scholarly journals Isolation and Characterization of Thermophilic Bacteria from Jordanian Hot Springs: Bacillus licheniformis and Thermomonas hydrothermalis Isolates as Potential Producers of Thermostable Enzymes

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Balsam T. Mohammad ◽  
Hala I. Al Daghistani ◽  
Atef Jaouani ◽  
Saleh Abdel-Latif ◽  
Christian Kennes
Keyword(s):  
Bacillus Licheniformis ◽  
Hot Springs ◽  
Thermophilic Bacteria ◽  
Phenotypic Diversity ◽  
Thermostable Enzymes ◽  
Future Studies ◽  
Blast Search ◽  
Upgma Dendrogram ◽  
Isolation And Characterization

The aim of this study was the isolation and characterization of thermophilic bacteria from hot springs in Jordan. Ten isolates were characterized by morphological, microscopic, biochemical, molecular, and physiological characteristics. Sequencing of the 16S rDNA of the isolates followed by BLAST search revealed that nine strains could be identified as Bacillus licheniformis and one isolate as Thermomonas hydrothermalis. This is the first report on the isolation of Thermomonas species from Jordanian hot springs. The isolates showed an ability to produce some thermostable enzymes such as amylase, protease, cellulose, gelatins, and lecithin. Moreover, the UPGMA dendrogram of the enzymatic characteristics of the ten isolates was constructed; results indicated a high phenotypic diversity, which encourages future studies to explore further industrial and environmental applications.

scholarly journals Isolation and Characterization of Thermophilic Bacteria Indigenous to Al-Ahsa Desert

2020 ◽  
Vol 14 (3) ◽  
pp. 2157-2163
Author(s):  
Ahmed Anwar Al-Mulla ◽  
Ashraf Khalifa
Keyword(s):  
Standard Method ◽  
Thermophilic Bacteria ◽  
Soil Samples ◽  
Biotechnological Applications ◽  
Bacterial Isolates ◽  
Phenotypic Characteristics ◽  
Thermostable Enzymes ◽  
Biological Resources ◽  
Isolation And Characterization

Deciphering the biological resources across the Saudi niches is highly recommended for the prosperity. To this end, the aim of the current work was to isolate thermophilic bacteria from unexplored areas of Al-Ahsa region, and investigate their phenotypic characteristics. Three soil samples were collected from different desert sites of Al-Ahsa region. Thermophilic bacteria were isolated directly for soil samples into Thermus medium broth as a standard method. Single colonies of the actively growing bacterial isolates were preserved in 20% glycerol then kept at -80°C. The isolates were screened for production of thermostable enzymes using the commercially available kit API20E strip (bioMerieux, Marcy l’Etoile, France). Incubation were carried out at 50°C. It can be concluded that thermophilic bacteria in Al-Ahsa region harbor novel thermostable enzymes that might have biotechnological applications, in future.

scholarly journals Isolation and Characterization of Aerobic Thermophilic Bacteria from the Savusavu Hot Springs in Fiji

2008 ◽  
Vol 23 (4) ◽  
pp. 350-352 ◽  
Author(s):  
Vinay V. Narayan ◽  
Mohamed A. Hatha ◽  
Hugh W. Morgan ◽  
Dhana Rao
Keyword(s):  
Hot Springs ◽  
Thermophilic Bacteria ◽  
Isolation And Characterization

scholarly journals Screening and characterization of thermo-active enzymes of biotechnological interest produced by thermophilic Bacillus isolated from hot springs in Tunisia

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
Wajdi Thebti ◽  
Yosra Riahi ◽  
Rawand Gharsalli ◽  
Omrane Belhadj
Keyword(s):  
Thermal Water ◽  
Hot Springs ◽  
Thermophilic Bacteria ◽  
Effect Of Temperature ◽  
Thermostable Enzymes ◽  
Thermophilic Bacillus ◽  
Enzyme Substrates ◽  
First Time

As part of the contribution to the global efforts in research of thermostable enzymes being of industrial interest, we focus on the isolation of thermophilic bacteria from Tunisian hot springs. Among the collection of 161 strains of thermophilic Bacillus isolated from different samples of thermal water in Tunisia, 20% are capable of growing at 100°C and the rest grow at 70°C or above. Preliminary activity tests on media supplemented with enzyme-substrates confirmed that 35 strains produced amylases, 37 - proteases, 43 - cellulases, 31 - xylanases and 37 - mannanases. The study of the effect of temperature on enzyme activity led to determination of the optimal temperatures of activities that vary between 60 and 100°C. Several enzymes were active at high temperatures (80, 90 and 100°C) and kept their activity even at 110°C. Several isolated strains producing enzymes with high optimal temperatures of activity were described for the first time in this study. Both strains B62 and B120 are producers of amylase, protease, cellulase, xylanase, and mannanase. The sequencing of 16S DNA identified isolated strains as Geobacillus kaustophillus, Aeribacillus pallidus, Geobacillus galactosidasus and Geobacillus toebii.

