Simultaneous Determination of Phenolic Acids, Anthraquinones, Flavonoids, and Triterpenes of Cynomorii Herba in Different Harvest Times by LC-MS/MS

A sensitive and accurate method was developed for the simultaneous determination of twelve components including phenolic acids (gallic acid, protocatechuic aldehyde, protocatechuic acid, and ferulic acid), flavonoids (catechin, epicatechin, rutin, luteolin, luteolin-7-glucoside, and epicatechin gallate), anthraquinones (emodin), and triterpenes (ursolic acid) in Cynomorii herba in different harvest times by liquid chromatography tandem mass spectrometry (LC-MS/MS). The chromatographic separation was achieved on an Eclipse plus C18 (3.0 mm × 50 mm, 1.8 μm) column at 40°C. The mobile phase consisted of acetonitrile and 0.05% formic acid with a gradient elution at a flow rate of 0.4 mL·min −1. Under the optimized conditions, there was good linear relation (r ≥ 0.9944) and satisfactory precision (RSD values less than 5.65%). The recoveries of the twelve components were in the range of 93.5–105%. Moreover, the limits of detection (LOD) ranged from 0.003 to 21 ng mL−1 for the twelve analytes. In conclusion, the validated method was successfully applied to analyze the change regularity of the twelve components of Cynomorii herba in different harvest times. It provides a theoretical basis for choosing the suitable harvesting time of Cynomorii herba.

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A trace analysis method based on HPLC-MS/MS for the simultaneous determination of residues of the two isomers of pyrisoxazole in tomato fruit and soil

Analytical Methods ◽

10.1039/c5ay03358f ◽

2016 ◽

Vol 8 (9) ◽

pp. 2041-2047 ◽

Cited By ~ 5

Author(s):

He Zhu ◽

Fuyan Jia ◽

Qi Ding ◽

Chengtian Huang ◽

Yang Yu ◽

...

Keyword(s):

Simultaneous Determination ◽

High Performance ◽

Tomato Fruit ◽

Accurate Method ◽

Tandem Mass ◽

Analysis Method ◽

Soil P ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass

A simple, sensitive and accurate method for the simultaneous determination of residues of the two isomers of pyrisoxazole in tomato fruit and soil was established using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).

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Simultaneous Determination of Six Uncaria Alkaloids in Mouse Blood by UPLC–MS/MS and Its Application in Pharmacokinetics and Bioavailability

BioMed Research International ◽

10.1155/2020/1030269 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Lianguo Chen ◽

Jianshe Ma ◽

Xianqin Wang ◽

Meiling Zhang

Keyword(s):

Simultaneous Determination ◽

Oral Absorption ◽

Matrix Effects ◽

Multiple Reaction Monitoring ◽

Internal Standard ◽

Gradient Elution ◽

Mouse Blood ◽

The Matrix ◽

Liquid Chromatography Tandem Mass

A specific ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the simultaneous determination of six Uncaria alkaloids in mouse blood with midazolam as the internal standard (IS). Only 20 μL blood was needed for sample preparation, and the protein was precipitated with acetonitrile. The UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm) was used for chromatographic separation. The mobile phase consisted of 0.1% formic acid and acetonitrile with gradient elution within 5.5 min. Multiple reaction monitoring (MRM) and the positive electrospray ionization model were used for quantitative analysis. The accuracy of the UPLC-MS/MS method ranged from 86.5% to 110.4%. The precision for intraday and interday was ≤15% each. The mean recovery and the matrix effects were found to be 64.4-86.8% and 94.1-109.4%, respectively. The calibration curves in blood were linear in the range of 1-1000 ng/mL with a favorable correlation coefficient (r2) of 0.995. The pharmacokinetic results showed that six Uncaria alkaloids metabolized rapidly in mice with a half-life between 0.6 h and 4.4 h. The bioavailability of corynoxeine, isocorynoxeine, rhynchophylline, isorhynchophylline, hirsutine, and hirsuteine was 27.3%, 32.7%, 49.4%, 29.5%, 68.9%, and 51.0%, respectively, which showed satisfactory oral absorption of each alkaloid.

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Simultaneous Determination of Quercitrin, Afzelin, Amentoflavone, Hinokiflavone in Rat Plasma by UFLC–MS-MS and Its Application to the Pharmacokinetics of Platycladus orientalis Leaves Extract

Journal of Chromatographic Science ◽

10.1093/chromsci/bmy066 ◽

2018 ◽

Vol 56 (10) ◽

pp. 895-902 ◽

Cited By ~ 6

Author(s):

Chen-xiao Shan ◽

Shu-chen Guo ◽

Sheng Yu ◽

Ming-qiu Shan ◽

Sam Fong Yau Li ◽

...

