Serum Levels of Stress Hormones and Oxidative Stress Biomarkers Differ according to Sasang Constitutional Type

Objectives.This study investigated whether Sasang constitutional type is associated with differences in the serum levels of stress hormones and oxidative stress.Methods.A total of 236 participants (77 males and 159 females) were enrolled. The serum levels of cortisol, adrenaline, reactive oxygen species (ROS), and malondialdehyde (MDA) were analyzed.Results.The distribution of Sasang constitutional types was as follows: Taeumin, 35.6%; Soumin, 33.0%; and Soyangin, 31.4%. The serum cortisol levels of Taeumin were significantly lower than Soumin (p<0.1in both sexes) and Soyangin (p<0.05in males andp<0.1in females). The adrenaline levels were also significantly lower in Taeumin than in Soumin (p<0.05in males andp<0.1in females) and Soyangin (p<0.1in males). Serum ROS levels were significantly higher in Soyangin than in Taeumin and Soumin (p<0.05in males), whereas MDA levels were significantly lower in Taeumin compared with Soumin and Soyangin (p<0.05in males andp<0.1in females).Conclusion.Taeumin type may tolerate psychological or oxidative stress better than other types, which suggests a biological mechanism to explain the different pathophysiological features of Sasang constitutional types.

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Changes of Inflammatory and Oxidative Stress Biomarkers in Dogs with Different Stages of Heart Failure

10.21203/rs.3.rs-24928/v1 ◽

2020 ◽

Author(s):

Camila Peres RUBIO ◽

Ahmet SARIL ◽

Meriç KOCATURK ◽

Ryou TANAKA ◽

Jorgen KOCH ◽

...

Keyword(s):

Oxidative Stress ◽

Heart Failure ◽

Acute Phase Proteins ◽

Staging System ◽

Serum Levels ◽

Left Ventricular ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Anti Oxidant ◽

And Oxidative Stress

Abstract Background: Heart failure (HF) has been associated with changes in inflammatory and oxidative-stress biomarkers. This study aimed to evaluate the changes of a panel of inflammatory and oxidative stress biomarker in dogs with different stages of HF and its relation with the severity of the disease and the echocardiographic changes. Dogs with HF as a result of myxomatous mitral valve degeneration or dilated cardiomyopathy were included. A total of 29 dogs were classified as stage-A (healthy), B (asymptomatic dogs), C (symptomatic dogs) and D (dogs with end-stage-HF) according to the ACVIM-staging-system. Serum cytokines, and inflammatory and oxidative stress markers were evaluated.Results: KC-like as an inflammatory cytokine was significantly increased in dogs of stage-C (P < 0.01) and -D (P < 0.05) compared with stage-A and -B. Stage-D Dogs showed significantly higher serum CRP and Hp (P < 0.05) but lower serum anti-oxidant capacity (PON1, TEAC, CUPRAC, and thiol) compared to stage-A and -B (P < 0.05). After the treatment, serum levels of CRP, Hp and KC-like decreased but serum anti-oxidant levels increased compared to their pre-treatment values. Left ventricular dimension and LA/Ao ratio correlated positively with CRP, MCP-1, and KC-like but negatively with PON1, GM-CSF, IL-7 and antioxidant biomarkers (P < 0.01).Conclusion: our results indicated that dogs with advanced-stages of HF show increases in positive acute-phase proteins and selected inflammatory cytokines (KC-like), and decreases in antioxidant biomarkers, indicating that inflammation and oxidative-stress act as collaborative partners in the pathogenesis of HF. KC-like may be a more useful parameter to monitor disease progression and treatment efficacy of dogs with severe-HF.

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Determination of Cytokines and Oxidative Stress Biomarkers in Cognitive Impairment Induced by Methylmalonic Acidemia

NeuroImmunoModulation ◽

10.1159/000511590 ◽

2021 ◽

Vol 28 (3) ◽

pp. 178-186

Author(s):

Qiliang Li ◽

Hong Jin ◽

Ying Liu ◽

Yu Rong ◽

Tana Yang ◽

...

