scholarly journals Evaluation ofCandidaColonization and Specific Humoral Responses againstCandida albicansin Patients with Atopic Dermatitis

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Ghaffari Javad ◽  
Mehdi Taheri Sarvtin ◽  
Mohammad Taghi Hedayati ◽  
Zohreh Hajheydari ◽  
Jamshid Yazdani ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Serum Levels ◽  
Rrna Gene ◽  
D2 Domain ◽  
Significant Difference ◽  
26S Rrna ◽  
Humoral Responses ◽  
Candidal Colonization

The aim of this study was to assess the candidal colonization and specific humoral responses againstCandida albicansin patients with atopic dermatitis. One hundred patients with atopic dermatitis and 50 healthy individuals were enrolled in the study. Skin and oral specimens from all participants were cultured on CHROMagarCandidamedium. Isolated yeasts were identified by using the sequence of the D1/D2 domain of the 26S rRNA gene. ELISA was used for detection of IgM, IgA, and IgG antibodies againstC. albicansin sera of participants.Candidaspecies were isolated from the skin and oral cavity of 31% of the patients and 12% of the controls. There was no significant difference betweenCandidacolonization in patients and controls (P>0.05).Candida albicanswas isolated from the skin and oral cavity of 23% of the patients and 6% of the controls (P< 0.05). There were no significant differences between serum levels of IgM and IgA in patients and controls (P>0.05). Serum level of IgG was significantly lower in patients than in controls (P<0.05). Type ofCandidacolonization can change in patients with atopic dermatitis. In addition, these patients have abnormalities in the production of antibodies againstCandida albicansthat may have a role in the pathogenesis of atopic dermatitis.Corrigendum to “Evaluation ofCandidaColonization and Specific Humoral Responses againstCandida albicansin Patients with Atopic Dermatitis”

scholarly journals Candida tibetensis sp. nov. and Candida linzhiensis sp. nov., novel anamorphic, ascomycetous yeast species from Tibet

2006 ◽  
Vol 56 (5) ◽  
pp. 1153-1156 ◽  
Author(s):  
Zuo-Wei Wu ◽  
Feng-Yan Bai
Keyword(s):  
Novel Species ◽  
Physiological Property ◽  
Rrna Gene ◽  
Ascomycetous Yeast ◽  
Domain Sequence ◽  
Yeast Strains ◽  
D2 Domain ◽  
Respective Type ◽  
26S Rrna ◽  
Type Strains

Three anamorphic, ascomycetous yeast strains isolated from plant samples collected in Linzhi District, Tibet, China, were revealed as representing two novel species by 26S rRNA gene D1/D2 domain sequence and physiological property comparisons. The names Candida tibetensis sp. nov. and Candida linzhiensis sp. nov. are proposed for these novel species, with XZ 41-6T (=AS 2.3072T=CBS 10298T) and XZ 92-1T (=AS 2.3073T=CBS 10299T) as the respective type strains. D1/D2 sequence analysis showed that C. tibetensis and C. linzhiensis are closely related to Candida caryicola and Candida sequanensis, respectively.

scholarly journals MOLECULAR IDENTIFICATION OF YEAST OF THE PULQUE BY PCR-DGGE, A TRADITIONAL MEXICANBEVERAGE

2015 ◽  
Vol 3 (3) ◽  
pp. 1-15
Author(s):  
Marcial-Quino J. ◽  
Garcia-Ocón B. ◽  
Mendoza-Espinoza J.A. ◽  
Gómez-Manzo S. ◽  
Sierra-Palacios E
Keyword(s):  
Gel Electrophoresis ◽  
Fermentation Process ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Rapid Identification ◽  
Rrna Gene ◽  
Beverage Samples ◽  
D2 Domain ◽  
Gradient Gel Electrophoresis ◽  
26S Rrna

Currently it is well known that yeasts play an essential role in the production of different beverages. In this paper, were identified some of the yeasts involved in the fermentation process of the pulque, a Mexican traditional beverage. Samples were collected from different regions of Mexico and yeasts were detected directly from samples without cultivation. Identifying the yeasts was obtained using amplification the D1/D2 domain of the 26S rRNA gene and Denaturing Gradient Gel Electrophoresis (DGGE). The results of DGGE showed different profiles of bands in each of the analyzed samples, indicating the presence of several species of yeast, which was also confirmed by sequencing of the bands corresponding to the domain D1/D2, succeeded in identifying five species of yeasts. The results obtained in this work demonstrated that the technique used for identification of yeasts of pulque was efficient. Besides, the optimization of this method could also allow rapid identification of yeasts and help understand the role of these in the fermentation process of this beverage, as well as the isolation of strains of interest for biotechnological purposes such as production of ethanol or metabolites with nutraceutical activity.