Keyword(s):

Simultaneous Determination ◽

Pharmacokinetic Study ◽

Internal Standard ◽

Gradient Elution ◽

Rat Plasma ◽

Platycladus Orientalis ◽

Chromatography Tandem Mass Spectrometry ◽

Relative Standard ◽

Liquid Chromatography Tandem Mass

Abstract Leaves of Platycladus orientalis have been used as blood cooling and homeostatic therapy for thousands of years in traditional Chinese medicine. Emerging evidences of modern pharmacology have proved flavonoids as the key elements responsible for the efficacies. However, there has been no report on pharmacokinetic study of the flavonoids from Platycladus orientalis leaves extract. In this study, a sensitive and rapid ultra-flow liquid chromatography-tandem mass spectrometry method was established and validated for the simultaneous determination of amentoflavone, afzelin, hinokiflavone and quercitrin in rat plasma. The four flavonoids and luteolin (internal standard, IS) were recovered from rat plasma by methanol–ethyl acetate (v:v, 50:50). Chromatographic separation was performed on a C18 column with gradient elution. Our results showed that the recoveries from spiked control samples were more than 85% for all analytes and IS. The relative standard deviations of intra-day and inter-day precision were within 15% while the REs ranged from −6.6% to 8.0%. The validated method in this study was successfully applied to pharmacokinetic study in healthy rats after oral administration of P. orientalis leaves extract.

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Simultaneous Determination of 11 Compounds in Gualou Guizhi Granule and Pharmacokinetics Study by UPLC-MS/MS

Journal of Analytical Methods in Chemistry ◽

10.1155/2017/8451383 ◽

2017 ◽

Vol 2017 ◽

pp. 1-13 ◽

Cited By ~ 3

Author(s):

Chengtao Sun ◽

Wen Xu ◽

Yuqin Zhang ◽

Lishuang Yu ◽

Miao Ye ◽

...

Keyword(s):

Simultaneous Determination ◽

Gradient Elution ◽

Rat Plasma ◽

Negative Ion ◽

Detection Range ◽

Pharmacokinetics Study ◽

The Matrix ◽

Liquid Chromatography Tandem Mass ◽

Negative Ion Mode

A rapid and sensitive ultrafast performance liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS) was developed for the simultaneous determination of 11 compounds in Gualou Guizhi Granule (GLGZG), including liquiritin, isoliquiritin, liquirtin apioside, isoliquiritin apioside, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, glycyrrhetinic acid, paeoniflorin, albiflorin, and paeoniflorin sulfonate in rat plasma. UPLC-MS/MS assay with negative ion mode was performed on a Waters CORTECS C18 (2.1 × 100 mm, 1.6 μm) with the mobile phase consisting of 0.1% aqueous formic acid (A) and acetonitrile (B) in gradient elution at a flow rate of 0.25 mL·min−1. The method was linear for all analytes within the detection range (r≥0.9597). The inter- and intraday precision (RSD) were 2.21–6.41% and 1.67–6.18%; the inter- and intraday accuracy (recover) were 92.48–114.03% and 90.23–112.04%. And the recovery rate ranged from 81.30% to 108.22%. The matrix effect values obtained for analytes ranged from 88.91% to 113.32%. This validated method was successfully applied to a pharmacokinetics study in rats after oral administration of GLGZG.

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Simultaneous determination of pyrrolizidine alkaloids in food supplements of herbal origin using liquid chromatography tandem mass spectrometry

Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam ◽

10.47866/2615-9252/vjfc.58 ◽

2019 ◽

Vol 2 (1) ◽

pp. 24-31

Author(s):

Son Tran Cao ◽

Tien Bui Cao ◽

Thao Dang Phuong ◽

Truong Nguyen Xuan ◽

To Quynh Cung Thi ◽

...

Keyword(s):

Simultaneous Determination ◽

Pyrrolizidine Alkaloids ◽

Accurate Method ◽

Food Supplement ◽

Plant Origin ◽

Performance Requirement ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass ◽

Reactive Monitoring

A simple and accurate method has been developed for the simultaneous determination of four pyrrolizidine alkaloids (PAs) including echimidine, senecionine, jacobine and intermidine in food supplements of plant origin. The instrumental conditions including the use of TSK-Gel column for the separation and an MS/MS detector in positive ESI source, multi-reactive monitoring (MRM) mode for quantitation were optimized. The method has fully validated and meets the performance requirement of AOAC with good specificity. The linearity varies from 1.0 µg/kg to 40 µg/kg, the LOD was of 0.3 µg/kg; the repeatability and recovery range from 3.6% to 7.5% and 88.7% to 117%, respectively. The method was applied to determine PAs in 90 food supplement samples, of which 26 samples (28.8%) contain PAs.

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