Chemical and Functional Characterization of Sarcoplasmic Proteins from Giant Squid (Dosidicus gigas) Mantle

Modification of pH and NaCl concentration changed the physicochemical properties of sarcoplasmic proteins (SP) from jumbo squid mantle and consequently their functional properties. Better results of emulsifying activity index (EAI) and foam capacity (FC) were exhibited at pH 11 in NaCl absence due to higher solubility. But better emulsifying stability index (ESI) was obtained at pH 11 in 0.5 M NaCl, while, foaming stability (FS) was better at pH near to isoelectric point (pI). These results suggest that SP from jumbo squid may be a promising ingredient, whose functional properties can be manipulated by changing pH and NaCl concentration.

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Functional characterization of leucine transport induced in Xenopus laevis oocytes injected with mRNA isolated from midguts of lepidopteran larvae (Philosamia cynthia).

Journal of Experimental Biology ◽

10.1242/jeb.198.4.961 ◽

1995 ◽

Vol 198 (4) ◽

pp. 961-966

Author(s):

V F Sacchi ◽

C Perego ◽

S Magagnin

Keyword(s):

Xenopus Laevis ◽

Functional Characterization ◽

Xenopus Laevis Oocytes ◽

Fourfold Increase ◽

Leucine Uptake ◽

Lepidopteran Larvae ◽

Nacl Concentration ◽

Leucine Transport ◽

The Difference

The injection of poly(A)+ mRNA prepared from Philosamia cynthia midgut caused time- and dose-dependent increases of leucine transport in Xenopus laevis oocytes, with an increase in leucine uptake 1.5-3 times that of oocytes injected with water. When the NaCl concentration was reduced from 100 to 5 mmol l-1, the difference between mRNA- and water-injected oocytes was greater and a fourfold increase of L-leucine uptake was measured. D-Leucine (10 mmol l-1) completely inhibited the induced uptake of 0.1 mmol l-1 L-leucine. The newly expressed component of L-leucine uptake increased at alkaline pH and was abolished by incubation for 15 min with 15 mmol l-1 phenylglyoxal. The mean Km values, calculated using Na+ activation curves of leucine uptake, were 23.3 +/- 6.1 mmol l-1 in water-injected oocytes and 0.4 +/- 0.2 mmol l-1 for the newly expressed component of leucine uptake in mRNA-injected oocytes. On the basis of these results, we conclude that the increase of L-leucine uptake in mRNA-injected oocytes was due to the expression of a new transport system, which differs from the endogenous ones and shares many features with that found previously in Philosamia cynthia midgut.

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Cloning and functional characterization of a superfamily of microbial inwardly rectifying potassium channels

Physiological Genomics ◽

10.1152/physiolgenomics.00026.2006 ◽

2006 ◽

Vol 26 (1) ◽

pp. 1-7 ◽

Cited By ~ 14

Author(s):

Si Sun ◽

Jo Han Gan ◽

Jennifer J. Paynter ◽

Stephen J. Tucker

Keyword(s):

Functional Properties ◽

Functional Characterization ◽

Unicellular Eukaryote ◽

Channel Blockers ◽

E Coli ◽

Inwardly Rectifying Potassium Channels ◽

Genes Encoding ◽

Functionally Diverse ◽

Inwardly Rectifying

Our understanding of the mammalian inwardly rectifying family of K+ channels (Kir family) has recently been advanced by X-ray crystal structures of two homologous prokaryotic orthologs (KirBac1.1 and KirBac3.1). However, the functional properties of these KirBac channels are still poorly understood. To address this problem, we cloned and characterized genes encoding KirBac orthologs from a wide variety of different prokaryotes and a simple unicellular eukaryote. The functional properties of these KirBacs were then examined by growth complementation in a K+ uptake-deficient strain of Escherichia coli (TK2420). Whereas some KirBac genes exhibited robust growth complementation, others either did not complement or showed temperature-dependent complementation including KirBac1.1 and KirBac3.1. In some cases, KirBac expression was also toxic to the growth of E. coli. The KirBac family exhibited a range of sensitivity to the K+ channel blockers Ba2+ and Cs+ as well as differences in their ability to grow on very low-K+ media, thus demonstrating major differences in their permeation properties. These results reveal the existence of a functionally diverse superfamily of microbial KirBac genes and present an excellent resource for the structural and functional analysis of this class of K+ channels. Furthermore, the complementation assay used in this study provides a simple and robust method for the functional characterization of a range of prokaryotic K+ channels that are difficult to study by traditional methods.

