Diversity of the DNA Replication System in theArchaeaDomain

The precise and timely duplication of the genome is essential for cellular life. It is achieved by DNA replication, a complex process that is conserved among the three domains of life. Even though the cellular structure of archaea closely resembles that of bacteria, the information processing machinery of archaea is evolutionarily more closely related to the eukaryotic system, especially for the proteins involved in the DNA replication process. While the general DNA replication mechanism is conserved among the different domains of life, modifications in functionality and in some of the specialized replication proteins are observed. Indeed,Archaeapossess specific features unique to this domain. Moreover, even though the general pattern of the replicative system is the same in all archaea, a great deal of variation exists between specific groups.

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[41] Identification of eukaryotic DNA replication proteins using simian virus 40 in Vitro replication system

Methods in Enzymology - DNA Replication ◽

10.1016/0076-6879(95)62043-5 ◽

1995 ◽

pp. 522-548 ◽

Cited By ~ 32

Author(s):

George S. Brush ◽

Thomas J. Kelly ◽

Bruce Stillman

Keyword(s):

Dna Replication ◽

Simian Virus 40 ◽

Simian Virus ◽

Replication Proteins ◽

Replication System ◽

In Vitro Replication ◽

Eukaryotic Dna

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Mechanical Properties of DNA Replication

10.1101/699009 ◽

2019 ◽

Author(s):

Stuart A. Sevier

Keyword(s):

Mechanical Properties ◽

Dna Replication ◽

Helical Structure ◽

Physical Basis ◽

Replication Process ◽

Idealized Model ◽

Cellular Life ◽

Gene Orientation ◽

Mechanical Elements

Central to the function of cellular life is the reading, storage and replication of DNA. Due to the helical structure of DNA, a complicated topological braiding of new strands follows the duplication of the old strands. Even though this was discovered over 60 years ago, the mathematical and physical questions this presents have largely gone unaddressed. In this letter we construct a simple idealized model of DNA replication using only the most basic mathematical and mechanical elements of DNA replication. The aim of this is to reveal the mechanical balance of braided, replicated DNA against the twist of unreplicated DNA at the heart of the replication process. The addition of topoisomerase action is included presenting a balancing force offering a glimpse into the ways in which cells maintain this balance. Additionally the physical basis for recently observed replication/replication and replication/transcription conflicts are examined showing how gene orientation and size can impact DNA replication.

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Development of an in vitro bacteriophage N4 DNA replication system.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)67413-7 ◽

1986 ◽

Vol 261 (23) ◽

pp. 10506-10510

Author(s):

J K Rist ◽

M Pearle ◽

A Sugino ◽

L B Rothman-Denes

Keyword(s):

Dna Replication ◽

Replication System ◽

Bacteriophage N4

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Overlapping Roles of the Spindle Assembly and DNA Damage Checkpoints in the Cell-Cycle Response to Altered Chromosomes in Saccharomyces cerevisiae

Genetics ◽

10.1093/genetics/161.2.521 ◽

2002 ◽

Vol 161 (2) ◽

pp. 521-534

Author(s):

Peter M Garber ◽

Jasper Rine

Keyword(s):

Dna Damage ◽

Dna Replication ◽

Spindle Checkpoint ◽

Dna Lesions ◽

Replication Proteins ◽

Replication Checkpoint ◽

Dna Damage Checkpoints ◽

Dna Replication Checkpoint ◽

Mcm Proteins ◽

Stalled Replication Forks

Abstract The MAD2-dependent spindle checkpoint blocks anaphase until all chromosomes have achieved successful bipolar attachment to the mitotic spindle. The DNA damage and DNA replication checkpoints block anaphase in response to DNA lesions that may include single-stranded DNA and stalled replication forks. Many of the same conditions that activate the DNA damage and DNA replication checkpoints also activated the spindle checkpoint. The mad2Δ mutation partially relieved the arrest responses of cells to mutations affecting the replication proteins Mcm3p and Pol1p. Thus a previously unrecognized aspect of spindle checkpoint function may be to protect cells from defects in DNA replication. Furthermore, in cells lacking either the DNA damage or the DNA replication checkpoints, the spindle checkpoint contributed to the arrest responses of cells to the DNA-damaging agent methyl methanesulfonate, the replication inhibitor hydroxyurea, and mutations affecting Mcm2p and Orc2p. Thus the spindle checkpoint was sensitive to a wider range of chromosomal perturbations than previously recognized. Finally, the DNA replication checkpoint did not contribute to the arrests of cells in response to mutations affecting ORC, Mcm proteins, or DNA polymerase δ. Thus the specificity of this checkpoint may be more limited than previously recognized.

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