Keyword(s):

Oxidative Stress ◽

Cognitive Impairment ◽

Methylmalonic Acid ◽

Serum Levels ◽

High Concentration ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Tnf Α ◽

Iq Scores ◽

And Oxidative Stress

Objective: Methylmalonic acidemia (MMA) is the most common organic acidemia in children. Many patients with MMA suffered from cognitive impairments. The aim of this study was to identify the significance of cytokines and oxidative stress biomarkers in MMA-induced cognitive impairment. Methods: We enrolled 64 children with combined MMA and homocystinuria and 64 age- and sex-matched healthy volunteers. Participants were subsequently classified as with or without cognitive impairments using a uniform neuropsychological assessment test. Serum samples were collected. The serum levels of cytokines and oxidative stress biomarkers were measured using the ELISA or chemical methods. Results: Compared to control group, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, malondialdehyde (MDA), and nitric oxide (NO) in the MMA patients increased markedly (p < 0.05); glutathione (GSH) and superoxide dismutase (SOD) decreased obviously (p < 0.01). The levels of IL-6, TNF-α, NO, and MDA in the serum were negatively associated with DQ or IQ scores. The levels of GSH and SOD in the serum were positively correlated with DQ or IQ scores. After receiver operating characteristic curve analysis, NO was the most useful individual marker for distinguishing the cognitive dysfunction, corresponding to the area under ROC curve (AUC) of 0.82 (95% CI, 0.74–0.91), sensitivity of 76.60%, and specificity of 80.25%. GSH and MDA were also useful for diagnosis of MMA-induced cognitive dysfunction, corresponding to the AUC of 0.80 (95% CI, 0.70–0.89), and 0.73 (95% CI, 0.63–0.82), respectively. The sensitivity and specificity of GSH were 72.34 and 80.25%, respectively. The sensitivity and specificity of MDA were 85.11 and 51.85%, respectively. Conclusions: The high-concentration methylmalonic acid in the blood induced immune cells to release pro-inflammatory cytokines such as TNF-α and IL-6. These cytokines and high-concentration methylmalonic acid stimulated the immune cells to produce reactive oxygen species (ROS) and reactive nitrogen species (RNS). The serum methylmalonic acid, cytokines, ROS, and RNS were across the blood-brain barrier and induced cognitive impairment. The small molecule substances such as serum NO, MDA, and GSH participated in the process of neuroinflammation and oxidative stress injury induced by MMA and could be useful for distinguishing the cognitive impairment.

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Changes of inflammatory and oxidative stress biomarkers in dogs with different stages of heart failure

BMC Veterinary Research ◽

10.1186/s12917-020-02650-7 ◽

2020 ◽

Vol 16 (1) ◽

Author(s):

Camila Peres Rubio ◽

Ahmet Saril ◽

Meriç Kocaturk ◽

Ryou Tanaka ◽

Jorgen Koch ◽

...

Keyword(s):

Oxidative Stress ◽

Heart Failure ◽

Acute Phase Proteins ◽

Staging System ◽

Serum Levels ◽

Left Ventricular ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Severity Of The Disease ◽

And Oxidative Stress

Abstract Background Heart failure (HF) is associated with changes in inflammatory and oxidative stress biomarkers. This study aimed to evaluate the changes of a panel of inflammatory and oxidative stress biomarkers in dogs with different stages of HF and its relation with the severity of the disease and echocardiographic changes. A total of 29 dogs with HF as a result of myxomatous mitral valve degeneration or dilated cardiomyopathy were included and classified as stage-A (healthy), B (asymptomatic dogs), C (symptomatic dogs) and D (dogs with end-stage HF) according to the ACVIM staging system. In these dogs an ecnhocardiographic examination was performed and cytokines, and inflammatory and oxidative stress markers were evaluated in serum. Results KC-like was significantly increased in dogs of stage-C (P < 0.01) and -D (P < 0.05) compared with stage-A and -B. Stage-D dogs showed significantly higher serum CRP and Hp (P < 0.05) but lower serum antioxidant capacity (PON1, TEAC, CUPRAC, and thiol) compared to stage-A and -B (P < 0.05). After the treatment, serum levels of CRP, Hp and KC-like decreased and serum antioxidant levels increased compared to their pre-treatment values. Left ventricular dimension and LA/Ao ratio correlated positively with CRP, MCP-1, and KC-like but negatively with PON1, GM-CSF, IL-7 and antioxidant biomarkers (P < 0.01). Conclusion Our results showed that dogs with advanced HF show increases in positive acute-phase proteins and selected inflammatory cytokines such as KC-like, and decreases in antioxidant biomarkers, indicating that inflammation and oxidative stress act as collaborative partners in the pathogenesis of HF. Some of these biomarkers of inflammation and oxidative stress could have the potential to be biomarkers to monitor the severity of the disease and the effect of treatment.

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