scholarly journals Isolation and characterization of Anoxygenic phototrophs from shrimp ponds in Thailand

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Xelimar Ramirez ◽  
Imeleta Luamanu ◽  
Ruben Michael Ceballos ◽  
Elizabeth Padilla Crespo
Keyword(s):  
Environmental Samples ◽  
Production Systems ◽  
Phenotypic Diversity ◽  
Purple Bacteria ◽  
Shrimp Farming ◽  
Post Inoculation ◽  
Shrimp Ponds ◽  
Anoxygenic Phototrophs ◽  
Isolation And Characterization

Anoxygenic phototrophic purple bacteria are ubiquitous in aquatic and terrestrial environments and demonstrate broad phenotypic diversity. Purple bacteriaderive energy from light under anaerobic conditions via anoxygenic photosynthesis, a process in which water is not the electron donor. It has been suggested that these bacteria are useful for a variety of applications, including: wastewater treatment; heavy metal remediation; nitrogen fixation; and, control of CH4 emissions. In this study, the goal was to isolate and characterize PNSB from shrimp ponds in Thailand. Surface water and sediment were collected. Enrichment cultures were prepared using Pfenning’s mineral media. As indicated by development of reddish color and turbidity, anoxygenic phototrophic growth was observed within two days of incubation. Cultures in liquid media and on solid plates exhibited a deep red or purple color ten weeks post-inoculation. Under light microscopy, enrichments consist of communities dominated by thin, elongated gram-negative cells with granules of elemental sulfur, which are characteristic of purple bacteria. Molecular methods confirm the presence of pufLM, a genetic biomarker for purple bacteria (e.g., Thiohalocapsa marina, Allochromatium vinosum, Roseovarius tolerans). Initial sequencing of key genes (i.e., pufLM) indicate that these environmental samples contain novel isolates or “geographic variants” that have not been previously described. We have developed a few pure cultures of multiple species from these environmental samples. Since shrimp farming is a key industry in southern Thailand, the characterization of the microbial communities in these ecosystems, including anoxygenic phototrophs, will provide insights into how to maintain water quality in these food production systems.

scholarly journals Short Communication: The emergence and rise of indigenous thermophilic bacteria exploration from hot springs in Indonesia

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Kenny Lischer ◽  
ANANDA BAGUS RICHKY DIGDAYA PUTRA ◽  
Brian Wirawan Guslianto ◽  
Forbes Avila ◽  
Sarah Grace Sitorus ◽  
...  
Keyword(s):  
High Temperature ◽  
16S Rrna ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Short Communication ◽  
Hot Springs ◽  
Thermophilic Bacteria ◽  
Whole Genome ◽  
Thermostable Enzymes ◽  
The Pacific

Abstract. Lischer K, Putra ABRD, Guslianto BW, Avilla F, Sitorus SG, Nugraha Y, Sarmoko. 2020. Short Communication: The emergence and rise of indigenous thermophilic bacteria exploration from hot springs in Indonesia. Biodiversitas 21: 5474-5481. Indonesia is an archipelagic country located in the pacific ring of fire, and is estimated to cause numerous hot springs spread across the country. In addition, small living microbes have been explored in these locations since 1985. These microbes possess the ability to survive in areas with high temperature (more than 40oC-90oC), and are therefore termed thermophiles. Hence, massive explorations have been conducted on Java island and other unexplored areas at Sumatra to Papua in New Guinea islands. Moreover, a total of 71 hot springs characterized by the presence of thermophilic bacteria have been explored in Indonesia. These investigations ensue with various approaches, including through conventional and microbiological, 16S rRNA, as well as whole-genome sequencing methods. In addition to species exploration, the application of thermophiles has become a topic of interest from 1999, especially based on thermostable enzymes with the capacity to maintain activity at high-temperature conditions. These include amylase, protease, lipase, xylanase, esterase, and cellulase as the most common isolated form, which indicates the existence of significant extractable potentials. Hence, there is a need for further research in terms of both exploration and application purposes.

Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey

2009 ◽  
Vol 79 (3) ◽  
pp. 321-328 ◽  
Author(s):  
Ahmet Adiguzel ◽  
Hakan Ozkan ◽  
Ozlem Baris ◽  
Kadriye Inan ◽  
Medine Gulluce ◽  
...  
Keyword(s):  
Hot Springs ◽  
Thermophilic Bacteria ◽  
Identification And Characterization
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