Assessment of Striga gesnerioides (Willd.) Resistance and Genetic Characterization of Forty-Six Cowpea (Vigna unguiculata (L.) Walp.) Genotypes in Ghana

The parasitic weed, Striga gesnerioides, imposes physiological stress on cowpea (Vigna unguiculata (L.) Walp.) resulting in significant yield loss in the regions of northern Ghana. This warranted identification of resistant cowpeas for sustainable production. The current work aim was to identify Striga-resistant cowpea genotypes and assess their genetic relatedness. Forty-six (46) cowpea genotypes were screened in pots for their reaction to Striga samples obtained from the upper east, upper west, and northern regions of Ghana and validated with C42-2B and 61R-M2 markers involving DNA amplification by PCR assay. Sixteen polymorphic SSR primer pairs were used to assess genetic relatedness among 46 cowpea genotypes. Data were analyzed with PowerMarker V. 3.25 and a dendrogram was generated with MEGA 4. On the whole, 65.2% of the cowpea genotypes had stable resistance to S. gesnerioides from the regions of northern Ghana and 34.8% were susceptible. The C42-2B marker resolved as a single DNA band of 280 bp with segregation efficiency of 80% and 61R-M2 marker as double DNA bands of 320 bp and 380 bp with segregation efficiency of 60% associated with Striga resistance. Sixteen (16) polymorphic SSR primers distinguished all 46 cowpea genotypes into three clusters. Gene diversity ranged from 0.04 to 0.49 with an average of 0.29. The average allele frequency is 0.78, with a mean genetic diversity of 0.29. Polymorphism information content (PIC) varied from 0.08 to 1.00 with an average of 0.55. Therefore, cowpeas with Striga resistance and other desirable traits can be evaluated and released as varieties for farmers to cultivate.

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Genetic characterization of farmed and wild populations of African catfish (Clarias gariepinus Burchell, 1822) using the random amplified polymorphic marker

Journal of Agricultural Sciences Belgrade ◽

10.2298/jas2004375s ◽

2020 ◽

Vol 65 (4) ◽

pp. 375-389

Author(s):

Sa`adu Suleiman ◽

Mohammed Diyaware ◽

Mohammed Aliyu ◽

Zanna Mohammed

Keyword(s):

Genetic Relatedness ◽

Genetic Characterization ◽

Gene Diversity ◽

Clarias Gariepinus ◽

Polymorphic Marker ◽

African Catfish ◽

Wild Populations ◽

In The Wild ◽

The Mean

The genetic characterization of Clarias gariepinus was conducted in this study. Thirty (30) C. gariepinus specimens were collected, fifteen (15) each from the wild and farms in northeastern Nigeria for their genetic relatedness and diversity using the RAPD markers. DNA extraction from the blood sample was performed using the Gene Jet Genomic DNA Purification Kit. Five primers were used in employing PCR and amplified 402 RAPD bands from the four strains of Clarias gariepinus. Three hundred and six (76.12%) bands were polymorphic while 86 (21.39%) were monomorphic. The percentage of polymorphism obtained from farmed and wild populations ranged from 58 (47.3%) to 69 (75.9%), respectively. The polymorphic bands per loci within the populations ranged from 67.9% to 82.0%. The mean numbers of inbreeding coefficient (FIS) were 0.083 and 0.053 in the farmed and wild populations. Gene diversity values within farmed and wild populations (Ht) were 0.4522 and 0.4018. The mean genetic differentiation (FST) ranged between (0.203) in the farmed and 0.129 in the wild populations. The analysis of molecular variance revealed that there was 96% of genetic variance within the population and 4% among the population. The genetic identity and distance of four populations were 0.9490 and 0.1038, respectively. The phylogenetic measure has shown that the four strains were divided into two clusters at approximately 0.089 similarity levels. The result indicated a significant level of genetic variation and minimal dendrogram separation in Clarias gariepinus in northeastern Nigeria. Conclusively, this information will be a useful tool for the genetic and breeding program of Clarias gariepinus.

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Pyrolysis of the Caupi Bean Pod (Vigna unguiculata): Characterization of Biomass and Bio-Oil

Journal of the Brazilian Chemical Society ◽

10.21577/0103-5053.20190277 ◽

2020 ◽

Cited By ~ 1

Author(s):

Roberta Santos ◽

Diego Bispo ◽

Honnara Granja ◽

Eliana Sussuchi ◽

André Luis Ramos ◽

...

Keyword(s):

Vigna Unguiculata ◽

Bio Oil

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Diversity of Cowpea [Vigna unguiculata (L.) Walp] Landraces in Mozambique: New Opportunities for Crop Improvement and Future Breeding Programs

Agronomy ◽

10.3390/agronomy11050991 ◽

2021 ◽

Vol 11 (5) ◽

pp. 991

Author(s):

Ana Maria Figueira Gomes ◽

David Draper ◽

Nascimento Nhantumbo ◽

Rafael Massinga ◽

José C. Ramalho ◽

...

