Anti-adipocyte scFv-Fc Antibody Suppresses Subcutaneous Adipose Tissue Development and Affects Lipid Metabolism in Minipigs

2009 ◽  
Vol 162 (3) ◽  
pp. 687-697 ◽  
Author(s):  
M. L. Cheng ◽  
S. M. Zhao ◽  
W. Z. Li ◽  
X. Zhang ◽  
C. R. Ge ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Subcutaneous Adipose Tissue ◽  
Tissue Development ◽  
Adipose Tissue Development ◽  
Subcutaneous Adipose

scholarly journals Distinct Patterns of PPARγ Promoter Usage, Lipid Degradation Activity, and Gene Expression in Subcutaneous Adipose Tissue of Lean and Obese Swine

2018 ◽  
Vol 19 (12) ◽  
pp. 3892 ◽  
Author(s):  
Bin Song ◽  
Shengwei Di ◽  
Shiquan Cui ◽  
Na Chen ◽  
Huan Wang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Subcutaneous Adipose Tissue ◽  
Growth Stages ◽  
Tissue Development ◽  
Fatty Acid Degradation ◽  
Acid Degradation ◽  
Mitochondrial Functions ◽  
Adipose Tissue Development ◽  
Subcutaneous Adipose

Subcutaneous adipose tissue is a loose connective tissue specializing in the regulation of energy storage and metabolization. In domesticated pigs (Sus scrofa), the temporal development of subcutaneous adipose tissue is critical for meat production. However, the regulation of adipose tissue development remains unclear. Here, the subcutaneous adipose tissue development was characterized and compared in lean (Danish-Landrace) and obese (Min) pigs at juvenile and the juvenile-to-adult growth stages. Using RNA sequencing, we profiled the transcriptome of subcutaneous adipose tissue isolated from 4- and 16-week-old pigs and identified 24,718 expressed transcription units. Of them, 6327 genes were differentially expressed between the breeds and/or developmental stages. Compared with obese pigs, upregulated genes in lean pigs showed significant function and pathway enrichment in fatty acid degradation and mitochondrial functions. Further analysis uncovered the distinct usage preferences of the three alternative peroxisome proliferator-activated receptor γ (PPARγ) promoters associated with the development of subcutaneous adipose tissue in both breeds. Transcriptome analysis of subcutaneous adipose tissue in lean and obese pigs suggested that marker-assisted selection of fatty acid degradation and PPARγ signaling pathways could be important directions for future pork quality improvement and modern breeding.

Measurement of subcutaneous adipose tissue development in children by the optical device LIPOMETER

2001 ◽  
Author(s):  
Reinhard Moeller ◽  
Renate Horejsi ◽  
Karl Sudi ◽  
Aloys Berg ◽  
Gilbert Reibnegger ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Tissue Development ◽  
Optical Device ◽  
Adipose Tissue Development ◽  
Subcutaneous Adipose

Lipid metabolism and cellular features of skeletal muscle and subcutaneous adipose tissue in pigs differing in IGF-II genotype

2008 ◽  
Vol 34 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Delphine Gardan ◽  
Florence Gondret ◽  
Karijn Van den Maagdenberg ◽  
Nadine Buys ◽  
Stefaan De Smet ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Adipose

scholarly journals Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Carmela Nardelli ◽  
Laura Iaffaldano ◽  
Vincenzo Pilone ◽  
Giuseppe Labruna ◽  
Maddalena Ferrigno ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Gastric Banding ◽  
Laparoscopic Adjustable Gastric Banding ◽  
Subcutaneous Adipose Tissue ◽  
Adjustable Gastric Banding ◽  
Epigenetic Mechanism ◽  
Mirna Profile ◽  
Obese Patients ◽  
Subcutaneous Adipose

Background. Laparoscopic adjustable gastric banding (LAGB) results in significant lasting weight loss and improved metabolism in obese patients. To evaluate whether epigenetic factors could concur to these benefits, we investigated the subcutaneous adipose tissue (SAT) microRNA (miRNA) profile before (T0) and three years (T1) after LAGB in three morbidly obese women. Case Reports. SAT miRNA profiling, evaluated by TaqMan Array, showed four downexpressed (miR-519d, miR-299-5p, miR-212, and miR-671-3p) and two upexpressed (miR-370 and miR-487a) miRNAs at T1 versus T0. Bioinformatics predicted that these miRNAs regulate genes belonging to pathways associated with the cytoskeleton, inflammation, and metabolism. Western blot analysis showed that PPAR-alpha, which is the target gene of miR-519d, increased after LAGB, thereby suggesting an improvement in SAT lipid metabolism. Accordingly, the number and diameter of adipocytes were significantly higher and lower, respectively, at T1 versus T0. Bioinformatics predicted that the decreased levels of miR-212, miR-299-5p, and miR-671-3p at T1 concur in reducing SAT inflammation. Conclusion. We show that the miRNA profile changes after LAGB. This finding, although obtained in only three cases, suggests that this epigenetic mechanism, by regulating the expression of genes involved in inflammation and lipid metabolism, could concur to improve SAT functionality in postoperative obese patients.

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