Comparison between Cryopreserved and Dehydrated Human Amniotic Membrane Graft in Treating Challenging Cases with Macular Hole and Macular Hole Retinal Detachment

Purpose. To evaluate the surgical outcomes of cryopreserved and dehydrated human amniotic membrane (hAM) graft transplantation for macular hole (MH) and macular hole retinal detachment (MHRD) repair. Materials and Methods. This retrospective, interventional case series was conducted in two hospitals. Two types of hAM grafts, namely, the dehydrated form (AmnioGen, HCT Regenerative, Taiwan) and the cryopreserved form (AmnioGraft, Bio-Tissue, Miami, FL), were consecutively used in MH surgeries. Anatomical and functional outcomes between the 2 types of hAM grafts were compared. Results. Seventeen patients (mean age: 62.1 ± 10.0 years, 9 (52.9%) males) were enrolled. Of them, 11 patients had persistent MH, 3 had MH without prior surgery, and 3 had MHRD. A cryopreserved hAM graft was used in 10 patients, and a dehydrated hAM graft was used in 8 patients. One patient used a cryopreserved hAM in the first MH surgery and a dehydrated hAM in the second surgery for extramacular hole with retinal detachment. After a 6-month follow-up, 13 (76.5%) patients had sealed MHs. The average visual acuity (VA) of cases with sealed MHs improved from 1.38 ± 0.62 to 1.12 ± 0.47 logMAR (p=0.03). In the other 4 cases with persistent MH, 3 had graft dislocation and 1 had a reopened MH with graft contraction. There were no significant differences in closure rate (80.00% vs. 71.43%, p=0.68) or VA improvement (0.19 ± 0.37 logMAR vs. 0.15 ± 0.41 logMAR, p=0.85) between the 2 kinds of hAM graft. Conclusion. This preliminary case series showed that both cryopreserved hAM and dehydrated hAM are feasible alternative grafts for either persistent or recurrent MH. Both approaches have similar anatomical and functional outcomes.

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Novel method of plugging the hole: Anatomical and functional outcomes of human amniotic membrane-assisted macular hole surgery

Journal of Current Ophthalmology ◽

10.4103/joco.joco_189_20 ◽

2020 ◽

Vol 32 (4) ◽

pp. 361

Author(s):

Ramesh Venkatesh ◽

NareshKumar Yadav ◽

Sherina Thomas ◽

Arpitha Pereira ◽

KBhujang Shetty

Keyword(s):

Macular Hole ◽

Amniotic Membrane ◽

Functional Outcomes ◽

Human Amniotic Membrane ◽

Macular Hole Surgery ◽

Novel Method

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Silicone Oil Reinjection without Macular Buckling for Treatment of Recurrent Myopic Macular Hole Retinal Detachment after Silicone Oil Removal

Journal of Ophthalmology ◽

10.1155/2014/434272 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Hammouda Hamdy Ghoraba ◽

Sameh Mohamed Elgouhary ◽

Hosam Osman Mansour

Keyword(s):

Retinal Detachment ◽

Macular Hole ◽

Silicone Oil ◽

Case Series ◽

Oil Removal ◽

Interventional Study ◽

Silicone Oil Removal ◽

Macular Hole Retinal Detachment ◽

Medical Reports ◽

Myopic Macular Hole

Purpose. To evaluate the efficacy of silicone oil (S.O) reinjection without macular buckling for treatment of recurrent myopic macular hole retinal detachment (MHRD) after silicone oil removal.Methods. A retrospective consecutive interventional study from medical reports on cases of myopic MHRD. Fifty-three eyes of 51 patients underwent silicone oil removal after successful repair of MHRD were reviewed. The main outcomes were the retinal status after silicone oil removal and management of recurrent cases.Results. The rate of recurrent RD (Re RD) after silicone oil removal was 11.3% (6 out of 53 eyes). One case refused any other interference. In the remaining 5 eyes, 4 eyes (80%) could be reattached by S.O re-injection and one eye (20%) developed Re RD after S.O re-injection. Range of followup after management of recurrence was 5–53 months (mean 18.7 months).Conclusions. This case series concluded that the risk factors for recurrent RD after silicone oil removal from cases of myopic MHRD were high myopia, open flat MH, and large posterior staphyloma. Revision of vitrectomy and S.O re-injection can reattach most of recurrent cases.