Naumovozyma baii sp. nov., an ascomycetous yeast species isolated from rotten wood in a tropical forest

2012 ◽  
Vol 62 (Pt_12) ◽  
pp. 3095-3098 ◽  
Author(s):  
Wan-Qiu Liu ◽  
Pei-Jie Han ◽  
Jun-Zhi Qiu ◽  
Qi-Ming Wang
Keyword(s):  
Gene Sequence ◽  
Yeast Species ◽  
Morphological Characteristics ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
Ascomycetous Yeast ◽  
Rotten Wood ◽  
D2 Domain ◽  
26S Rrna

Two strains isolated from rotten wood were included in the Saccharomyces group based on morphological characteristics. However, rRNA gene sequence analyses (including the 18S rRNA gene, 26S rRNA gene D1/D2 domain and internal transcribed spacer region) indicated that these two strains represent a novel species of Naumovozyma, for which the name Naumovozyma baii sp. nov. is proposed (type strain: BW 22T = CGMCC 2.04520T = CBS 12642T). The MycoBank number of the new species is MB800484.

Wickerhamomyces ochangensis sp. nov., an ascomycetous yeast isolated from the soil of a potato field

2011 ◽  
Vol 61 (10) ◽  
pp. 2543-2546 ◽  
Author(s):  
Kee-Sun Shin ◽  
Kyung-Sook Bae ◽  
Kang Hyun Lee ◽  
Doo-Sang Park ◽  
Gi-Seok Kwon ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Potato Field ◽  
Rrna Gene Sequence ◽  
Ascomycetous Yeast ◽  
D2 Domain ◽  
26S Rrna ◽  
Large Subunit Rrna

A novel ascomycetous yeast, designated strain N7a-Y2T, was isolated from soil collected in a potato field in Ochang, Korea, and its taxonomic position was studied. A neighbour-joining tree based on the D1/D2 domain of large-subunit rRNA gene sequences revealed that the isolate was a member of the Wickerhamomyces clade and that it was closely related to Wickerhamomyces bisporus, Candida quercuum, Candida ulmi and Wickerhamomyces alni. Strain N7a-Y2T formed Saturn-shaped ascospores in unconjugated and persistent asci. D1/D2 domain 26S rRNA gene sequence divergences of 11.0–21.1 % between strain N7a-Y2T and other members of the Wickerhamomyces clade indicate that the strain represents a novel species of the genus Wickerhamomyces, for which the name Wickerhamomyces ochangensis sp. nov. is proposed. The type strain is N7a-Y2T ( = KCTC 17870T  = CBS 11843T).

scholarly journals Re-examination of strains formerly assigned to Hyphopichia burtonii, the phylogeny of the genus Hyphopichia, and the description of Hyphopichia pseudoburtonii sp. nov.

2010 ◽  
Vol 60 (11) ◽  
pp. 2675-2680 ◽  
Author(s):  
Marizeth Groenewald ◽  
Maudy Th. Smith
Keyword(s):  
Internal Transcribed Spacer ◽  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Phylogenetic Groups ◽  
D2 Domain ◽  
Nucleotide Divergence ◽  
26S Rrna

On the basis of the nucleotide divergence in the internal transcribed spacer (ITS) domain of the rRNA gene and the D1/D2 domain of the 26S rRNA gene, species of the genus Hyphopichia can be divided into different phylogenetic groups. In this study, three distinct phylogenetic clusters as well as a novel species, Hyphopichia pseudoburtonii sp. nov. with type strain CBS 2455T (=JCM 16346T), are introduced. Mating studies indicate that strains belonging to Hyphopichia pseudoburtonii are heterothallic.

scholarly journals Adhesion Properties, Biofilm Forming Potential, and Susceptibility to Disinfectants of Contaminant Wine Yeasts

