scholarly journals Yiqihuoxuejiedu Formula Inhibits Vascular Remodeling by Reducing Proliferation and Secretion of Adventitial Fibroblast after Balloon Injury

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ming-Jing Zhao ◽  
Ai-Ming Wu ◽  
Jie Wang ◽  
Hong Chang ◽  
Yong-Hong Gao ◽  
...  

Vascular remodeling occurs in atherosclerosis, hypertension, and restenosis after percutaneous coronary intervention. Adventitial remodeling may be a potential therapeutic target. Yiqihuoxuejiedu formula uses therapeutic principles from Chinese medicine to supplement Qi, activate blood circulation, and resolve toxin and it has been shown to inhibit vascular stenosis. To investigate effects and mechanisms of the formula on inhibiting vascular remodeling, especially adventitial remodeling, rats with a balloon injury to their common carotid artery were used and were treated for 7 or 28 days after injury. The adventitial area andα-SMA expression increased at 7 days after injury, which indicated activation and proliferation of adventitial fibroblasts. Yiqihuoxuejiedu formula reduced the adventitial areas at 7 days, attenuated the neointima and vessel wall area, stenosis percent, andα-SMA expression in the neointima, and reduced collagen content and type I/III collagen ratio in the adventitia at 28 days. Yiqihuoxuejiedu formula had more positive effects than Captopril in reducing intimal proliferation and diminishing stenosis, although Captopril lowered neointimalα-SMA expression and reduced the collagen content at 28 days. Yiqihuoxuejiedu formula has inhibitory effects on positive and negative remodeling by reducing adventitial and neointimal proliferation, reducing content, and elevating adventitial compliance.

1987 ◽  
Vol 117 (4) ◽  
pp. 419-428 ◽  
Author(s):  
C.R. LOVELL ◽  
K.A. SMOLENSKI ◽  
V.C. DUANCE ◽  
N.D. LIGHT ◽  
S. YOUNG ◽  
...  

1995 ◽  
Vol 25 (2) ◽  
pp. 51A-52A
Author(s):  
Ron Waksman ◽  
Keith A. Robinson ◽  
Joel Schneider ◽  
Gustavo Cipolla ◽  
Hiroyuki Masayasu ◽  
...  

2004 ◽  
Vol 5 (1) ◽  
pp. 112-113
Author(s):  
X. Cheng ◽  
M. Kuzuya ◽  
T. Sasaki ◽  
S. Kanda ◽  
T. Shibata ◽  
...  

1985 ◽  
Vol 249 (3) ◽  
pp. H540-H546 ◽  
Author(s):  
M. J. Rubin ◽  
H. G. Bohlen

The effect of chronic, severe diabetes mellitus on the morphology, blood flow regulation, and tissue PO2 of the cerebral cortex was evaluated in adult rats. The arterioles of the diabetic animals were enlarged in terms of both lumen diameter and vessel wall area. Although resting blood flow in the diabetic rats was greater than in the normal rats, the autoregulation of cerebral blood flow was very good within an arterial pressure range of 40-150 mmHg, just as in normal rats. The resting tissue PO2 in diabetic rats was 14.9 +/- 0.5 (SEM) compared with 12.7 +/- 0.6 mmHg in normal animals and in both groups remained at or near the resting PO2 at arterial pressures from 40 to 150 mmHg. There was no apparent loss of arterioles on the cortex surface or change in length of individual arterioles in diabetic animals but there was a 20-30% decrease in the number of venules and no change in the length of individual venules. These data indicate that although the arteriolar morphology and number of venules change in the brain during diabetes, physiological function in terms of tissue PO2 and blood flow regulation is maintained within normal limits.


1999 ◽  
Vol 29 (10) ◽  
pp. 1097
Author(s):  
Bong Ki Lee ◽  
Junghan Yoon ◽  
Han Hyo Lee ◽  
Jang Young Kim ◽  
Ji Yean Ko ◽  
...  

2021 ◽  
Vol 12 ◽  
Author(s):  
Shaimaa Nasr Amin ◽  
Nivin Sharawy ◽  
Nashwa El Tablawy ◽  
Dalia Azmy Elberry ◽  
Mira Farouk Youssef ◽  
...  

Diabetes mellitus (DM) is a multisystem endocrine disorder affecting the brain. Mesenchymal stem cells (MSCs) pretreated with Melatonin have been shown to increase the potency of MSCs. This work aimed to compare Melatonin, stem cells, and stem cells pretreated with Melatonin on the cognitive functions and markers of synaptic plasticity in an animal model of type I diabetes mellitus (TIDM). Thirty-six rats represented the animal model; six rats for isolation of MSCs and 30 rats were divided into five groups: control, TIDM, TIDM + Melatonin, TIDM + Stem cells, and TIDM + Stem ex vivo Melatonin. Functional assessment was performed with Y-maze, forced swimming test and novel object recognition. Histological and biochemical evaluation of hippocampal Neuroligin 1, Sortilin, Brain-Derived Neurotrophic Factor (BDNF), inducible nitric oxide synthase (iNOS), toll-like receptor 2 (TLR2), Tumor necrosis factor-alpha (TNF-α), and Growth Associated Protein 43 (GAP43). The TIDM group showed a significant decrease of hippocampal Neuroligin, Sortilin, and BDNF and a significant increase in iNOS, TNF-α, TLR2, and GAP43. Melatonin or stem cells groups showed improvement compared to the diabetic group but not compared to the control group. TIDM + Stem ex vivo Melatonin group showed a significant improvement, and some values were restored to normal. Ex vivo melatonin-treated stem cells had improved spatial working and object recognition memory and depression, with positive effects on glucose homeostasis, inflammatory markers levels and synaptic plasticity markers expression.