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A Comparative Study between Vitrectomy with Internal Tamponade and a New Modified Fiber Optic Illuminated Ando Plombe for Cases of Macular Hole Retinal Detachment in Myopic Eyes

Journal of Ophthalmology ◽

10.1155/2015/841925 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Ahmed M. Bedda ◽

Ahmed M. Abdel Hadi ◽

Muhammad S. Abd Al Shafy

Keyword(s):

Retinal Detachment ◽

Success Rate ◽

Macular Hole ◽

Fiber Optic ◽

Case Series ◽

Successful Outcome ◽

Significant Difference ◽

Macular Hole Retinal Detachment ◽

Pars Plana ◽

Light Group

Aim.To compare pars plana vitrectomy (PPV) with silicone tamponade or gas (Groups Ia and Ib) and a new modified Ando plombe equipped with a fiber optic light (Group II) for cases with macular hole retinal detachment (MHRD) in high myopic eyes (axial length > 26 mm).Methods.A prospective interventional randomized case series included 60 eyes (20 in each group). Successful outcome was considered if the retina was completely attached at the end of the follow-up period. Complications were identified for each group.Results.Visual acuity improved by 37.31%, 40.67%, and 49.40% in Groups Ia, Ib, and II, respectively. The success rate was 55%, 60%, and 100% in Groups Ia , Ib, and II, respectively, with a statistically significant difference between Groups Ia, Ib, and II (p< 0.001 in Ia,p: 0.002 in Ib). Complications rates were 60%, 45%, and 20% in Groups Ia, Ib, and II, respectively, with a statistically significant difference between Groups Ia and II (p: 0.01).Conclusion.Fiber optic illuminated Ando plombe allows better positioning under the macula and consequently improves the success rate of epimacular buckling in comparison to PPV with internal tamponade in MMHRD.

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Parafoveal atrophy after human amniotic membrane graft for macular hole in patients with high myopia

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2019-315603 ◽

2020 ◽

pp. bjophthalmol-2019-315603

Author(s):

Der-Chong Tsai ◽

Yu-Hsuan Huang ◽

Shih-Jen Chen

Keyword(s):

Macular Hole ◽

Amniotic Membrane ◽

Axial Length ◽

High Myopia ◽

Rare Complication ◽

Case Series ◽

Visual Outcome ◽

Human Amniotic Membrane ◽

Significant Difference

PurposeTo report the surgical outcome and postoperative hypopigmented change around fovea among patients with high myopia who received human amniotic membrane (hAM) graft transplantation for macular hole (MH).MethodThis retrospective, interventional case series included 10 eyes of 10 consecutive patients (5 (50%) male) with high myopia (axial length over 26.5 mm) who received hAM graft to treat persisted or chronic MH with or without retinal detachment in two hospitals. Postoperative parafoveal atrophy was identified with colour fundus picture and structure optical coherent tomography. Baseline characteristics and short-term visual outcome were analysed.ResultsThe preoperative mean (±SD) axial length and MH diameter were 29.9 (±1.8) mm and 881.8 (±438.5) μm, respectively. After hAM transplantation, seven (70%) eyes had complete MH closure and the mean best-corrected visual acuity (BCVA) improved from 1.26 (±0.48) logarithm of minimal angle of resolution (logMAR) before operation to 1.11 (±0.44) logMAR on the last visit (p=0.074). Patchy atrophy-like depigmentation developed around the MH lesion in four (40.0%) eyes as early as in the first month after surgery. None of them had visual worsening. In terms of demographics, axial length, MH size, ocular history, preoperative BCVA and postoperative BCVA, there was no significant difference between those with and without the parafoveal atrophy. No graft rejection and inflammation happened during the follow-up.ConclusionParafovea atrophy, a rare complication in the conventional MH surgery, was observed in 40% of eyes with highly myopic MH after hAM graft transplantation. The pathogenesis and long-term consequence need further investigations.

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Next-Generation Sequencing-Based Molecular Diagnosis of Choroideremia

Case Reports in Ophthalmology ◽

10.1159/000437348 ◽

2015 ◽

Vol 6 (2) ◽

pp. 246-250 ◽

Cited By ~ 4

Author(s):

Kayo Shimizu ◽

Akio Oishi ◽

Maho Oishi ◽

Ken Ogino ◽

Satoshi Morooka ◽

...

