): 39-46

Download Full-text