Oxidative Stress Parameters in Saliva and Its Association with Periodontal Disease and Types of Bacteria

Objective. To determine the association between oxidative stress parameters with periodontal disease, bleeding, and the presence of different periodontal bacteria.Methods. A cross-sectional study in a sample of eighty-six patients, divided into three groups depending on their periodontal status. Thirty-three with chronic periodontitis, sixteen with gingivitis, and thirty-seven with periodontal healthy as control. Oxidative stress biomarkers (8-OHdG and MDA), total antioxidant capacity (TAOC), and the activity of two antioxidant enzymes (GPx and SOD) were determined in saliva. Subgingival plaque samples were obtained from the deepest periodontal pocket and PCR was used to determine the presence of the 6 fimA genotypes ofPorphyromonas gingivalis,Aggregatibacter actinomycetemcomitans,Tannerella forsythia, andTreponema denticola.Results. Periodontal disease was found to be associated with increased oxidative stress parameter levels. These levels rose according to the number and type of different periodontal bacteria found in the periodontal pockets. The presence of different types of periodontal bacteria is predictive independent variables in linear regresion models of oxidative stress parameters as dependent variable, above all 8-OHdG.Conclusions. Oxidative stress parameter levels are correlated with the presence of different types of bacteria. Determination of these levels and periodontal bacteria could be a potent tool for controlling periodontal disease development.

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Increased salivary oxidative stress parameters in patients with type 2 diabetes: Relation with periodontal disease

Endocrinología Diabetes y Nutrición (English ed ) ◽

10.1016/j.endien.2017.03.010 ◽

2017 ◽

Vol 64 (5) ◽

pp. 258-264 ◽

Cited By ~ 2

Author(s):

Carlos Arana ◽

Ana María Moreno-Fernández ◽

Gerardo Gómez-Moreno ◽

Cristóbal Morales-Portillo ◽

Isabel Serrano-Olmedo ◽

...

Keyword(s):

Oxidative Stress ◽

Type 2 Diabetes ◽

Periodontal Disease ◽

Oxidative Stress Parameters ◽

Stress Parameters

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Coadministration of lithium and celecoxib reverses manic-like behavior and decreases oxidative stress in a dopaminergic model of mania induced in rats

Translational Psychiatry ◽

10.1038/s41398-019-0637-9 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Samira S. Valvassori ◽

Paula T. Tonin ◽

Gustavo C. Dal-Pont ◽

Roger B. Varela ◽

José Henrique Cararo ◽

...

Keyword(s):

Oxidative Stress ◽

Frontal Cortex ◽

Oxidative Stress Biomarkers ◽

Oxidative Stress Parameters ◽

Stress Biomarkers ◽

Novel Approach ◽

Male Wistar Rats ◽

And Oxidative Stress ◽

Cerebral Structures ◽

Stress Parameters

Abstract The present study intends to investigate the effect of lithium (Li) and celecoxib (Cel) coadministration on the behavioral status and oxidative stress parameters in a rat model of mania induced by dextroamphetamine (d-AMPH). Male Wistar rats were treated with d-AMPH or saline (Sal) for 14 days; on the 8th day of treatment, rats received lithium (Li), celecoxib (Cel), Li plus Cel, or water until day 14. Levels of oxidative stress parameters were evaluated in the serum, frontal cortex, and hippocampus. d-AMPH administration induced hyperlocomotion in rats, which was significantly reversed by Li and Cel coadministration. In addition, d-AMPH administration induced damage to proteins and lipids in the frontal cortex and hippocampus of rats. All these impairments were reversed by treatment with Li and/or Cel, in a way dependent on cerebral area and biochemical analysis. Li and Cel coadministration reversed the d-AMPH-induced decrease in catalase activity in cerebral structures. The activity of glutathione peroxidase was decreased in the frontal cortex of animals receiving d-AMPH, and treatment with Li, Cel, or a combination thereof reversed this alteration in this structure. Overall, data indicate hyperlocomotion and alteration in oxidative stress biomarkers in the cerebral structures of rats receiving d-AMPH. Li and Cel coadministration can mitigate these modifications, comprising a potential novel approach for BD therapy.