2021 ◽  
Vol 9 (3) ◽  
pp. 654
Author(s):  
Giorgia Perpetuini ◽  
Alessio Pio Rossetti ◽  
Noemi Battistelli ◽  
Giuseppe Arfelli ◽  
Rosanna Tofalo
Keyword(s):  
Stainless Steel ◽  
Restriction Analysis ◽  
Rrna Gene ◽  
Pichia Kudriavzevii ◽  
Adhesion Properties ◽  
Pichia Membranifaciens ◽  
Potassium Metabisulphite ◽  
D2 Domain ◽  
Pichia Manshurica ◽  
26S Rrna

In this study, yeasts isolated from filter membranes used for the quality control of bottled wines were identified and tested for their resistance to some cleaning agents and potassium metabisulphite, adhesion to polystyrene and stainless-steel surfaces, and formation of a thin round biofilm, referred to as a MAT. A total of 40 strains were identified by rRNA internal transcribed spacer (ITS) restriction analysis and sequence analysis of D1/D2 domain of 26S rRNA gene. Strains belong to Pichia manshurica (12), Pichia kudriavzevii (9), Pichia membranifaciens (1), Candida sojae (6), Candida parapsilosis (3), Candida sonorensis (1), Lodderomyces elongisporus (2), Sporopachydermia lactativora (3), and Clavispora lusitaniae (3) species. Regarding the adhesion properties, differences were observed among species. Yeasts preferred planktonic state when tested on polystyrene plates. On stainless-steel supports, adhered cells reached values of about 6 log CFU/mL. MAT structures were formed only by yeasts belonging to the Pichia genus. Yeast species showed different resistance to sanitizers, with peracetic acid being the most effective and active at low concentrations, with minimum inhibitory concentration (MIC) values ranging from 0.08% (v/v) to 1% (v/v). C. parapsilosis was the most sensible species. Data could be exploited to develop sustainable strategies to reduce wine contamination and establish tailored sanitizing procedures.

scholarly journals Polyphasic re-examination of Yarrowia lipolytica strains and the description of three novel Candida species: Candida oslonensis sp. nov., Candida alimentaria sp. nov. and Candida hollandica sp. nov.

2007 ◽  
Vol 57 (10) ◽  
pp. 2426-2435 ◽  
Author(s):  
Ann Kristin Knutsen ◽  
V. Robert ◽  
G. A. Poot ◽  
Wendy Epping ◽  
Marian Figge ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Mating Type ◽  
Type Strain ◽  
Sequence Data ◽  
Type System ◽  
Sensu Stricto ◽  
Rrna Gene ◽  
Dna Reassociation ◽  
D2 Domain ◽  
26S Rrna

The type strain of Yarrowia lipolytica and 38 strains identified as Yarrowia lipolytica, four strains of Candida deformans, including the type and two subcultures of the type, two strains of Candida galli and six unidentified strains that resembled Y. lipolytica were examined by PCR fingerprints using primers M13 and (GAC)5. The same strains, together with four strains of the recently introduced Candida yakushimensis nom. inval., were sequenced for the D1/D2 domain of the 26S rRNA gene and parts of the ITS domain and also studied for their physiological properties. Of the strains identified previously as Y. lipolytica, CBS 2076 had the same fingerprint as the type of C. deformans and strain CBS 4855 was distinct from all other strains. The six strains that resembled Y. lipolytica were separated into two groups distinct from any of the other clades. A total of six groups obtained by fingerprint and sequence data were evaluated by performing DNA reassociation reactions. Mating experiments among the 35 strains of Y. lipolytica sensu stricto showed that 15 strains represented one mating type and 16 strains represented the opposite mating type, while four strains were self-sporulating. Teleomorph states were not produced by C. deformans, C. galli or any of the unidentified isolates. However, positive mating reactions were rarely observed in crosses among C. galli and some strains of Y. lipolytica and C. deformans. Consequently, sharing the same mating type system, C. deformans and C. galli could be considered anamorphs of unnamed Yarrowia species. Results from PCR fingerprints, sequencing and mating studies support the grouping of the studied strains into Y. lipolytica, C. galli, C. deformans, C. yakushimensis nom. inval. and three novel species in the Yarrowia clade: Candida oslonensis sp. nov. (type strain CBS 10146T =NRRL Y-48252T; Mycobank number MB 510769), Candida alimentaria sp. nov. (type strain CBS 10151T =NRRL Y-48253T; Mycobank number MB 510770) and Candida hollandica sp. nov. (type strain CBS 4855T =NRRL Y-48254T; Mycobank number MB 510771).

