scholarly journals Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Nakul Mandal ◽  
Geoffrey P. Lewis ◽  
Steven K. Fisher ◽  
Steffen Heegaard ◽  
Jan U. Prause ◽  
...  
Keyword(s):  
Retinal Detachment ◽  
Rhegmatogenous Retinal Detachment ◽  
Photoreceptor Cell ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Differentially Expressed ◽  
Sham Surgery ◽  
Proteomic Investigation ◽  
Pigmented Rabbits ◽  
The Right

Purpose. The pathogenesis of rhegmatogenous retinal detachment (RRD) remains incompletely understood, with no clinically effective treatment for potentially severe complications such as photoreceptor cell death and proliferative vitreoretinopathy. Here we investigate the protein profile of the vitreous following experimental retinal detachment using a comparative proteomic based approach.Materials and Methods. Retinal detachment was created in the right eyes of six New Zealand red pigmented rabbits. Sham surgery was undertaken in five other rabbits that were used as controls. After seven days the eyes were enucleated and the vitreous was removed. The vitreous samples were evaluated with two-dimensional polyacrylamide gel electrophoresis and the differentially expressed proteins were identified with tandem mass spectrometry.Results. Ten protein spots were found to be at least twofold differentially expressed when comparing the vitreous samples of the sham and retinal detachment surgery groups. Protein spots that were upregulated in the vitreous following retinal detachment were identified as albumin fragments, and those downregulated were found to be peroxiredoxin 2, collagen-Iα1 fragment, andα-1-antiproteinase F.Conclusions. Proteomic investigation of the rabbit vitreous has identified a set of proteins that help further our understanding of the pathogenesis of rhegmatogenous retinal detachment and its complications.

A Case of Retinal Detachment in Retinitis Pigmentosa

2007 ◽  
Vol 17 (4) ◽  
pp. 677-679 ◽  
Author(s):  
M.N. Demir ◽  
N. Ünlü ◽  
Z. Yalniz ◽  
M.A. Acar ◽  
F. Örnek
Keyword(s):  
Retinal Detachment ◽  
Retinitis Pigmentosa ◽  
Pars Plana Vitrectomy ◽  
Rhegmatogenous Retinal Detachment ◽  
Silicone Oil ◽  
Young Patients ◽  
Scleral Buckling ◽  
Oil Removal ◽  
Pars Plana ◽  
The Right

Purpose To report case of retinitis pigmentosa in association with rhegmatogenous retinal detachment. Methods An eight year old boy complained of a sudden visual loss. The patient had night blindness, bone spicule-like hyperpigmentation, pale optic disc in both eyes, and the retina was totally detached in the right eye. Results He was initially treated with conventional scleral buckling surgery, then pars plana vitrectomy with silicone tamponade was performed and retinal reattachment was established. After the phacoemulsification combined with silicone oil removal the final visual acuity of counting fingers was obtained. Conclusions The association of retinitis pigmentosa and rhegmatogenous retinal detachment is uncommon in young patients.

Rhegmatogenous retinal detachment with giant retinal tear in a child with Marfan’s syndrome: a rare ocular emergency

2021 ◽  
Vol 14 (6) ◽  
pp. e241354
Author(s):  
Parrina Sehgal ◽  
Subina Narang ◽  
Deepak Chandra
Keyword(s):  
Retinal Detachment ◽  
Pars Plana Vitrectomy ◽  
Rhegmatogenous Retinal Detachment ◽  
Marfan’S Syndrome ◽  
Marfan's Syndrome ◽  
Retinal Tear ◽  
Giant Retinal Tear ◽  
Pars Plana ◽  
23 Gauge ◽  
The Right

