scholarly journals Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Carmela Nardelli ◽  
Laura Iaffaldano ◽  
Vincenzo Pilone ◽  
Giuseppe Labruna ◽  
Maddalena Ferrigno ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Gastric Banding ◽  
Laparoscopic Adjustable Gastric Banding ◽  
Subcutaneous Adipose Tissue ◽  
Adjustable Gastric Banding ◽  
Epigenetic Mechanism ◽  
Mirna Profile ◽  
Obese Patients ◽  
Subcutaneous Adipose

Background. Laparoscopic adjustable gastric banding (LAGB) results in significant lasting weight loss and improved metabolism in obese patients. To evaluate whether epigenetic factors could concur to these benefits, we investigated the subcutaneous adipose tissue (SAT) microRNA (miRNA) profile before (T0) and three years (T1) after LAGB in three morbidly obese women. Case Reports. SAT miRNA profiling, evaluated by TaqMan Array, showed four downexpressed (miR-519d, miR-299-5p, miR-212, and miR-671-3p) and two upexpressed (miR-370 and miR-487a) miRNAs at T1 versus T0. Bioinformatics predicted that these miRNAs regulate genes belonging to pathways associated with the cytoskeleton, inflammation, and metabolism. Western blot analysis showed that PPAR-alpha, which is the target gene of miR-519d, increased after LAGB, thereby suggesting an improvement in SAT lipid metabolism. Accordingly, the number and diameter of adipocytes were significantly higher and lower, respectively, at T1 versus T0. Bioinformatics predicted that the decreased levels of miR-212, miR-299-5p, and miR-671-3p at T1 concur in reducing SAT inflammation. Conclusion. We show that the miRNA profile changes after LAGB. This finding, although obtained in only three cases, suggests that this epigenetic mechanism, by regulating the expression of genes involved in inflammation and lipid metabolism, could concur to improve SAT functionality in postoperative obese patients.

Evaluate the effectiveness of hypoglycemia after laparoscopic bariatric gastric to treat diabete in non-insulin dependent patients due to obesity

2018 ◽  
Vol 8 (3) ◽  
Author(s):  
Thanh Phúc Bùi
Keyword(s):  
Gastric Banding ◽  
Laparoscopic Adjustable Gastric Banding ◽  
Adjustable Gastric Banding ◽  
Medical Condition ◽  
Control Group ◽  
Diabetic Patients ◽  
Viet Nam ◽  
Obese Patients ◽  
Insulin Dependent

Tóm tắt Đặt vấn đề: Béo phì là một tình trạng bệnh lý đang gia tăng tại Việt Nam. Phẫu thuật nội soi đặt vòng thắt dạ dày đã được ứng dụng trong điều trị bệnh béo phì. Trong số các người bệnh béo phì có không ít các người bệnh đái đường. Chúng tôi tiến hành thực hiên nghiên cứu này nhằm đánh giá hiệu quả giảm đường huyết sau phẫu thuật nội soi đặt vòng thắt dạ dày điều trị béo phì. Phương pháp nghiên cứu: Nghiên cứu mô tả tiến cứu không có nhóm chứng, theo dõi dọc Kết quả: 22 người bệnh đái tháo đường với BMI trung bình trước mổ là 39.9 được đánh giá đái tháo đường trước và sau mổ với 6 mức độ. Mức độ giảm đường huyết nhiều nhất tại thời điểm 3 tháng sau phẫu thuật. Kết luận: Phẫu thuật nội soi đặt vòng thắt dạ dày không chỉ làm giảm cân nặng mà còn giảm tỷ lệ đái tháo đường ở các người bệnh béo phì. Abstract Introduction: Obesity is a medical condition which has increased in Vietnam. Laparoscopic adjustable gastric banding has been used in the treatment of this disease in recent years. The study aims at evaluating the results of laparoscopic adjustable gastric banding in the treatment of Type 2 Diabetes Mellitus in Viet Duc Hospital. Material and Methods: Prospective descriptive study without a control group, vertical survery methods. Results: This retrospective study includes 22 obese diabetic patients with a preoperative BMI of 39.9± 7.8 kg/m2 who underwent gastric banding. A 6-point scoring system graded the level of anti-diabetic therapy. Downgrading reached its maximum at 3 months after operation. Conclusion: Laparoscopic adjustable gastric banding is a highly effective method to lose weight as well as decrease diabete rate in obese patients. Keyword: Diabetes, obesity, laparoscopic adjustable gastric banding.

Cefamandole Distribution in Serum, Adipose Tissue, and Wound Drainage in Morbidly Obese Patients

1986 ◽  
Vol 20 (11) ◽  
pp. 869-873 ◽  
Author(s):  
Henry J. Mann ◽  
Henry Buchwald
Keyword(s):  
Adipose Tissue ◽  
Rate Constant ◽  
Subcutaneous Adipose Tissue ◽  
Elimination Rate ◽  
Elimination Rate Constant ◽  
Morbidly Obese ◽  
Obese Patients ◽  
Tissue Concentrations ◽  
Wound Drainage ◽  
Subcutaneous Adipose

Distribution and elimination of cefamandole 2 g iv were studied in 11 morbidly obese patients during a gastric bypass operation and again on the first postoperative day. Serum, subcutaneous adipose tissue, wound drainage, and urine were analyzed by high performance liquid chromatography for cefamandole and pharmacokinetic parameters from the intraoperative period were compared to those obtained postoperatively. Total body clearance was significantly greater (p < 0.001) postoperatively (297 ml/min) than intraoperatively (254 ml/min). Volume changes were unpredictable but the elimination rate constant tended to increase postoperatively. Renal clearance and percentage of urinary recovery were significantly increased (p < 0.01) postoperatively. The patients had a mean (± SD) volume of the central compartment of 10.3 (± 2.3) L, volume at steady state of 18.3 (± 3.9) L, and elimination rate constant of 1.67 (± 0.63) h−1. Tissue concentrations of cefamandole were highest during the first hour after drug administration and were < 1 μg/g after 3.5 hours. Mean wound drainage concentrations ranged between 10 and 12 μg/ml during a dosing interval and dropped to 7 μg/ml 12 hours after the last dose. Intraoperative dosing of cefamandole is required to maintain subcutaneous adipose tissue concentrations > 1 μg/g during procedures longer than three hours in morbidly obese patients. A postoperative dose of cefamandole 2 g iv q6h will provide sustained and therapeutic concentrations in the wound drainage of morbidly obese patients.

Lipid metabolism and cellular features of skeletal muscle and subcutaneous adipose tissue in pigs differing in IGF-II genotype

2008 ◽  
Vol 34 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Delphine Gardan ◽  
Florence Gondret ◽  
Karijn Van den Maagdenberg ◽  
Nadine Buys ◽  
Stefaan De Smet ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Adipose
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