Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model

Absorbable extracellular matrix (ECM) membrane has recently been used as a barrier membrane (BM) in guided tissue regeneration (GTR) and guided bone regeneration (GBR). Absorbable BMs are mostly based on collagen, which is more biocompatible than synthetic materials. However, implanted absorbable BMs can be rapidly degraded by enzymesin vivo. In a previous study, to delay degradation time, collagen fibers were treated with cross-linking agents. These compounds prevented the enzymatic degradation of BMs. However, cross-linked BMs can exhibit delayed tissue integration. In addition, the remaining cross-linker could induce inflammation. Here, we attempted to overcome these problems using a natural ECM membrane. The membrane consisted of freshly harvested porcine pericardium that was stripped from cells and immunoreagents by a cleaning process. Acellular porcine pericardium (APP) showed a bilayer structure with a smooth upper surface and a significantly coarser bottom layer. APP is an ECM with a thin layer (0.18–0.35 mm) but with excellent mechanical properties. Tensile strength of APP was14.15±2.24 MPa. Inin vivoexperiments, APP was transplanted into rabbit tibia. The biocompatible material was retained for up to 3 months without the need for cross-linking. Therefore, we conclude that APP could support osteogenesis as a BM for up to 3 months.

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Cross-Linking Methacrylated Porcine Pericardium by Radical Polymerization Confers Enhanced Extracellular Matrix Stability, Reduced Calcification, and Mitigated Immune Response to Bioprosthetic Heart Valves

ACS Biomaterials Science & Engineering ◽

10.1021/acsbiomaterials.9b00091 ◽

2019 ◽

Vol 5 (4) ◽

pp. 1822-1832 ◽

Cited By ~ 5

Author(s):

Linhe Jin ◽

Gaoyang Guo ◽

Wanyu Jin ◽

Yang Lei ◽

Yunbing Wang

Keyword(s):

Immune Response ◽

Extracellular Matrix ◽

Radical Polymerization ◽

Heart Valves ◽

Cross Linking ◽

Bioprosthetic Heart Valves ◽

Matrix Stability ◽

Porcine Pericardium

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Cell-assembled extracellular matrix (CAM): a human biopaper for the biofabrication of pre-vascularized tissues able to connect to the host circulation in vivo

Biofabrication ◽

10.1088/1758-5090/ac2f81 ◽

2021 ◽

Vol 14 (1) ◽

pp. 015005

Author(s):

H Oliveira ◽

C Médina ◽

G Labrunie ◽

N Dusserre ◽

S Catros ◽

...

Keyword(s):

Extracellular Matrix ◽

Vascular System ◽

Circulatory System ◽

Mice Model ◽

Tissue Integration ◽

Tissue Equivalent ◽

Human Material ◽

First Time

Abstract When considering regenerative approaches, the efficient creation of a functional vasculature, that can support the metabolic needs of bioengineered tissues, is essential for their survival after implantation. However, it is widely recognized that the post-implantation microenvironment of the engineered tissues is often hypoxic due to insufficient vascularization, resulting in ischemia injury and necrosis. This is one of the main limitations of current tissue engineering applications aiming at replacing significant tissue volumes. Here, we have explored the use of a new biomaterial, the cell-assembled extracellular matrix (CAM), as a biopaper to biofabricate a vascular system. CAM sheets are a unique, fully biological and fully human material that has already shown stable long-term implantation in humans. We demonstrated, for the first time, the use of this unprocessed human ECM as a microperforated biopaper. Using microvalve dispensing bioprinting, concentrated human endothelial cells (30 millions ml−1) were deposited in a controlled geometry in CAM sheets and cocultured with HSFs. Following multilayer assembly, thick ECM-based constructs fused and supported the survival and maturation of capillary-like structures for up to 26 d of culture. Following 3 weeks of subcutaneous implantation in a mice model, constructs showed limited degradative response and the pre-formed vasculature successfully connected with the host circulatory system to establish active perfusion.This mechanically resilient tissue equivalent has great potential for the creation of more complex implantable tissues, where rapid anastomosis is sine qua non for cell survival and efficient tissue integration.