A 7-year-old boy with Marfanoid habitus presented with sudden and painless decrease in the vision of the right eye. Ocular examination revealed rhegmatogenous retinal detachment with 360° giant retinal tear in the right eye and small peripheral retinal breaks with lattice degeneration in the left eye. The patient underwent a 23-gauge pars plana vitrectomy with scleral buckling in the right eye and laser around the breaks in the left eye. At 1-week follow-up visit, the child presented with similar complaints in the left eye as were seen in the right eye. This was later managed effectively with 23-gauge pars plana vitrectomy only. So, with our case report, we would like to highlight the need for aggressive screening in children who are diagnosed with Marfan’s syndrome and the need for prophylactic treatment in the unaffected eye.

scholarly journals Spontaneous Resolution of a Rhegmatogenous Retinal Detachment

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Biljana Vukadinovic-Pajovic ◽  
Miroslav Stamenkovic ◽  
Dusan Todorovic ◽  
Suncica Srecković ◽  
Vladimir Jakovljevic
Keyword(s):  
Retinal Pigment Epithelium ◽  
Retinal Detachment ◽  
Rhegmatogenous Retinal Detachment ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Vitreous Body ◽  
Tertiary Level ◽  
Ophthalmological Examination ◽  
Level Institution ◽  
The Right

Abstract A retinal detachment is the term used to describe detachment of the neurosensory retina from the underlying membrane, the retinal pigment epithelium (RPE). Rhegmatogenous detachments are caused by a break in the retina through which fluid passes from the vitreous cavity into the subretinal space. The incidence of rhegmatogenous retinal detachment in the general population in Europe is 1 in 10 000 persons per year. Danger is the greatest in the age range from 55 to 70 years. Without treatment, blindness in the affected eye may occur. Individual risks depend on the presence or absence of specific factors including myopia, positive family anamnesis, retina rupture, trauma, ablation in the other eye, ablation in a vitreous body, retina high-risk peripheral de-generations and vitreoretinal degenerations. Majority of the untreated rhegmatogenous retina ablations progress to the subtotal or total retinal detachment and blindness. This paper describes a very rare case of the spontaneous complete reattachment of the sensory retina to the retinal pigment epithelium in a patient with the total rhegmatogenous retinal ablation in the right eye. The female patient, who was 52 years old, was examined by an Ophthalmologist after she had experienced a sudden loss of vision, 2 months before appointment. After a detailed ophthalmological examination, a total rhegmatogenous retinal ablation of the right eye was diagnosed. The best corrected visual acuity, evaluated on a Snellen chart, was 2/60. The patient was referred to a tertiary-level Institution since a surgical intervention of the ablation was needed. Due to technical inabilities in the above-mentioned Institution, the operation was not performed, and despite the recommendation to perform the intervention in another tertiary-level Institution, the patient did not have ophthalmological examinations during the following three months. During the next visit, the Ophthalmologist determined that there was a spontaneous retinal fixation on the retinal pigment epithelium and a partial restoration of the visual function of the affected eye which was evaluated at 0.5.

scholarly journals Bilateral Simultaneous Rhegmatogenous Retinal Detachment following Laser in situ Keratomileusis

2016 ◽  
Vol 7 (2) ◽  
pp. 340-344 ◽  
Author(s):  
Erhan Yumusak ◽  
Kemal Ornek ◽  
Fatma Ozkal
Keyword(s):  
Retinal Detachment ◽  
Rhegmatogenous Retinal Detachment ◽  
Silicone Oil ◽  
Scleral Buckling ◽  
Laser In Situ Keratomileusis ◽  
Pars Plana ◽  
Silicone Oil Tamponade ◽  
The Right ◽  
Vitrectomy Surgery

A 21-year-old woman developed simultaneous rhegmatogenous retinal detachment after laser in situ keratomileusis (LASIK) in both eyes. She underwent pars plana vitrectomy surgery combined with endolaser photocoagulation and silicone oil tamponade in the right eye. A week later, pneumatic retinopexy was done in the left eye. As the retinal tear did not seal, 360° scleral buckling surgery was performed and retina was attached. Bilateral simultaneous rhegmatogenous retinal detachment after LASIK for correction of myopia can be a serious complication. Patients should be informed about the possibility of this complication.