Keyword(s):

Vigna Unguiculata ◽

Production Systems ◽

Crop Improvement ◽

Morphological Diversity ◽

Ecological Zones ◽

Nutrition Security ◽

Food And Nutrition ◽

High Level ◽

Nutrition Sensitive

Cowpea (Vigna unguiculata) is a neglected crop native to Africa, with an outstanding potential to contribute to the major challenges in food and nutrition security, as well as in agricultural sustainability. Two major issues regarding cowpea research have been highlighted in recent years—the establishment of core collections and the characterization of landraces—as crucial to the implementation of environmentally resilient and nutrition-sensitive production systems. In this work, we have collected, mapped, and characterized the morphological attributes of 61 cowpea genotypes, from 10 landraces spanning across six agro-ecological zones and three provinces in Mozambique. Our results reveal that local landraces retain a high level of morphological diversity without a specific geographical pattern, suggesting the existence of gene flow. Nevertheless, accessions from one landrace, i.e., Maringué, seem to be the most promising in terms of yield and nutrition-related parameters, and could therefore be integrated into the ongoing conservation and breeding efforts in the region towards the production of elite varieties of cowpea.

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Characterization of Maize Genotypes (Zea mays L.) for Resistance to Striga asiatica and S. hermonthica and Compatibility with Fusarium oxysporum f. sp. strigae (FOS) in Tanzania

Agronomy ◽

10.3390/agronomy11051004 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1004

Author(s):

John Lobulu ◽

Hussein Shimelis ◽

Mark D. Laing ◽

Arnold Angelo Mushongi ◽

Admire Isaac Tichafa Shayanowako

Keyword(s):

Grain Yield ◽

Fusarium Oxysporum ◽

Biocontrol Agent ◽

Zea Mays L ◽

Control Treatment ◽

Yield Reduction ◽

Maize Genotypes ◽

Significant Yield ◽

The Mean

Striga species cause significant yield loss in maize varying from 20 to 100%. The aim of the present study was to screen and identify maize genotypes with partial resistance to S. hermonthica (Sh) and S. asiatica (Sa) and compatible with Fusarium oxysporum f. sp. strigae (FOS), a biocontrol agent. Fifty-six maize genotypes were evaluated for resistance to Sh and Sa, and FOS compatibility. Results showed that FOS treatment significantly (p < 0.001) enhanced Striga management compared to the untreated control under both Sh and Sa infestations. The mean grain yield was reduced by 19.13% in FOS-untreated genotypes compared with a loss of 13.94% in the same genotypes treated with FOS under Sh infestation. Likewise, under Sa infestation, FOS-treated genotypes had a mean grain yield reduction of 18% while untreated genotypes had a mean loss of 21.4% compared to the control treatment. Overall, based on Striga emergence count, Striga host damage rating, grain yield and FOS compatibility, under Sh and Sa infestations, 23 maize genotypes carrying farmer preferred traits were identified. The genotypes are useful genetic materials in the development of Striga-resistant cultivars in Tanzania and related agro-ecologies.

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Genetic diversity, variability and characterization of the agro-morphological traits of Northern Ghana Roselle (Hibiscus sabdariffa var. altissima) accessions

African Journal of Plant Science ◽

10.5897/ajps2019.1783 ◽

2019 ◽

Vol 13 (6) ◽

pp. 168-184

Author(s):

Y. Tetteh Antonia ◽

A. Ankrah Nii ◽

Coffie Nancy ◽

Niagiah Achana

Keyword(s):

Genetic Diversity ◽

Morphological Traits ◽

Northern Ghana ◽

Hibiscus Sabdariffa

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Extraction and characterization of starch from Yard-long bean (Vigna unguiculata (L.) Walp. ssp. unguiculata cv.-gr. sesquipedalis)

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2021.04.127 ◽

2021 ◽

Vol 181 ◽

pp. 1023-1029

Author(s):

Jiahao Li ◽

Shanying Zhang ◽

Zhen Zhang ◽

Saihao Ren ◽

Di Wang ◽

...

Keyword(s):

Vigna Unguiculata

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ISSR Marker Based Genetic Diversity in Morinda Spp. For Its Enhanced Collection, Conservation and Utilization

10.21203/rs.3.rs-666059/v1 ◽

2021 ◽

Author(s):

Lalit Arya ◽

Ramya Kossery Narayanan ◽

Anjali Kak ◽

Chitra Devi Pandey ◽

Manjusha Verma ◽

...

Keyword(s):

Genetic Diversity ◽

Gene Diversity ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Morinda Citrifolia ◽

Species Differentiation ◽

Information Index ◽

Upgma Cluster Analysis ◽

Simple Sequence

Abstract Morinda (Rubiaceae) is considerably recognized for its multiple uses viz. food, medicine, dyes, firewood, tools, oil, bio-sorbent etc. The molecular characterization of such an important plant would be very useful for its multifarious enhanced utilization. In the present study, 31 Morinda genotypes belonging to two different species Morinda citrifolia and Morinda tomentosa collected from different regions of India were investigated using Inter Simple Sequence Repeat (ISSR) markers. Fifteen ISSR primers generated 176 bands with an average of 11.7 bands per primer, of which (90.34%) were polymorphic. The percentage of polymorphic bands, mean Nei’s gene diversity, mean Shannon’s information index in Morinda tomentosa and Morinda citrifolia was [(69.89%, 30.68%); (0.21 ± 0.19, 0.12 ± 0.20); (0.32 ± 0.27 0.17 ± 0.28)] respectively, revealing higher polymorphism and genetic diversity in Morinda tomentosa compared to Morinda citrifolia. Structure, and UPGMA cluster analysis placed the genotypes into well-defined separate clusters belonging to two species Morinda tomentosa and Morinda citrifolia revealing the utility of ISSR markers in species differentiation. Distinct ecotypes within a particular species could also be inferred emphasizing the collection and conservation of Morinda genotypes from different regions, in order to capture the overall diversity of respective species. Further higher diversity of M. tomentosa must be advanced for its utilization in nutraceutical, nutritional and other nonfood purposes.

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