Optimization ofArundo donaxSaccharification by (Hemi)cellulolytic Enzymes fromPleurotus ostreatus

An enzymatic mixture of cellulases and xylanases was produced byPleurotus ostreatususing microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis ofArundo donaxbioconversion. The Plackett-Burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreatedA. donax. As the most significant influence during the enzymatic hydrolysis ofA. donaxwas exercised by the temperature (°C), pH, and time, the combined effect of these factors in the bioconversion byP. ostreatuscellulase and xylanase was analyzed by a 33factorial experimental design. It is worth noting that the best result of 480.10 mg of sugars/gds, obtained at 45°C, pH 3.5, and 96 hours of incubation, was significant also when compared with the results previously reached by process optimization with commercial enzymes.

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Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

Scientific Reports ◽

10.1038/s41598-020-80719-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Chiranjeevi Thulluri ◽

Ravi Balasubramaniam ◽

Harshad Ravindra Velankar

Keyword(s):

Enzymatic Hydrolysis ◽

Rice Straw ◽

Deep Eutectic Solvent ◽

Solvent System ◽

Cyclic Ether ◽

Cellulolytic Enzymes ◽

Ammonium Bromide ◽

Selective Delignification ◽

Cellulose Iβ ◽

Hydrolysis Of

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.

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Evaluation of thermomechanical pretreatment for enzymatic hydrolysis of pure microcrystalline cellulose and cellulose from Brewers’ spent grain

Journal of Cereal Science ◽

10.1016/j.jcs.2011.06.004 ◽

2011 ◽

Vol 54 (3) ◽

pp. 305-310 ◽

Cited By ~ 16

Author(s):

Guillaume Pierre ◽

Frédéric Sannier ◽

Romain Goude ◽

Armelle Nouviaire ◽

Zoulikha Maache-Rezzoug ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Microcrystalline Cellulose ◽

Spent Grain ◽

Hydrolysis Of

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Determination of Opiates in Whole Blood and Vitreous Humor: A Study of the Matrix Effect and an Experimental Design to Optimize Conditions for the Enzymatic Hydrolysis of Glucuronides

Journal of Analytical Toxicology ◽

10.1093/jat/bks007 ◽

2012 ◽

Vol 36 (3) ◽

pp. 162-170 ◽

Cited By ~ 18

Author(s):

L. R. Sanches ◽

S. C. Seulin ◽

V. Leyton ◽

B. A. P. Bismara Paranhos ◽

C. A. Pasqualucci ◽

...

Keyword(s):

Experimental Design ◽

Enzymatic Hydrolysis ◽

Matrix Effect ◽

Whole Blood ◽

Vitreous Humor ◽

The Matrix ◽

Hydrolysis Of

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A new approach for modeling cellulase-cellulose adsorption and the kinetics of the enzymatic hydrolysis of microcrystalline cellulose

Biotechnology and Bioengineering ◽

10.1002/bit.260420410 ◽

1993 ◽

Vol 42 (4) ◽

pp. 469-479 ◽

Cited By ~ 60

Author(s):

Bernd Nidetzky ◽

Walter Steiner

Keyword(s):

Enzymatic Hydrolysis ◽

Microcrystalline Cellulose ◽

New Approach ◽

Kinetics Of ◽

Hydrolysis Of

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Effect of phosphoric acid pretreatment on enzymatic hydrolysis of microcrystalline cellulose

Biotechnology Advances ◽

10.1016/j.biotechadv.2010.05.010 ◽

2010 ◽

Vol 28 (5) ◽

pp. 613-619 ◽

Cited By ~ 43

Author(s):

Juanhua Zhang ◽

Beixiao Zhang ◽

Jingqiang Zhang ◽

Lu Lin ◽

Shijie Liu ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Phosphoric Acid ◽

Microcrystalline Cellulose ◽

Acid Pretreatment ◽

Hydrolysis Of

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Experimental design for enzymatic hydrolysis of Cistus ladanifer

Journal of Biotechnology ◽

10.1016/j.jbiotec.2008.07.587 ◽

2008 ◽

Vol 136 ◽

pp. S274-S275

Author(s):

S. Ferreira ◽

A.P. Duarte ◽

J.A. Queiroz ◽

F.C. Domingues

Keyword(s):

Experimental Design ◽

Enzymatic Hydrolysis ◽

Cistus Ladanifer ◽

Hydrolysis Of

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Physico-chemical properties of lignin fractions from acid pretreated corn stover and their effects on enzymatic hydrolysis of microcrystalline cellulose