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Decision letter for "Proteomic identification and physicochemical characterization of paramyosin and collagen from octopus ( Octopus vulgaris ) and jumbo squid ( Dosidicus gigas )"

10.1111/ijfs.14587/v1/decision1 ◽

2020 ◽

Keyword(s):

Physicochemical Characterization ◽

Octopus Vulgaris ◽

Dosidicus Gigas ◽

Jumbo Squid ◽

Proteomic Identification

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Effect of Bovine Plasma Protein on Autolysis and Gelation of Protein Extracted from Giant Squid (Dosidicus gigas) Mantle

Journal of Chemistry ◽

10.1155/2015/392728 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Laura Raquel Marquez-Alvarez ◽

Wilfrido Torres-Arreola ◽

Victor Manuel Ocano-Higuera ◽

Benjamin Ramirez-Wong ◽

Enrique Marquez-Rios

Keyword(s):

Plasma Protein ◽

Hydrophobic Interactions ◽

Dosidicus Gigas ◽

Giant Squid ◽

Jumbo Squid ◽

Gelling Properties ◽

Textural Quality ◽

Bovine Plasma ◽

Autolytic Activity

The effect of bovine plasma protein (BPP) on the inhibition of autolytic activity and its effect on the gelling properties of a protein concentrate (PC) obtained from jumbo squid (Dosidicus gigas) mantle were investigated. Sols and gels were prepared from the PC by adding different amounts of BPP (0, 1, and 2%). Dynamic oscillatory measurements indicated that systems with 1% BPP had a higher elastic modulus (G′), in which hydrophobic interactions were favored. Concerning the technological and textural quality of the gels, BPP caused a greater water holding capacity (WHC), force, cohesiveness, and elasticity, probably due to improvement of the electrostatic and hydrophobic interactions during gel formation. Scanning electron microscopy (SEM) allowed visualization of the formation of more rigid and ordered gels with less porosity when BPP was added. Therefore, the addition of BPP improved the gelling capacity of proteins extracted from giant squid.

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Partial characterization of an effluent produced by cooking of Jumbo squid (Dosidicus gigas) mantle muscle

Bioresource Technology ◽

10.1016/j.biortech.2009.08.074 ◽

2010 ◽

Vol 101 (2) ◽

pp. 600-605 ◽

Cited By ~ 8

Author(s):

Zaidy G. Rosas-Romero ◽

Juan C. Ramirez-Suarez ◽

Ramón Pacheco-Aguilar ◽

Maria E. Lugo-Sánchez ◽

Gisela Carvallo-Ruiz ◽

...

Keyword(s):

Partial Characterization ◽

Dosidicus Gigas ◽

Jumbo Squid

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Functional Properties of Protein from Frozen Mantle and Fin of Jumbo Squid Dosidicus gigas in Function of pH and Ionic Strength

Food Science and Technology International ◽

10.1177/1082013210367157 ◽

2010 ◽

Vol 16 (5) ◽

pp. 451-458 ◽

Cited By ~ 9

Author(s):

J.G. Rocha-Estrada ◽

J.H. Córdova-Murueta ◽

F.L. García-Carreno

Keyword(s):

Connective Tissue ◽

Functional Properties ◽

Salt Concentration ◽

Foam Stability ◽

High Strength ◽

Foaming Properties ◽

Dosidicus Gigas ◽

Jumbo Squid ◽

Sds Page ◽

Protein Properties

Functional properties of protein from mantle and fin of the jumbo squid Dosidicus gigas were explained based on microscopic muscle fiber and protein fractions profiles as observed in SDS-PAGE. Fin has higher content of connective tissue and complex fiber arrangement, and we observed higher hardness of fin gels as expected. Myosin heavy chain (MHC) was found in sarcoplasmic, myofibril and soluble-in-alkali fractions of mantle and only in sarcoplasmic and soluble-in-alkali fractions of fin. An additive effect of salt concentration and pH affected the solubility and foaming properties. Fin and mantle proteins yielded similar results in solubility tests, but significant differences occurred for specific pH and concentrations of salt. Foaming capacity was proportional to solubility; foam stability was also affected by pH and salt concentration. Hardness and fracture strength of fin gels were significantly higher than mantle gels; gels from proteins of both tissues reached the highest level in the folding test. Structural and molecular properties, such as MHC and paramyosin solubility, arrangement of muscle fibers and the content of connective tissue were useful to explain the differences observed in these protein properties. High-strength gels can be formed from squid mantle or fin muscle. Fin displayed similar or better properties than mantle in all tests.

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Physicochemical and Techno-Functional Characterization of Native Corn Reintroduced in the Andean Zone of Jujuy, Argentina

Proceedings ◽

10.3390/proceedings2020053007 ◽

2020 ◽

Vol 53 (1) ◽

pp. 7

Author(s):

María Alejandra Giménez ◽

Cristina Noemí Segundo ◽

Manuel Oscar Lobo ◽

Norma Cristina Sammán

Keyword(s):

Principal Component Analysis ◽

Functional Properties ◽

Food Industry ◽

Functional Characterization ◽

Principal Component ◽

Component Analysis ◽

High Variability ◽

Pca Analysis

The chemical and techno-functional properties of nine maize races from the Andean zone of Jujuy, Argentina, in the process of reintroduction, were determined. Principal component analysis (PCA) was applied to establish the differences between them. The breeds studied showed high variability in their chemical and techno-functional properties, which would indicate that their applications in the food industry will also be differentiated. The PCA analysis allowed us to group them into four groups, and the Capia Marron and Culli races showed unique properties, mainly in the formation of gels.

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