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Study the Biodegradation of a Injection Human-Like Collagen Hydrogel

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.535-537.2291 ◽

2012 ◽

Vol 535-537 ◽

pp. 2291-2295 ◽

Cited By ~ 1

Author(s):

Yu Zhang Du ◽

Dai Di Fan ◽

Xiao Xuan Ma ◽

Chen Hui Zhu ◽

Li Jun Zhang

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

Carboxymethyl Chitosan ◽

Cross Linking ◽

In Vitro Degradation ◽

Collagen Hydrogel ◽

Degradation Time ◽

Scanning Electron

In this paper, the cross-linking hydrogels were synthesized by EDC crosslinker and Carboxymethyl chitosan (CMCS)/Human-like collagen (HLC). The morphology of the hydrogels was observed by scanning electron microscope (SEM). The important performance of these hydrogels is biodegradation which was assessed in two ways: (1) Through enzyme solution experiment in vitro , investigating in vitro degradation property. (2) Injecting the hydrogels to mice subcutaneous , after 2,4,12,and 24 weeks of healing ,the rats were sacrificed and explanted specimens were prepared for histology analysis. Investigating degradation time in vitro and in vivo , and studying the histology .HLC hydrogels degradation time gets eight to ten months, in addition has good compatibility of the histological performances. The hydrogels are promised for the applications in the biomaterials area.

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Desmopressin (DDAVP) Enhances Platelet Adhesion to the Extracellular Matrix of Cultured Human Endothelial Cells through Increased Expression of Tissue Factor

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656088 ◽

1997 ◽

Vol 77 (05) ◽

pp. 0975-0980 ◽

Cited By ~ 23

Author(s):

Angel Gálvez ◽

Goretti Gómez-Ortiz ◽

Maribel Díaz-Ricart ◽

Ginés Escolar ◽

Rogelio González-Sarmiento ◽

...

Keyword(s):

Extracellular Matrix ◽

Endothelial Cells ◽

Tissue Factor ◽

Platelet Adhesion ◽

Umbilical Vein ◽

Procoagulant Activity ◽

Experimental Conditions ◽

Chromogenic Assay

SummaryThe effect of desmopressin (DDAVP) on thrombogenicity, expression of tissue factor and procoagulant activity (PCA) of extracellular matrix (ECM) generated by human umbilical vein endothelial cells cultures (HUVEC), was studied under different experimental conditions. HUVEC were incubated with DDAVP (1, 5 and 30 ng/ml) and then detached from their ECM. The reactivity towards platelets of this ECM was tested in a perfusion system. Coverslips covered with DD A VP-treated ECMs were inserted in a parallel-plate chamber and exposed to normal blood anticoagulated with low molecular weight heparin (Fragmin®, 20 U/ml). Perfusions were run for 5 min at a shear rate of 800 s1. Deposition of platelets on ECMs was significantly increased with respect to control ECMs when DDAVP was used at 5 and 30 ng/ml (p <0.05 and p <0.01 respectively). The increase in platelet deposition was prevented by incubation of ECMs with an antibody against human tissue factor prior to perfusion. Immunofluorescence studies positively detected tissue factor antigen on DDAVP derived ECMs. A chromogenic assay performed under standardized conditions revealed a statistically significant increase in the procoagulant activity of the ECMs produced by ECs incubated with 30 ng/ml DDAVP (p <0.01 vs. control samples). Northern blot analysis revealed increased levels of tissue factor mRNA in extracts from ECs exposed to DDAVP. Our data indicate that DDAVP in vitro enhances platelet adhesion to the ECMs through increased expression of tissue factor. A similar increase in the expression of tissue factor might contribute to the in vivo hemostatic effect of DDAVP.

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