scholarly journals Purification and Characterization of a Unique Pectin Lyase from Aspergillus giganteus Able to Release Unsaturated Monogalacturonate during Pectin Degradation

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Danielle Biscaro Pedrolli ◽  
Eleonora Cano Carmona
Keyword(s):  
Divalent Cations ◽  
Orange Peel ◽  
Pectin Lyase ◽  
Soluble Product ◽  
Aspergillus Giganteus ◽  
Pectic Substances ◽  
Orange Peel Waste ◽  
Pectin Lyases ◽  
Vegetable Oil Extraction

A pectin lyase, named PLIII, was purified to homogeneity from the culture filtrate of Aspergillus giganteus grown in submerged culture containing orange peel waste as carbon source. PLIII was able to digest apple pectin and citrus pectins with different degrees of methyl esterification. Interestingly, the PLIII activity was stimulated in the presence of some divalent cations including Pb2+ and was not significantly affected by Hg2+. Like other pectin lyases, PLIII is stimulated by but is not dependent on Ca2+. The main soluble product released during the degradation of pectic substances promoted by the PLIII is compatible with an unsaturated monogalacturonate. PLIII is a unique enzyme able to release unsaturated monogalacturonate as the only soluble product during the degradation of pectic substances; therefore, PLIII was classified as an exo-pectin lyase. To our knowledge, this is the first characterization of an exo-pectin lyase. The PLIII described in this work is potentially useful for ethanol production from pectin-rich biomass, besides other common applications for alkaline pectinases like preparation of textile fibers, coffee and tea fermentation, vegetable oil extraction, and the treatment of pulp in papermaking.

scholarly journals Characterization of purified <i>β</i>-glucosidase produced from <i>Trichoderma viride</i> through bio-processing of orange peel waste

2013 ◽  
Vol 04 (10) ◽  
pp. 941-944 ◽  
Author(s):  
Muhammad Irshad ◽  
Zahid Anwar ◽  
Muhammad Ramzan ◽  
Zahed Mahmood ◽  
Haq Nawaz
Keyword(s):  
Trichoderma Viride ◽  
Orange Peel ◽  
Orange Peel Waste

scholarly journals Characterization of Orange Peel Waste and Valorization to Obtain Reducing Sugars

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1348
Author(s):  
José R. Ayala ◽  
Gisela Montero ◽  
Marcos A. Coronado ◽  
Conrado García ◽  
Mario A. Curiel-Alvarez ◽  
...  
Keyword(s):  
Acid Concentration ◽  
Reducing Sugars ◽  
Maximum Yield ◽  
Orange Peel ◽  
World Population ◽  
Hydrolysis Time ◽  
Experimental Conditions ◽  
Hydrolysis Process ◽  
Orange Peel Waste

Annually, millions of tons of foods are generated with the purpose to feed the growing world population. One particular eatable is orange, the production of which in 2018 was 75.54 Mt. One way to valorize the orange residue is to produce bioethanol by fermenting the reducing sugars generated from orange peel. Hence, the objective of the present work was to determine the experimental conditions to obtain the maximum yield of reducing sugars from orange peel using a diluted acid hydrolysis process. A proximate and chemical analysis of the orange peel were conducted. For the hydrolysis, two factorial designs were prepared to measure the glucose and fructose concentration with the 3,5-DNS acid method and UV-Visible spectroscopy. The factors were acid concentration, temperature and hydrolysis time. After the hydrolysis, the orange peel samples were subjected to an elemental SEM-EDS analysis. The results for the orange peel were 73.530% of moisture, 99.261% of volatiles, 0.052% of ash, 0.687% of fixed carbon, 19.801% of lignin, 69.096% of cellulose and 9.015% of hemicellulose. The highest concentration of glucose and fructose were 24.585 and 9.709 g/L, respectively. The results highlight that sugar production is increased by decreasing the acid concentration.

Preparation using Pectinase and Characterization of Nanofibers from Orange Peel Waste in Juice Factories

2014 ◽  
Vol 79 (6) ◽  
pp. N1218-N1224 ◽  
Author(s):  
Akihiro Hideno ◽  
Kentaro Abe ◽  
Hiroyuki Yano
Keyword(s):  
Orange Peel ◽  
Orange Peel Waste

scholarly journals Characterization of a Cytotoxic Factor in Culture Filtrates of Serratia marcescens

2002 ◽  
Vol 70 (3) ◽  
pp. 1121-1128 ◽  
Author(s):  
Kent B. Marty ◽  
Christopher L. Williams ◽  
Linda J. Guynn ◽  
Michael J. Benedik ◽  
Steven R. Blanke
Keyword(s):  
Cytotoxic Activity ◽  
Serratia Marcescens ◽  
Mammalian Cells ◽  
Divalent Cations ◽  
Recombinant Expression ◽  
Cytotoxic Factor ◽  
Culture Filtrates ◽  
Type Strains

ABSTRACT Serratia marcescens culture filtrates have been reported to be cytotoxic to mammalian cells. Using biochemical and genetic approaches, we have identified a major source of this cytotoxic activity. Both heat and protease treatments abrogated the cytotoxicity of S. marcescens culture filtrates towards HeLa cells, suggesting the involvement of one or more protein factors. A screen for in vitro cytotoxic activity revealed that S. marcescens mutant strains that are deficient in production of a 56-kDa metalloprotease are significantly less cytotoxic to mammalian cells. Cytotoxicity was significantly reduced when culture filtrates prepared from wild-type strains were pretreated with either EDTA or 1,10-phenanthroline, which are potent inhibitors of the 56-kDa metalloprotease. Furthermore, cytotoxic activity was restored when the same culture filtrates were incubated with zinc divalent cations, which are essential for enzymatic activity of the 56-kDa metalloprotease. Finally, recombinant expression of the S. marcescens 56-kDa metalloprotease conferred a cytotoxic phenotype on the culture filtrates of a nonpathogenic Escherichia coli strain. Collectively, these data suggest that the 56-kDa metalloprotease contributes significantly to the in vitro cytotoxic activity commonly observed in S. marcescens culture filtrates.

BIOPLASTIC MADE OF ORANGE PEELS

10.6036/10212 ◽  
2021 ◽  
Vol DYNA-ACELERADO (0) ◽  
pp. [ 7 pp]-[ 7 pp]
Author(s):  
Julieta Domínguez Soberanes ◽  
PIA BERGER
Keyword(s):  
Organic Waste ◽  
Raw Materials ◽  
Orange Peel ◽  
Main Ingredient ◽  
Resistant Material ◽  
Orange Peels ◽  
Toxic Product ◽  
Orange Peel Waste ◽  
Materials Used ◽  
Environmentally Friendly Process

This study uses orange peel waste to create a biopolymer that can be used for different purposes. In order to achieve this, we evaluated various technologies for the production of the biopolymer, and tried to design the most environmentally friendly process possible. One of the reasons why this bioplastic should be manufactured is to participate in the replacement of common environmental hazardous plastic, which has been banned in many places. On the other hand, using orange peel as the main ingredient is an alternative and gives value to an organic waste that has limited use in circular economy solutions. In this research we present a methodology to create a bioplastic of orange peels. As a result, we obtained a biodegradable, flexible and resistant material to be used in the manufacture of containers, utensils, etc. In addition, it is a material that, given the raw materials used, is considered GRAS (Generally Recognized As Save), implying a non-toxic product that is safe for the consumer.

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