Effects of sEA on Slow Transit Constipation through the Microbiota-Gut-Brain Axis in Rats

To investigate the effect of sacral electroacupuncture (sEA) on the microbiota-gut-brain axis in the treatment of slow transit constipation, this study established a drug-induced model of slow transit constipation in rats and carried out sEA at the Baliao acupoints (BL31-BL34). On the 14th day of the therapeutic period (24 h fecal pellets), the aquaporin 3 (AQP3), 5-hydroxytryptamine (5-HT), and substance P (SP) transcripts from the distal colon and hypothalamus were analyzed. 16S rDNA has been widely used to analyze the diversity of the microbial communities. Therefore, in the present study, changes in the intestinal microbiota were analyzed by 16S rDNA gene sequencing. The results showed that sEA significantly increased the number of fecal pellets and the water content in the feces and reduced the reabsorption of intestinal water in 24 h. sEA also upregulated the level of SP mRNA expression in the distal colon and the hypothalamus, but downregulated the level of 5-HT mRNA expression in the distal colon. Moreover, sEA improved the Bacteroidetes to Firmicutes (B/F) ratio, which is beneficial to the general structure of the intestinal microflora. Our findings suggested that the microbiota-gut-brain axis constitutes a crucial pathological basis in the development of slow transit constipation. sEA improved the slow transit constipation by regulating the balance of the microbiota-gut-brain axis.

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mRNA expression of aquaporin protein 1 and 7 in rat model of slow transit constipation (STC)

African Journal of Pharmacy and Pharmacology ◽

10.5897/ajpp12.254 ◽

2012 ◽

Vol 6 (36) ◽

pp. 2643-2645 ◽

Cited By ~ 1

Author(s):

Zhen Li

Keyword(s):

Mrna Expression ◽

Rat Model ◽

Slow Transit Constipation ◽

Slow Transit ◽

Transit Constipation

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Decreased TRK C may reflect developmental abnormalities in Hirschsprung's disease and idiopathic slow transit constipation

Gastroenterology ◽

10.1016/s0016-5085(01)80111-6 ◽

2001 ◽

Vol 120 (5) ◽

pp. A23-A23

Author(s):

P FACER ◽

C KNOWLES ◽

P THOMAS ◽

P TAM ◽

N WILLIAMS ◽

...

Keyword(s):

Hirschsprung's Disease ◽

Hirschsprung’S Disease ◽

Slow Transit Constipation ◽

Developmental Abnormalities ◽

Slow Transit ◽

Transit Constipation

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Autoimmune encephalitis and gastrointestinal dysmotility: achalasia, gastroparesis, and slow transit constipation

Zeitschrift für Gastroenterologie ◽

10.1055/a-1233-2190 ◽

2020 ◽

Vol 58 (10) ◽

pp. 975-981

Author(s):

Thomas Frieling ◽

Christian Kreysel ◽

Michael Blank ◽

Dorothee Müller ◽

Ilka Melchior ◽

...

Keyword(s):

Interstitial Cells Of Cajal ◽

Autoimmune Encephalitis ◽

Enteric Neurons ◽

Slow Transit Constipation ◽

Gastrointestinal Dysmotility ◽

Slow Transit ◽

Small Intestinal ◽

Interdigestive Motility ◽

Cells Of Cajal ◽

Transit Constipation

Abstract Background Neurological autoimmune disorders (NAD) are caused by autoimmune inflammation triggered by specific antibody subtypes. NAD may disturb the gut-brain axis at several levels including brain, spinal cord, peripheral, or enteric nervous system. Case report We present a case with antinuclear neuronal Hu (ANNA-1)- and antiglial nuclear (SOX-1) autoimmune antibody-positive limbic encephalitis and significant gastrointestinal dysmotility consisting of achalasia type II, gastroparesis, altered small intestinal interdigestive motility, and severe slow transit constipation. The autoantibodies of the patient’s serum labeled enteric neurons and interstitial cells of Cajal but no other cells in the gut wall. Achalasia was treated successfully by pneumatic cardia dilation and gastrointestinal dysmotility successfully with prucalopride. Conclusion NAD may disturb gastrointestinal motility by altering various levels of the gut-brain axis.

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Prevalence and Clinical Characteristics of Dyssynergic Defecation and Slow Transit Constipation in Patients with Chronic Constipation

Journal of Clinical Medicine ◽

10.3390/jcm10092027 ◽

2021 ◽

Vol 10 (9) ◽

pp. 2027

Author(s):

Samuel Tanner ◽

Ahson Chaudhry ◽

Navneet Goraya ◽

Rohan Badlani ◽

Asad Jehangir ◽

...

Keyword(s):

Medical History ◽

Chronic Constipation ◽

Clinical Characteristics ◽

Anorectal Function ◽

Slow Transit Constipation ◽

Dyssynergic Defecation ◽

Patient Demographics ◽

Balloon Expulsion ◽

Slow Transit ◽

Transit Constipation

Patients with chronic constipation who do not respond to initial treatments often need further evaluation for dyssynergic defecation (DD) and slow transit constipation (STC). The aims of this study are to characterize the prevalence of DD and STC in patients referred to a motility center with chronic constipation and correlate diagnoses of DD and STC to patient demographics, medical history, and symptoms. High-resolution ARM (HR-ARM), balloon expulsion testing (BET) and whole gut transit scintigraphy (WGTS) of consecutive patients with chronic constipation were reviewed. Patients completed questionnaires describing their medical history and symptoms at the time of testing. A total of 230 patients completed HR-ARM, BET, and WGTS. Fifty (22%) patients had DD, and 127 (55%) patients had STC. Thirty patients (13%) had both DD and STC. There were no symptoms that were suggestive of STC vs. DD; however, patients with STC and DD reported more severe constipation than patients with normal transit and anorectal function. Patients with chronic constipation often need evaluation for both DD and STC to better understand their pathophysiology of symptoms and help direct treatment.

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