Five novel Wickerhamomyces- and Metschnikowia-related yeast species, Wickerhamomyces chaumierensis sp. nov., Candida pseudoflosculorum sp. nov., Candida danieliae sp. nov., Candida robnettiae sp. nov. and Candida eppingiae sp. nov., isolated from plants

2011 ◽  
Vol 61 (8) ◽  
pp. 2015-2022 ◽  
Author(s):  
Marizeth Groenewald ◽  
Vincent Robert ◽  
Maudy Th Smith
Keyword(s):  
Yeast Species ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
D2 Domain ◽  
26S Rrna

On the basis of nucleotide divergences in the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacers (ITS) domain of the rRNA gene, five novel yeast species, Wickerhamomyces chaumierensis sp. nov. (CBS 8565T  = JCM 17246T), Candida pseudoflosculorum sp. nov. (CBS 8584T  = JCM 17242T), Candida danieliae sp. nov. (CBS 8533T  = JCM 17247T), Candida robnettiae sp. nov. (CBS 8580T  = JCM 17243T) and Candida eppingiae sp. nov. (CBS 8586T  = JCM 17241T), isolated from plants in Thailand and Guyana, are proposed in this study.

SDS-PAGE protein profile of Candida albicans isolated from patients with atopic dermatitis

2016 ◽  
Vol 6 (3) ◽  
pp. 92-94
Author(s):  
Kamali M. ◽  
Taheri Sarvtin M.
Keyword(s):  
Atopic Dermatitis ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecylsulfate ◽  
Dimorphic Fungus ◽  
Mucosal Surfaces ◽  
Ige Mediated ◽  
Clinical Conditions

Candida albicans is a dimorphic fungus that exists as a normal flora of hu-mans. It colonizes mucosal surfaces of the digestive tract, oral cavity and genitourinary tract and is also able to cause a variety of infections, depend-ing on the nature of the underlying host defect. This microorganism can ex-acerbate clinical conditions of atopic dermatitis via protein Allergens. The aims of this study were evaluation of protein profile of C. albicans in patients with atopic dermatitis. Four isolates of Candida albicans were collected from oral cavity of patients with atopic dermatitis. Proteins of Candida albicans were separated and reduced by sodium dodecylsulfate polyacrylamide gel electrophoresis. The extracts obtained from various isolates of Candida albicans had 62 protein bands, which ranged from 11 to >180 kDa. Sixteen different types of protein were identified. The maximum number of bands was observed in isolate B. The results of this study indicated that Candida albicans consisted of various somatic proteins that can trigger IgE-mediated reaction and exacerbation of atopic dermatitis.

scholarly journals Candida saraburiensis sp. nov. and Candida prachuapensis sp. nov., xylose-utilizing yeast species isolated in Thailand

2011 ◽  
Vol 61 (2) ◽  
pp. 462-468 ◽  
Author(s):  
Sukanya Nitiyon ◽  
Chanita Boonmak ◽  
Somjit Am-In ◽  
Sasitorn Jindamorakot ◽  
Hiroko Kawasaki ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Estuarine Water ◽  
D2 Domain ◽  
Large Subunit Rrna

Four strains of two novel xylose-utilizing yeast species were obtained from samples collected in Thailand from decaying corncobs (strains KU-Xs13T and KU-Xs18), a decaying grass (KU-Xs20) and estuarine water from a mangrove forest (WB15T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 domain of the large subunit rRNA gene, the four strains were found to represent two novel species of the genus Candida in the Candida albicans/Lodderomyces elongisporus clade. Three strains (KU-Xs13T, KU-Xs18 and KU-Xs20) were assigned as a single novel species, which was named Candida saraburiensis sp. nov. The type strain is KU-Xs13T (=CBS 11696T=NBRC 106721T=BCC 39601T). Strain WB15T represented another novel species of the genus Candida that was named Candida prachuapensis sp. nov. The type strain is WB15T (=CBS 11024T=NBRC 104881T=BCC 29904T).

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