scholarly journals Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca2+-Sensing Receptor Signaling

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Xuehui Zhang ◽  
Song Meng ◽  
Ying Huang ◽  
Mingming Xu ◽  
Ying He ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Composite Nanofibers ◽  
Cell Attachment ◽  
Receptor Signaling ◽  
Porous Network ◽  
Composite Scaffolds ◽  
Electrospun Gelatin

Calcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/β-tricalcium phosphate (TCP) and confirmed their biological activityin vitroand bone regenerationin vivo. However, how these composite nanofibers promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is unknown. Here, gelatin/β-TCP composite nanofibers were fabricated by incorporating 20 wt%β-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the compositeβ-TCP nanofibers had a nonwoven structure with a porous network and a rough surface. Spectral analyses confirmed the presence and chemical stability of theβ-TCP and gelatin components. Compared with pure gelatin nanofibers, gelatin/β-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs. Interestingly, the expression level of the calcium-sensing receptor (CaSR) was significantly higher on the composite nanofibrous scaffolds than on pure gelatin. For rat calvarial critical sized defects, more extensive osteogenesis and neovascularization occurred in the composite scaffolds group compared with the gelatin group. Thus, gelatin/β-TCP composite scaffolds promote osteogenic differentiation of BMSCsin vitroand bone regenerationin vivoby activating Ca2+-sensing receptor signaling.

scholarly journals Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 853 ◽  
Author(s):  
Dewi Sartika ◽  
Chih-Hsin Wang ◽  
Ding-Han Wang ◽  
Juin-Hong Cherng ◽  
Shu-Jen Chang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Silk Fibroin ◽  
Bone Repair ◽  
Matrix Deposition ◽  
Calvarial Defects

Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (BMP-2, COL1a1, and OCN) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications.

scholarly journals Microporous polysaccharide multilayer coated BCP composite scaffolds with immobilised calcitriol promote osteoporotic bone regeneration both in vitro and in vivo

Theranostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 1125-1143 ◽  
Author(s):  
Qian Tang ◽  
Zhichao Hu ◽  
Haiming Jin ◽  
Gang Zheng ◽  
XingFang Yu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Osteoporotic Bone ◽  
Composite Scaffolds

Effects of Hydroxyapatite-Containing Composite Nanofibers on Osteogenesis of Mesenchymal Stem Cells In vitro and Bone Regeneration In vivo

2013 ◽  
Vol 5 (2) ◽  
pp. 319-330 ◽  
Author(s):  
Lan-Xin Lü ◽  
Xiao-Feng Zhang ◽  
Yan-Yan Wang ◽  
Lazarus Ortiz ◽  
Xi Mao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Composite Nanofibers

scholarly journals In Vitro and In Vivo Evaluation of Commercially Available Fibrin Gel as a Carrier of Alendronate for Bone Tissue Engineering

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Beom Su Kim ◽  
Feride Shkembi ◽  
Jun Lee
Keyword(s):  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Release Kinetics ◽  
Human Mesenchymal Stem Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Fibrin Gel ◽  
In Vivo Evaluation

Alendronate (ALN) is a bisphosphonate drug that is widely used for the treatment of osteoporosis. Furthermore, local delivery of ALN has the potential to improve the bone regeneration. This study was designed to investigate an ALN-containing fibrin (fibrin/ALN) gel and evaluate the effect of this gel on both in vitro cellular behavior using human mesenchymal stem cells (hMSCs) and in vivo bone regenerative capacity. Fibrin hydrogels were fabricated using various ALN concentrations (10−7–10−4 M) with fibrin glue and the morphology, mechanical properties, and ALN release kinetics were characterized. Proliferation and osteogenic differentiation of and cytotoxicity in fibrin/ALN gel-embedded hMSCs were examined. In vivo bone formation was evaluated using a rabbit calvarial defect model. The fabricated fibrin/ALN gel was transparent with Young’s modulus of ~13 kPa, and these properties were not affected by ALN concentration. The in vitro studies showed sustained release of ALN from the fibrin gel and revealed that hMSCs cultured in fibrin/ALN gel showed significantly increased proliferation and osteogenic differentiation. In addition, microcomputed tomography and histological analysis revealed that the newly formed bone was significantly enhanced by implantation of fibrin/ALN gel in a calvarial defect model. These results suggest that fibrin/ALN has the potential to improve bone regeneration.

scholarly journals Microporous polysaccharide multilayer coated BCP composite scaffolds with immobilised calcitriol promote osteoporotic bone regeneration both in vitro and in vivo: Erratum

Theranostics ◽  
2021 ◽  
Vol 11 (13) ◽  
pp. 6524-6525
Author(s):  
Qian Tang ◽  
Zhichao Hu ◽  
Haiming Jin ◽  
Gang Zheng ◽  
XingFang Yu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Osteoporotic Bone ◽  
Composite Scaffolds

Synergistic effects of elastic modulus and surface topology of Ti-based implants on early osseointegration

RSC Advances ◽  
2016 ◽  
Vol 6 (49) ◽  
pp. 43685-43696 ◽  
Author(s):  
Xiaohan Dai ◽  
Xuehui Zhang ◽  
Mingming Xu ◽  
Ying Huang ◽  
Boon Chin Heng ◽  
...  
Keyword(s):  
Elastic Modulus ◽  
Synergistic Effect ◽  
Osteogenic Differentiation ◽  
Cell Attachment ◽  
Synergistic Effects ◽  
Surface Topology ◽  
Ti Alloy ◽  
Micro Scale

Elastic modulus and surface micro-scale topographical structure of Ti alloy implants have a synergistic effect on cell attachment, osteogenic differentiation of rBMSCs in vitro and early osseointegration in vivo.

scholarly journals The impact of Zn-doped synthetic polymer materials on bone regeneration: a systematic review

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Siyi Wang ◽  
Rong Li ◽  
Dandan Xia ◽  
Xiao Zhao ◽  
Yuan Zhu ◽  
...  
Keyword(s):  
Systematic Review ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Synthetic Polymers ◽  
Synthetic Polymer ◽  
Polymer Materials ◽  
In Vivo Experiments

Abstract Introduction To repair bone defects, a variety of bone substitution materials have been used, such as ceramics, metals, natural and synthetic polymers, and combinations thereof. In recent decades, a wide range of synthetic polymers have been used for bone regeneration. These polymers have the advantages of biocompatibility, biodegradability, good mechanical properties, low toxicity, and ease of processing. However, when used alone, they are unable to achieve ideal bone formation. Incorporating zinc (Zn) into synthetic polymers has been considered, as previous studies have shown that Zn2+ promotes stem cell osteogenesis and mineral deposition. The purpose of this systematic review was to provide an overview of the application and effectiveness of Zn in synthetic polymers for bone regeneration, whether used alone or in combination with other biomaterials. This study was performed according to the PRISMA guidelines. Materials and methods A search of the PubMed, Embase, and the Cochrane Library databases for articles published up to June 2020 revealed 153 relevant studies. After screening the titles, abstracts, and full texts, 13 articles were included in the review; 9 of these were in vitro, 3 were in vivo, and 1 included both in vitro and in vivo experiments. Results At low concentrations, Zn2+ promoted cell proliferation and osteogenic differentiation, while high-dose Zn2+ resulted in cytotoxicity and inhibition of osteogenic differentiation. Additionally, one study showed that Zn2+ reduced apatite formation in simulated body fluid. In all of the in vivo experiments, Zn-containing materials enhanced bone formation. Conclusions At appropriate concentrations, Zn-doped synthetic polymer materials are better able to promote bone regeneration than materials without Zn.

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