2020 ◽  
Vol 13 (10) ◽  
pp. 102
Author(s):  
A. V. Silva ◽  
J. F. Silva Filho ◽  
M. C. T. Silva ◽  
N. C. A. Vaz ◽  
M. L. G. Silva

Common Bean (Phaseolusvulgaris L) is one of the main food sources in Brazil, hás cultural and nutricional values. Its cultivation can be found mosthy by small producers with small production áreas and some technological types, and extends throughout the national territory. The objective of this research was to elaborate a review about the edaphoclimatic adaptability and agricultural production environments of the bean crop. Since, beans can be sown at three times of the year, in which edaphoclimatic characteristics are defined as: 1 st crop (Sowing performed between August and October); 2nd crop (Sowing performed from January to April); and autumn crop winter crop, in which sowing occurs from May and, characterized as annual crop. In the 3rd harvest the use of irrigatin system, made by sprinkler system. However, sowing in different harvest may be influenced by the type of culivar used, as it hás Type I characterization because it hás a determined growth habit with reproductive terminal buds in the main stem and branches, while the second (types II, III and IV) have indeterminate growth habit with vegetative terminal buds on the main stem and branches. However there is a difference between the three types in the plant, the type III prostrate sinze and type IV plant that hás large climbing capacity. Although it demonstrates these attributes, the bean also hás during its biological cycle the vegetative and reproductive phases, which are divided into tem stages. The vegetative phase (V) consists of stages V0, V1, V2, V3 and V4 and the reproductive phase (R), of stages R5, R6, R 7, R8 and R9 being crucial for the development of the crop. Despite these stages of bean development, it hás wide adaptation and geographical distribuion, the bean  is very little tolerant to extreme environmental factors, being a relatively demanding crop with reagard to most edaphoclimatic conditions. Thus, the agroclimatic characteristics of the region and the requirements and limitations of common bean are of fundamental importance for the choice of an environment where the crop can grow and develop well, in order to obtain optimum yield, taking full advantage of the cultivar. and other practices or technologies employed in order to provide positive effects on the crop.Common Bean (Phaseolusvulgaris L) is one of the main food sources in Brazil, hás cultural and nutricional values. Its cultivation can be found mosthy by small producers with small production áreas and some technological types, and extends throughout the national territory. The objective of this research was to elaborate a review about the edaphoclimatic adaptability and agricultural production environments of the bean crop. Since, beans can be sown at three times of the year, in which edaphoclimatic characteristics are defined as: 1 st crop (Sowing performed between August and October); 2nd crop (Sowing performed from January to April); and autumn crop winter crop, in which sowing occurs from May and, characterized as annual crop. In the 3rd harvest the use of irrigatin system, made by sprinkler system. However, sowing in different harvest may be influenced by the type of culivar used, as it hás Type I characterization because it hás a determined growth habit with reproductive terminal buds in the main stem and branches, while the second (types II, III and IV) have indeterminate growth habit with vegetative terminal buds on the main stem and branches. However there is a difference between the three types in the plant, the type III prostrate sinze and type IV plant that hás large climbing capacity. Although it demonstrates these attributes, the bean also hás during its biological cycle the vegetative and reproductive phases, which are divided into tem stages. The vegetative phase (V) consists of stages V0, V1, V2, V3 and V4 and the reproductive phase (R), of stages R5, R6, R 7, R8 and R9 being crucial for the development of the crop. Despite these stages of bean development, it hás wide adaptation and geographical distribuion, the bean  is very little tolerant to extreme environmental factors, being a relatively demanding crop with reagard to most edaphoclimatic conditions. Thus, the agroclimatic characteristics of the region and the requirements and limitations of common bean are of fundamental importance for the choice of an environment where the crop can grow and develop well, in order to obtain optimum yield, taking full advantage of the cultivar. and other practices or technologies employed in order to provide positive effects on the crop.


Endocrinology ◽  
2009 ◽  
Vol 150 (5) ◽  
pp. 2463-2471 ◽  
Author(s):  
Kyung-Bon Lee ◽  
Anilkumar Bettegowda ◽  
Gabbine Wee ◽  
James J. Ireland ◽  
George W. Smith

Previous studies established a positive relationship between oocyte competence and follistatin mRNA abundance. Herein, we used the bovine model to test the hypothesis that follistatin plays a functional role in regulation of early embryogenesis. Treatment of early embryos with follistatin during in vitro culture (before embryonic genome activation) resulted in a dose-dependent decrease in time to first cleavage, increased numbers of blastocysts, and increased blastocyst total and trophectoderm cell numbers. To determine the requirement of endogenous follistatin for early embryogenesis, follistatin ablation/replacement studies were performed. Microinjection of follistatin small interfering RNA into zygotes reduced follistatin mRNA and protein and was accompanied by a reduction in number of embryos developing to eight- to 16-cell and blastocyst stages and reduced blastocyst total and trophectoderm cell numbers. Effects of follistatin ablation were rescued by culture of follistatin small interfering RNA-injected embryos in the presence of exogenous follistatin. To investigate whether follistatin regulation of early embryogenesis is potentially mediated via inhibition of endogenous activin activity, the effects of treatment of embryos with exogenous activin, SB-431542 (inhibitor of activin, TGF-β, and nodal type I receptor signaling) and follistatin plus SB-431542 were investigated. Activin treatment mimicked positive effects of follistatin on time to first cleavage and blastocyst development, whereas negative effects of SB-431542 treatment were observed. Stimulatory effects of follistatin on embryogenesis were not blocked by SB-431542 treatment. Results support a functional role for oocyte-derived follistatin in bovine early embryogenesis and suggest that observed effects of follistatin are likely not mediated by classical inhibition of activin activity.


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