BioResources ◽

10.15376/biores.15.3.4898-4911 ◽

2020 ◽

Vol 15 (3) ◽

pp. 4898-4911

Keyword(s):

Enzymatic Hydrolysis ◽

Physicochemical Properties ◽

Corn Stover ◽

Microcrystalline Cellulose ◽

Residual Lignin ◽

Surface Charges ◽

Pretreated Corn Stover ◽

Lignin Fraction ◽

Isolation Methods ◽

Hydrolysis Of

Lignin has been shown to be a recalcitrance factor in many biomass conversion studies. To better understand the effects of lignin on cellulose conversion, different lignin fractions were extracted from the same dilute acid pretreated corn stover by three sequential isolation methods, namely ethanol extraction, dioxane extraction, and enzyme purification. The physicochemical properties of each lignin fraction including molecular weight distribution, surface area, surface charge, and other structural features varied, depending on the isolation methods. All three lignin fractions had negative surface charges, and ethanol-extracted lignin carried the highest surface charges, followed by dioxane-extracted lignin and cellulase-purified residual lignin. These physicochemical properties of lignin fractions also resulted in different extent of inhibitory effects on enzymatic hydrolysis of microcrystalline cellulose (MCC). Dioxane-extracted lignin exhibited the highest inhibitory effect on glucose release from MCC, followed by the cellulase-purified residual lignin fraction and ethanol-extracted lignin. Furthermore, lignin fractions with higher contents of syringyl (S) substructure and β-O-4 aryl ether interunit linkages showed a stronger negative effect on cellulase hydrolysis of MCC.

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Effects of average molar weight, crystallinity, and hemicelluloses content on the enzymatic hydrolysis of sisal pulp, filter paper, and microcrystalline cellulose

Industrial Crops and Products ◽

10.1016/j.indcrop.2018.02.011 ◽

2018 ◽

Vol 115 ◽

pp. 280-289 ◽

Cited By ~ 15

Author(s):

Joice Jaqueline Kaschuk ◽

Elisabete Frollini

Keyword(s):

Enzymatic Hydrolysis ◽

Microcrystalline Cellulose ◽

Filter Paper ◽

Molar Weight ◽

Hydrolysis Of

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Study of Pretreatment and Enzymatic Hydrolysis on Microcrystalline Cellulose and HVS A4 Paper in Pressurized CO2 Media

Modern Applied Science ◽

10.5539/mas.v9n7p16 ◽

2015 ◽

Vol 9 (7) ◽

pp. 16 ◽

Cited By ~ 1

Author(s):

Ian Eka Pramudita ◽

Marcella Lauditta Noviana ◽

Henky Muljana

Keyword(s):

High Performance Liquid Chromatography ◽

Enzymatic Hydrolysis ◽

Microcrystalline Cellulose ◽

Levulinic Acid ◽

High Performance ◽

Model Compound ◽

Bioethanol Production ◽

Fixed Temperature ◽

Pre Treatment ◽

Hydrolysis Of

The aim of this work is to investigate the potential used of pressurized CO2 in the pre-treatment and the enzymatic hydrolysis of waste HVS A4 paperto produce a monomeric sugars (C5 and C6 sugars) which is a precursor for bioethanol production. Prior to the utilization of waste HVS A4 paper, the microcrystalline cellulose and HVS A4 paper were first used in the experiments as model compound in order to gain better insights of the process. The experiment consists of two main parts which are the preliminary experimentsto determine the best pretreatment conditionsbetween two selected pressure values (80 and 100 bar) at a fixed temperature (T = 75 oC) and the enzymatic hydrolysis experiments. In the latter, the microcrystalline and HVS A4 paper were hydrolyzed at different pressures (100 bar, 125 bar, and 150 bar) and at different cellulase intakes (1% (v/v), 3% (v/v), and 5% (v/v)) with a fixed temperature (50°C). The hydrolyzed products were analyzed with a High Performance Liquid Chromatography (HPLC) to quantify the monomeric sugars and to determine the presence of the side products (furfural, HMF and levulinic acid). Within the experimental range, a maximum glucose concentration of 7602.35 ppm and 4560.79 ppm are obtained for microcrystalline and HVS A4 paper, respectively. In addition, there are no furfural, HMF and levulinic acid detected in the products This study shows a potential used of pressurized CO2 in the pretreatment and enzymatic hydrolysis of the model compound and gives a better insight for further application.

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