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Diet Quality Is Associated with Serum Antioxidant Capacity in Women with Breast Cancer: A Cross Sectional Study

Nutrients ◽

10.3390/nu13010115 ◽

2020 ◽

Vol 13 (1) ◽

pp. 115

Author(s):

Luiza K. Reitz ◽

Sheyla de L. Baptista ◽

Elaine da S. Santos ◽

Patrícia F. Hinnig ◽

Gabriele Rockenbach ◽

...

Keyword(s):

Breast Cancer ◽

Oxidative Stress ◽

Adjuvant Treatment ◽

Diet Quality ◽

Cell Injury ◽

Healthy Eating Index ◽

Oxidative Stress Biomarkers ◽

Oxidative Stress Parameters ◽

And Oxidative Stress ◽

Stress Parameters

Oxidative stress produced by adjuvant treatments is associated with cell injury; however, a healthy diet can help mitigate it. The aim of this study is to investigate the association between diet quality and oxidative stress parameters in women subjected to adjuvant treatment for breast cancer. The sample comprised 70 women. Oxidative stress biomarkers and diet quality parameters based on the Brazilian Healthy Eating Index—Revised (BHEI-R)—were evaluated at baseline (p0) and after adjuvant treatment (p1). Ferric reducing antioxidant potential (FRAP) was associated with diet quality at p0. BHEI-R scores were not different between p0 and p1; however, scores from total vegetables, total fruits, milk and dairy products, and meat, eggs and legumes were lower during treatment. On the other hand, lower sodium and saturated fat intake observed at p1 counterbalanced the BHEI-R score. Oxidative stress parameters have increased at p1, but they were not associated with diet quality; thus, changes in component intake were not enough to promote changes in oxidative stress during treatment. It appears that diet can enhance patients’ antioxidant defense before treatment, which could lead to better outcomes in the long term. Further investigations may help to clarify the association between diet and oxidative stress in women with breast cancer.

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The Evaluation of Saliva Oxidative and Antioxidative Markers’ Levels in Adolescents with Gingival Inflammation

Serbian Journal of Experimental and Clinical Research ◽

10.2478/sjecr-2019-0005 ◽

2019 ◽

Vol 0 (0) ◽

Author(s):

Vesna Obradovic

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Periodontal Disease ◽

Control Group ◽

World Population ◽

Oral Diseases ◽

Gingival Inflammation ◽

Oxidative Stress Parameters ◽

Whole Saliva ◽

Stress Parameters

Abstract Periodontal disease is highly prevalent and affects 10%–15% of the world population. Salivary markers of oxidative stress and antioxidant status represent promising tool for research of oral diseases. Given the importance of reactive oxygen species (ROS) in the pathogenesis of periodontal disease, the aim of this study was to determine the association between oxidative stress parameters and periodontal disease gingivitis in adolescents. The study included a consecutive sample of 80 male and female participants referred to the Institute of Dentistry, Kragujevac, Serbia, as a result of periodontal problems or for routine control, aged 18 to 21, with definitive dentition as an inclusion criterion. Patients were divided into three groups depending on their gingival inflammation status. The first group was control group (n=20) with no gingival inflammation, the second group was with mild gingival inflammation (n=19), the third group was with moderate gingival inflammation (n=21) and the fourth group was with severe gingival inflammation (n=20). Oxidative stress parameters were measured in unstimulated whole saliva samples (superoxide anion radical, hydrogen peroxide, nitric oxide, index of lipid peroxidation, reduced gluthatione, catalase and superoxide dismutase). We have found increased levels of hydrogen peroxide and reduced glutathione in the saliva of patients with moderate levels of gingival inflammation, while the other markers were not significantly affected. In conclusion, oxidative stress plays a central role in the pathogenesis and the determination of oxidative and antioxidative levels could be a potent tool in controlling the development of gingivitis.

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