Keyword(s):

Next Generation Sequencing ◽

Retinal Detachment ◽

Macular Hole ◽

Molecular Diagnosis ◽

Novel Mutation ◽

Case Series ◽

Next Generation ◽

Macular Hole Retinal Detachment ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing

We screened patients with choroideremia using next-generation sequencing (NGS) and identified a novel mutation and a known mutation in the CHM gene. One patient presented an atypical fundus appearance for choroideremia. Another patient presented macular hole retinal detachment in the left eye. The present case series shows the utility of NGS-based screening in patients with choroideremia. In addition, the presence of macular hole in 1 of the 2 patients, together with a previous report, indicated the susceptibility of patients with choroideremia to macular hole.

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Human amniotic membrane grafts for retinal breaks in diabetic tractional retinal detachment and combined tractional and rhegmatogenous retinal detachment

Scientific Reports ◽

10.1038/s41598-021-86804-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yen-Chih Chen ◽

San-Ni Chen

Keyword(s):

Retinal Detachment ◽

Tissue Regeneration ◽

Amniotic Membrane ◽

Rhegmatogenous Retinal Detachment ◽

Case Series ◽

Control Group ◽

Human Amniotic Membrane ◽

Ellipsoid Zone ◽

Retinal Breaks ◽

Additional Surgery

AbstractTo describe the surgical outcomes of using human amniotic membrane (hAM) grafts in the management of retinal breaks in diabetic tractional detachment (TRD) and combined tractional and rhegmatogenous retinal detachment (CTRRD). A retrospective case series of 10 eyes with TRD or CTRRD receiving pars plana vitrectomy with hAM grafts implantation, compared with 13 controls receiving the same surgery without hAM grafts. Best-corrected visual acuity (BCVA) and re-detachment rate were compared between two groups. Postoperatively, all eyes in the hAM group had retina attachment without recurrence, while 9 eyes in the control group had retina re-detachment and required additional surgery (0% vs 69.2%, p = 0.003). The BCVA significantly improved in the hAM group (from 1.96 ± 0.95 to 1.44 ± 0.77 in log MAR, p = 0.03), but not improved in control group (p = 0.20). Postoperative optical coherence tomography of the eyes receiving hAM grafts demonstrated glial tissue regeneration and restoration of ellipsoid zone. In diabetic TRD or CTRRD, hAM grafts could be an effective method, with promising outcome. Compared to standard surgery, it could result in higher retina reattachment rate and significant visual improvement. Moreover, it may offer the adjunctive benefit in tissue regeneration and fasten ellipsoid zone restoration.

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Exhibiting Residual Subretinal Fluid after High Myopic Macular Hole Retinal Detachment Surgery

Journal of the Korean Ophthalmological Society ◽

10.3341/jkos.2021.62.12.1672 ◽

2021 ◽

Vol 62 (12) ◽

pp. 1672-1678

Author(s):

Sung Joon Kim ◽

Jae Jung Lee ◽

Ik Soo Byon ◽

Ji Eun Lee ◽

Sung Who Park

Keyword(s):

Retinal Detachment ◽

Macular Hole ◽

Silicone Oil ◽

Subretinal Fluid ◽

Internal Limiting Membrane ◽

Closure Rate ◽

Visual Prognosis ◽

Membrane Peeling ◽

Macular Hole Retinal Detachment ◽

The Right

Purpose: To report a case of poor visual prognosis complicated by residual subretinal fluid after use of the internal limiting membrane flap technique to treat macular hole retinal detachment in a patient with high myopia.Case summary: A 55-year-old male stated that he had experienced a transparent circle in the central visual field of the right eye for 1 month. His best-corrected visual acuity (BCVA) was 0.32 and the axial length of the right eye was 32.57 mm. Fundus examination revealed a macular hole with retinal detachment localized to the posterior pole. We performed vitrectomy, membrane peeling, internal limiting membrane peeling with inverted internal limiting membrane flap, and silicone oil injection. On day 1 after surgery, the macular hole was closed, but subretinal fluid was noticed in the macula. At 3 months after surgery, the BCVA was 0.16 and the silicone oil was removed. At 14 months after the first surgery, the subretinal fluid was completely absorbed, but leopard-pattern pigment degeneration became prominent and the macula exhibited ellipsoid zone disruption. The BCVA decreased to 0.1.Conclusions: In patients exhibiting macular hole retinal detachment in the context of high myopia, an inverted internal limiting membrane flap may increase the macular hole closure rate but disturb subretinal fluid drainage. As persistent subretinal fluid may cause permanent retinal damage with a poor visual prognosis, the surgical method must be carefully chosen.