scholarly journals Scleral Buckling and Corneal Topography in a Rare Case of Keratoconus with Rhegmatogenous Retinal Detachment in an Indian Patient

2018 ◽  
Vol 10 (1) ◽  
pp. 77-81
Author(s):  
B N Kalpana ◽  
Y D Shilpa ◽  
Sneha Priya Prabhakar ◽  
S M Ram Prakash ◽  
B C Hemalatha ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Retinal Detachment ◽  
Rhegmatogenous Retinal Detachment ◽  
Scleral Buckling ◽  
Corneal Topography ◽  
Vertical Meridian ◽  
History Of ◽  
Pars Plana ◽  
The Right

Background: Management of Rhegmatogenous Retinal Detachment (RRD) in keratoconus could be challenging in various aspects. Visualisation of fundus due to altered reflex along with axial myopia could pose difficulty while performing pars plana vitrectomy. Our patient underwent Scleral Buckling with good anatomical results. We came across an isolated case of Keratoconus with Retinal detachment without any pre existing comorbidities unlike earlier reports where patients with history of atopic dermatitis had Keratoconus associated with RRD. The main purpose was to know the outcome of scleral buckling and its effect on corneal topography in a case of keratoconus with RRD.Case: A 35 year old female presented with diminution of vision in both eyes since childhood, but more so in the right eye (RE) since last 6 months. She was aphakic with VA of 1/60 and 2/60 in the right and left eye respectively. She was diagnosed as both eyes keratoconus with RE near total rhegmatogenous retinal detachment (RRD) with sub retinal gliosis. She gave no history of vigorous eye rubbing or atopic dermatitis. For RE she underwent uneventful scleral buckling surgery.Observation: In post operative follow up, the retina was attached. Placido based corneal topography was done pre operatively with keratometry reading of RE – K1 62.79@96º, K2 – 55.92@6˚ and repeated at the end of three months follow up with readings of RE – K1-61.45@98˚, K2- 54.50@ 8˚. There were minimal changes in the keratometry values post operatively with flattening of vertical meridian and horizontal meridian.Conclusion: In keratoconus, RD can occur without any predisposed or preceding condition. Although majority of cases are associated with atopic dermatitis and eye rubbing. Scleral buckling (SB) was successful with good functional and anatomical outcome., however it has minimal effect on corneal topography.  

scholarly journals Scleral buckling—a brief historical overview and current indications

2019 ◽  
Vol 258 (3) ◽  
pp. 467-478
Author(s):  
Aijing Wang ◽  
Martin P. Snead
Keyword(s):  
Retinal Detachment ◽  
Rhegmatogenous Retinal Detachment ◽  
Surgical Techniques ◽  
Scleral Buckling ◽  
Effective Technique ◽  
Retinal Breaks ◽  
Retinal Tears ◽  
Pars Plana ◽  
Retinal Hole ◽  
The Right

AbstractThe key to successful management of rhegmatogenous retinal detachment (RRD) is to find and seal all of the retinal breaks, and the two main surgical techniques used to achieve this are scleral bucking (SB) or pars plana vitrectomy (PPV). Techniques for SB have remained mostly unchanged for the last 60 years, whilst PPV techniques and instruments have developed substantially over that time and have greatly contributed to increased success rate for types and configurations of retinal detachments unsuitable or difficult to manage with buckling alone. However, there is a growing dependency to rely on PPV as the sole and only approach for repair of all types of retinal detachment, such that some centres are no longer offering training in scleral buckling. There are also many studies comparing SB with PPV, but many of these lack information on the type, technique or rationale for deployment of the buckle. Many studies deploy the same scleral buckle technique without customising it to the type, position or number of tears being treated. Scleral buckling is not a one-size-fits-all technique. It requires careful patient selection and careful buckle selection and orientation tailored to the tear(s) to ensure success. When used appropriately, it is a simple and highly effective technique, particularly for retinal dialyses, round retinal hole detachments and selected cases of retinal detachment associated with horseshoe retinal tears. There is no doubt that for some more complex cases, such as multiple large breaks, giant retinal tears, bullous detachments and cases complicated by proliferative retinopathy, PPV offers a safer and more effective management. However, SB remains an important and relevant surgical technique, and for the right cases, the results can be superior to PPV with reduced comorbidity.