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Lyophilized amniotic membrane patch (LAMPatch) as a replacement of tamponades in the treatment of primary rhegmatogenous retinal detachment

International Journal of Retina and Vitreous ◽

10.1186/s40942-020-00264-7 ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Mario Saravia ◽

Luis Zeman ◽

Alejandro Berra

Keyword(s):

Intraocular Pressure ◽

Retinal Detachment ◽

Amniotic Membrane ◽

Case Series ◽

Pressure Rise ◽

Human Amniotic Membrane ◽

Retinal Breaks ◽

23 Gauge ◽

Membrane Patch

Abstract Background The basis of retinal detachment repair is sealing the retinal breaks. In order to seal the retinal breaks, chorioretinal adhesion around these lesions has to be achieved. Laser retinopexy is not immediate thus necessitates the use of a temporal endotamponade to maintain both tissues in apposition. We propose the use of a patch of lyophilized human amniotic membrane (LAMPatch) in order to occlude the retinal tear effectively until the chorioretinal adhesion is settled, overcoming the risks and limitations of the current tamponades. Methods 23-gauge vitrectomy was performed on eyes with primary retinal detachment with single retinal breaks of less than one-hour extension. A LAMPatch was deployed over the retinal breaks after retina was repositioned with perfluorocarbon. Neither gas nor silicon oil were injected. Results Six eyes of six patients with total or partial retinal detachment were included. Retinas remained reattached in all cases until the end on follow-up (3, 5 months). Best-corrected visual acuity at 1-week postop was between 20/30 and 20/100. Neither elevations of intraocular pressure, cataracts nor signs of inflammation were registered during follow-up. No second surgeries were needed. Conclusion This technique has proven to be safe and effective in this small case series. No intraocular pressure rise, inflammation or cataracts were registered until last follow-up visit.

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Modified Superior Inverted Internal Limiting Membrane Flap Technique With Vitrectomy Versus Vitrectomy With Internal Limiting Membrane Peeling For Retinal Detachment With Myopic Macular Hole

10.21203/rs.3.rs-913390/v1 ◽

2021 ◽

Author(s):

María José Crespo Carballés ◽

Marina Sastre-Ibáñez ◽

Mar Prieto del Cura ◽

Laura Jimeno Anaya ◽

Natalia Pastora Salvador ◽

...

Keyword(s):

Retinal Detachment ◽

Macular Hole ◽

Case Series ◽

Internal Limiting Membrane ◽

Retinal Layer ◽

Ilm Peeling ◽

Closure Rate ◽

Flap Technique ◽

Before And After ◽

Hole Closure

Abstract Purpose: To evaluate the efficacy of the modified superior inverted internal limiting (ILM) membrane flap technique in retinal reattachment, macular hole closure and external retinal layers restoration in macular hole associated retinal detachment compared to ILM peeling. Methods: Retrospective case series of 10 patients that required pars plana vitrectomy for retinal detachment with macular hole followed for more than 12 months. Data from medical records were retrospectively collected and patients were divided into the superior inverted flap (5 patients) and ILM peeling group (5 patients). We compared best corrected visual acuity (BCVA) before and after surgery, retinal attachment, macular hole closure rate and external retinal layer restoration between groups. Results: There were significant improvements in BCVA in both groups before and after surgery, with no differences between the two groups at 12 months after surgery (p = 0.9). The macular hole closed in 100% of cases in the inverted flap group and 80% of the ILM peeling group with no significant differences between groups. The retina was reattached in 100% of cases in both groups. Only 2 patients in the inverted flap group (40%) had external retinal layer restoration and none in ILM peeling group (p=0,62). Conclusions: ILM peeling and superior inverted flap techniques are useful for treating retinal detachment with macular hole in myopic eyes.

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