scholarly journals INDUCED PROTEIN PROFILE CHANGES IN ARSENATE TOLERANT AND SENSITIVE PSEUDOMONAS FLUORESCENS STRAINS

2007 ◽  
Vol 15 (4) ◽  
pp. 221-226 ◽  
Author(s):  
Stefan Shilev ◽  
Almudena Fernández López ◽  
María Sancho Prieto ◽  
Enrique David Sancho Puebla
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Toxic Metals ◽  
Statistical Significance ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Tolerant Strain ◽  
Metabolic Versatility

Pseudomonas fluorescens are gram‐negative, motile, rod‐shaped bacteria known for their metabolic versatility. Many strains of this species possess excellent capability to colonize plant roots, promoting plant growth in soils contaminated with toxic metals. P. fluorescens biotype F is a strain tolerant to arsenic which had been isolated previously from soil contaminated with arsenic and other toxic metals and characterized as a promoter of plant growth and accumulation of arsenic. In the present work we studied the protein profile of this strain together with the profile of a sensitive P. fluorescens CECT 378 in the presence and absence of sodium arsenate using two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE). The gels were analyzed by PDQuest, while the statistical significance was proved by ANOVA. We found 9 differentially expressed proteins in the tolerant strain ‐ 4 new proteins, 4 upregulated proteins and 1 downregulated proteins in the presence of 1000 ppm As. In the non‐tolerant strain there were 7 differentially expressed proteins ‐ 1 new protein, 3 upregulated proteins and 3 downregulated proteins. The identification of the proteins with MALDI‐TOF is in progress.

scholarly journals Two Cases of Giant Melanocytoma (Hyperpigmented Magnocellular Nevus)

2020 ◽  
Vol 6 (5) ◽  
pp. 344-352
Author(s):  
David I.T. Sia ◽  
Jorge Agi ◽  
Parampal Grewal ◽  
Laurie Russell ◽  
Ezekiel Weis
Keyword(s):  
Optic Nerve ◽  
Retinal Detachment ◽  
Ciliary Body ◽  
Rhegmatogenous Retinal Detachment ◽  
Vitreous Hemorrhage ◽  
Melanocytic Nevus ◽  
First Case ◽  
Iodine 125 ◽  
The Right ◽  
Plaque Brachytherapy

Melanocytoma or hyperpigmented magnocellular nevus is a variant of melanocytic nevus that is most commonly seen in the optic nerve, but has also been reported to occur in the iris, ciliary body, choroid, sclera, and conjunctiva. We present two cases of giant uveal melanocytoma with histopathology. The first case occurred in a 10-year-old girl who presented with decreased vision in the right eye and a mushroom-shaped pigmented choroidal lesion measuring 15.5 mm in apical height. The lesion was abutting the lens but not causing a cataract. This was diagnosed as a choroidal melanocytoma on open scleral window biopsy. The second case was in a 68-year-old lady, referred for a left nasal pigmented choroidal lesion measuring 8 mm in apical height and having a mushroom configuration. The lesion grew to 8.6 mm in height and was complicated by a vitreous hemorrhage and rhegmatogenous retinal detachment and was treated with iodine-125 plaque brachytherapy. Subsequently, the treated eye became a painful phthisical eye and was enucleated. Histopathology confirmed melanocytoma with extrascleral extension but without malignant transformation. Features of melanocytoma and other very large cases reported in the literature are discussed.

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